ABSTRACT: Availability of immature embryos as explants to establish wheat (Triticum aestivum L.) by tissue culture can be limited by climatic factors and the lack of high quality embryos frequently hampers experimentation. This study evaluates the effects of rainfall, various temperature-based variables and sunshine duration on tissue culture response (TCR) traits including callus formation (CF), regenerating calli (RC), and number of plants per embryo (PPE) for 96 wheat genotypes of worldwide origin. The obj…
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