Screening of Passiflora species for reaction to Cowpea aphid-borne mosaic virus reveals an immune wild species

Abstract

Cowpea aphid-borne mosaic virus (CABMV) is a potyvirus that causes the most serious virus disease of passion fruit crops in Brazil. It is transmitted by several species of aphids in a nonpersistent, non-circulative manner. The reaction of 16 species of Passiflora to infection by mechanical inoculation with four Brazilian isolates of CABMV was evaluated under greenhouse conditions. Only P. suberosa, a wild species, was resistant to infection by all virus isolates, in two independent assays. P. suberosa grafted onto infected P. edulis f. flavicarpa did not develop symptoms; neither was the virus detected by RT-PCR in the upper leaves, suggesting that this species is immune to CABMV.RESUMO: O vírus do mosaico do caupi (Cowpea aphid-borne mosaic virus -CABMV) é um potyvirus que causa uma das mais importantes doenças do maracujazeiro no Brasil. O vírus é transmitido por diversas espécies de afídeos de maneira não persistente, não circulativa. A reação de 16 espécies de Passiflora à infecção com quatro isolados brasileiros do CABMV, por meio de inoculação mecânica foi avaliada em condições de casa-de-vegetação. Somente a espécie selvagem P. suberosa foi resistente à infecção com todos os isolados do CABMV, em dois ensaios independentes. Plantas de P. suberosa enxertadas em plantas de P. edulis f. flavicarpa infectadas com o CABMV também não desenvolveram sintomas da doença. O vírus também não foi detectado por RT-PCR nas folhas superiores das plantas, sugerindo que essa espécie é imune ao CABMV. ULMER, T.; MacDOUGAL, J.M. Passiflora: passionflowers of the world. Cambridge: Timber Press, 2004. 430p.

Genetic Transformation of Passionflower and Evaluation of R1 and R2 Generations for Resistance to Cowpea aphid borne mosaic virus

“…P. suberosa has been reported to be immune to this virus but it presents chrotnosome incompatibility with the cultivated species (20). P. suberosa has been reported to be immune to this virus but it presents chrotnosome incompatibility with the cultivated species (20).…”

Methodological approaches to assess passion fruit resistance (Passiflora spp.) to passionfruit woodiness disease

“…CABMV is transmitted by aphid vectors in a non-circulative, non-persistent manner, (MACIEL et al, 2009;BRAGARD et al 2013). The strategies adopted to date have not been effective to control or eradicate CABMV in commercial passionfruit orchards (FISCHER;CERQUEIRA-SILVA et al 2014), which has limited the expansion of the crop in Brazil (OLIVEIRA et al 2013;CERQUEIRA-SILVA et al 2014;CORREA et al 2015).…”

Efficacy of acibenzolar-S-methyl (Bion®) treatment of Australian commercial passionfruit, Passiflora edulis f. sp. flavicarpa, on resistance to Passionfruit woodiness virus (PWV) and activities of chitinase & β-1,3-glucanase

“…Symptoms of passionfruit woodiness disease include severely distorted, puckered, crinkled leaves, with a mosaic, blisters or yellow lesions on the leaf surface, reduced plant development, and small, deformed, Bwoody^fruit, with abnormally thick, hard rind and reduced pulp (Anderson et al 2009;Cerqueira-Silva et al 2014;Fischer and Rezende 2008;Maciel et al 2009). The potyviral causal agents are transmitted in a non-persistent manner by aphid vectors feeding (Fischer and Rezende 2008;Maciel et al 2009). The viruses are also transmitted by mechanical inoculation, and on grafting and pruning tools used in propagation practices (Fischer and Rezende 2008;Anderson et al 2009).…”

“…Insecticide treatment is not effective as aphids rarely colonize passionfruit in Australia and it also encourages repeated aphid probing, thus increasing virus spread (Hooks et al 2007). Virus control is therefore limited to good sanitation and removal of infected plants (Willingham et al 2002;Anderson et al 2009;Maciel et al 2009) or complete replacement of significantly diseased orchards (Trevisan et al 2006). Cerqueira-Silva et al (2014) reported the current status on classical approaches to improve woodiness disease resistance in Brazil, which incorporate wild Passiflora species as potential sources of resistance.…”