2010
DOI: 10.1590/s0103-90162010000300013
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Abstract: The survival of entomopathogenic nematodes under laboratory conditions is low. With the aim of evaluating substrates to extend the survival of entomopathogenic nematodes, suspensions of Heterorhabditis sp. JPM4 and Steinernema carpocapsae All (3,000 IJ mL -1 ) were added to dirt, fine sand, coarse sand, foam, expanded clay, phenolic foam, agar, corn starch, Plantmax®, and water. The substrates were placed on Petri dishes (5 cm) and kept at 16 ± 1°C. Survival evaluations were made after 30, 60, 90, 120, 150, an… Show more

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Cited by 9 publications
(3 citation statements)
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“…When stored in aqueous solution, H. indica has been reported to settle down and form precipitate on the bottom of the container ( Askary et al, 2018 ). Agglomeration leaves the nematodes an environment of low oxygen content and loss of energy reserves which could be a factor for a shorter storage life of native isolate of H. indica ( Andaló et al, 2010 ). A comparison between the different storage temperatures over time reveals that IJ of native isolates H. indica survive better when stored at 15°C, than at any other temperature.…”
Section: Discussionmentioning
confidence: 99%
“…When stored in aqueous solution, H. indica has been reported to settle down and form precipitate on the bottom of the container ( Askary et al, 2018 ). Agglomeration leaves the nematodes an environment of low oxygen content and loss of energy reserves which could be a factor for a shorter storage life of native isolate of H. indica ( Andaló et al, 2010 ). A comparison between the different storage temperatures over time reveals that IJ of native isolates H. indica survive better when stored at 15°C, than at any other temperature.…”
Section: Discussionmentioning
confidence: 99%
“…Infective juvenile survival may be extended by the addition of certain products to IJ suspensions, such as glycerin, which help keep the S. carpocapsae IJs alive and infective for longer periods when compared to water [81] . In addition, some substrates may assist and favor the storage of nematodes, such as the sponge that preserves H. amazonensis JPM4 IJs at 16 °C, with 50% viability after 6 months of storage [82] . An agar-based medium was also tested for the transport and storage of IJs.…”
Section: Formulationmentioning
confidence: 99%
“…(2) Storage temperature: Low-temperature storage is the most common and important factor affecting nematode survival in formulations, but even at low temperatures, nematode species differ in storage long-term of optimum storage temperatures (Strauch et al, 2000;Andalo et al, 2011). Recently, research moved toward improving liquid formulations by using polymer gels (Andalo et al, 2010;Abdel-Aty, 2012 andDe Waal et al, 2013).…”
Section: Introductionmentioning
confidence: 99%