Occurence and host specificity of indigenous rhizobia from soils of São Paulo State, Brazil

Lombardi, Maria Luiza Colognesi de Oliveira; Moreira, Milene; Ambrósio, Luís Alberto; Cardoso, Elke Jurandy Bran Nogueira

doi:10.1590/s0103-90162009000400018

Cited by 5 publications

(3 citation statements)

References 19 publications

(10 reference statements)

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“…However, several factors related to the environment and to the symbionts can interfere in successful symbiosis, causing lack of response to inoculation. Among them, native rhizobia populations, physical and chemical properties of the soil, the planting season, and the cultivar may interfere with plant response to BNF (Herridge and Holland, 1993;Sanginga et al, 1996;Mapfumo et al, 2000;Bidlack et al, 2001;Freitas et al, 2003;Lombardi et al, 2009). When evaluating the effectiveness of Bradyrhizobium yuanmingense strains in different sites in the Dominican Republic, Araujo et al (2015) observed significant interaction between the sites where the experiments were carried out and the treatments.…”

Section: Pigeon Pea [Cajanus Cajanmentioning

confidence: 99%

Bradyrhizobium spp. Strains in Symbiosis with Pigeon Pea cv. Fava-Larga under Greenhouse and Field Conditions

Rufini

Oliveira

Trochmann

et al. 2016

Rev. Bras. Ciênc. Solo

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ABSTRACT:Optimization of symbiosis between nitrogen-fixing bacteria and legumes has been extensively studied, seeking agricultural sustainability. To evaluate the symbiotic efficiency of nitrogen-fixing bacterial strains belonging to the Bradyrhizobium genus with pigeon pea (Cajanus cajan (L.) Millsp.) cv. Fava-Larga, experiments were conducted in Leonard jars (axenic conditions), pots with soil, and in the field. Ten strains were tested in Leonard jars, and three strains, in addition to BR 29, were selected according to their ability to promote the growth of pigeon pea, for further tests in pots with different soil types (Inceptsol and Oxisol) and in the field (Oxisol). Treatments were compared with strains BR 2003 and BR 2801 (approved as inoculants for pigeon pea), with a non-inoculated control with mineral N fertilization, and with another non-inoculated control (absolute control) with low mineral N concentration (Leonard jars) or without mineral N fertilization (soil). The efficiency of Bradyrhizobium strains in axenic conditions varies among strains, being higher when pigeon pea cv. Fava-Larga establishes symbiosis with the strains UFLA 03-320, UFLA 03-321, UFLA 04-212, BR 2801, and BR 2003. The soil type influences the symbiotic efficiency of Bradyrhizobium-pigeon pea in soil in the greenhouse, mainly in Inceptsol, in which strains UFLA 04-212, BR 2801, and BR 2003 increased N accumulation in the plant. The strain UFLA 03-320 increased shoot dry matter and N accumulation in the shoot equivalent to the mineral N treatment under field conditions. UFLA 03-320, BR 29, UFLA 03-321, and UFLA 04-212 promoted yields similar to those of the reference strain (BR 2801), and of the mineral N treatment with 70 kg ha -1 urea-N. These results confirm that pigeon pea establishes efficient symbiosis, which provides the N required for its growth. All strains, except for BR 2003, show potential for recommendation as inoculants for grain production. The strain UFLA 03-320 also shows potential for use in green manure crops.

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Section: Pigeon Pea [Cajanus Cajanmentioning

confidence: 99%

Bradyrhizobium spp. Strains in Symbiosis with Pigeon Pea cv. Fava-Larga under Greenhouse and Field Conditions

Rufini

Oliveira

Trochmann

et al. 2016

Rev. Bras. Ciênc. Solo

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“…Some of the main attributes of these species are their rapid and high productivity of biomass (8 Mg ha −1 ) [6][7][8] and their high content of foliar nitrogen, obtained by biological nitrogen fixation (BNF) at an average of 150 kg N ha −1 [9][10][11]. In addition, a characteristic of these species is that they have the ability to establish a promiscuous and functional symbiosis with the native rhizobia of the soil [12]. The biomass production of CCs, including C. juncea and C. spectabilis, is positively correlated with the recycling of nutrients, the entry of carbon (C) into the soil [13][14][15], and a decrease in the rate of erosion [3].…”

Section: Introductionmentioning

confidence: 99%

Cover Crop Selection by Jointly Optimizing Biomass Productivity, Biological Nitrogen Fixation, and Transpiration Efficiency: Application to Two Crotalaria Species

2020

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Crotalaria spectabilis and Crotalaria juncea are cover crops (CC) that are used in many different regions. Among the main attributes of these species are their high potential for biomass production and biological fixation of nitrogen (BNF). Attempting to maximize these attributes, while minimizing water consumption through high transpiration efficiency (TE), is a challenge in the design of sustainable agricultural rotations. In this study, the relationship between biomass productivity, BNF, and TE in C. spectabilis and C. juncea was evaluated. For this purpose, an experiment was carried out under controlled conditions without water limitations and using non-inoculated soil. BNF was determined by the natural abundance of 15N, while TE was estimated by several different methods, such as gravimetric or isotopic method (13C). C. juncea produced 42% less dry matter, fixed 28% less nitrogen from the air, and had 20% less TE than C. spectabilis. TE results in both species were consistent across methodologies. Under simulated environmental conditions of high temperature and non-limiting soil water content, C. spectabilis was a relatively more promising species than C. juncea to be used as CC.

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“…The presence of the compatible rhizobia and their corresponding NF is usually sufficient to trigger nodule formation [ 7 ]. However, successful symbiosis can be affected by several factors, linked to both the environment and the symbiotic partners, which can either promote or prevent nodulation [ 8 ]. Rhizobia enter legume root hairs via the infection thread, a tubular structure that facilitates rhizobial entrance into host root cortical cells [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Classical Soybean (Glycine max (L.) Merr) Symbionts, Sinorhizobium fredii USDA191 and Bradyrhizobium diazoefficiens USDA110, Reveal Contrasting Symbiotic Phenotype on Pigeon Pea (Cajanus cajan (L.) Millsp)

Alaswad

Oehrle

Krishnan

2019

IJMS

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Pigeon pea (Cajanus cajan (L.) Millspaugh) is cultivated widely in semiarid agricultural regions in over 90 countries around the world. This important legume can enter into symbiotic associations with a wide range of rhizobia including Bradyrhizobium and fast-growing rhizobia. In comparison with other major legumes such as soybean and common bean, only limited information is available on the symbiotic interaction of pigeon pea with rhizobia. In this study, we investigated the ability of two classical soybean symbionts—S. fredii USDA191 and B. diazoefficiens USDA110—and their type 3 secretion system (T3SS) mutants, to nodulate pigeon pea. Both S. fredii USDA191 and a T3SS mutant S. fredii RCB26 formed nitrogen-fixing nodules on pigeon pea. Inoculation of pigeon pea roots with B. diazoefficiens USDA110 and B. diazoefficiens Δ136 (a T3SS mutant) resulted in the formation of Fix− and Fix+ nodules, respectively. Light and transmission electron microscopy of Fix- nodules initiated by B. diazoefficiens USDA110 revealed the complete absence of rhizobia within these nodules. In contrast, Fix+ nodules formed by B. diazoefficiens Δ136 revealed a central region that was completely filled with rhizobia. Ultrastructural investigation revealed the presence of numerous bacteroids surrounded by peribacteroid membranes in the infected cells. Analysis of nodule proteins by one- and two-dimensional gel electrophoresis revealed that leghemoglobin was absent in B. diazoefficiens USDA110 nodules, while it was abundantly present in B. diazoefficiens Δ136 nodules. Results of competitive nodulation assays indicated that B. diazoefficiens Δ136 had greater competitiveness for nodulation on pigeon pea than did the wild type strain. Our results suggest that this T3SS mutant of B. diazoefficiens, due to its greater competitiveness and ability to form Fix+ nodules, could be exploited as a potential inoculant to boost pigeon pea productivity.

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Occurence and host specificity of indigenous rhizobia from soils of São Paulo State, Brazil

Cited by 5 publications

References 19 publications

Bradyrhizobium spp. Strains in Symbiosis with Pigeon Pea cv. Fava-Larga under Greenhouse and Field Conditions

Bradyrhizobium spp. Strains in Symbiosis with Pigeon Pea cv. Fava-Larga under Greenhouse and Field Conditions

Cover Crop Selection by Jointly Optimizing Biomass Productivity, Biological Nitrogen Fixation, and Transpiration Efficiency: Application to Two Crotalaria Species

Classical Soybean (Glycine max (L.) Merr) Symbionts, Sinorhizobium fredii USDA191 and Bradyrhizobium diazoefficiens USDA110, Reveal Contrasting Symbiotic Phenotype on Pigeon Pea (Cajanus cajan (L.) Millsp)

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