Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost

Chang, Chen-Chin; Ng, Chang-Chai; Wang, Chung-Yi; Shyu, Yuan‐Tay

doi:10.1590/s0103-90162009000300004

Cited by 11 publications

(9 citation statements)

References 26 publications

(26 reference statements)

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“…Enzymatic hydrolysis of cellulose is a promising method for the conversion of waste cellulose to glucose. Many factors, such as the content of lignin, crystallinity of cellulose, degree of polymerization (DP), moisture content and particle size, affect the hydrolytic efficiency of the cellulose present in the lignocellulosic biomass (4–7). Lignin is one of the most abundant polymers in nature.…”

Section: Introductionmentioning

confidence: 99%

“…Several different pretreatment methods have been used to facilitate the subsequent enzymatic hydrolysis of lignocellulosic materials (11, 12). One of the most thoroughly investigated methods is alkali pretreatment in which the alkaline solution is used to remove lignin and various uronic acid substitutions on hemicelluloses, leading to the increased accessibility of enzymes to the hemicellulose and cellulose (4). Generally, alkaline pretreatment is more effective for the subsequent hydrolysis of agricultural residues and herbaceous crops than that for the wood materials (13).…”

Section: Introductionmentioning

confidence: 99%

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Alkali pretreated of wheat straw and its enzymatic hydrolysis

Han

Feng

Zhang

et al. 2012

Braz. J. Microbiol.

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The efficiency of enzymatic hydrolysis of cellulose can be improved by various pretreatments of the substrate. In order to increase the efficiency of enzymatic saccharification of the wheat straw, we determined the effect of different pretreatments on the physical structure, chemical components and enzymatic saccharification of wheat straw. Our results showed that combination of grinding and sodium hydroxide (NaOH) treatment had high effect on the enzymatic hydrolysis of wheat straws. The optimal pretreatment condition was to grind the wheat straws into the sizes of 120 meshes followed by treatment with 1.0% NaOH for 1.5 h (121°C/15psi). Under this condition, the cellulose content of wheat straw was increased by 44.52%, while the content of hemicellulose and lignin was decreased by 44.15% and 42.52%, respectively. Scanning Electronic Microscopy and infrared spectrum analyses showed that significant changes occurred in the structure of wheat straws after pretreatment, which is favorable for the hydrolysis and saccharification. Cellulase produced by Penicillium waksmanii F10–2 was used to hydrolyze the pretreated wheat straw and the optimal condition was determined to be 30 h of enzymatic reaction under the temperature of 55°C, pH 5.5 and substrate concentration of 3%.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Alkali pretreated of wheat straw and its enzymatic hydrolysis

Han

Feng

Zhang

et al. 2012

Braz. J. Microbiol.

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“…Also Patagundi et al [14] isolated a total of 57 isolates from four different agricultural soil samples, three isolates dipicted maximum enzyme activity, they were identified as Bacillus cereus (0.440 U/ml), Bacillus subtilius (0.357 U/ml) and Bacillus thuringiensis (0.334U/ml). While Chang et al [15] isolated five isolates of thermophilic bacteria from cellulosic waste, and two of them (TA-1 and TB-2) were identified as Thermoactinomyces and Bacillus spp. Which showed maximum enzyme activity 2.07 and 2.3 U/ ml respectively.…”

Section: Secondary Screeningmentioning

confidence: 99%

Isolation and Screening of Thermophilic Bacteria for Producing Cellulase Enzyme Using Agricultural Waste

Hussein¹,

Khudhair²,

Mohammed³

2017

JNUS

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Wheat straw was used as a source for isolating thermophilic bacteria and as a substrate for cellulase production. Twenty five of thermophilic bacterial isolates were isolated from agricultural waste samples on Luria-Bertani (L.B) agar plates. These bacterial isolates were qualitatively tested at 50˚C for cellulose production by flooding plates containing mineral salts medium (MSM) with 2% of wheat straw and 0.5% Congo red dye for 15 min followed by repeating wash with 1M NaCl. Results showed that 12 isolates gave a clear zone, after Congo red dye staining of plates; this indicated that the colonies produce cellulase. Bacterial isolates were screened for enzyme activity in submerged liquid MSM with 2% of wheat straw at 50˚C. The produced enzyme is then examined for reducing sugar release in liquid medium. Results revealed that 2T5, 3T13 and 3T18 isolates were the highest in producing cellulase enzyme 5.9, 13.7 and 14.3 Unit/ml, respectively. The most active isolates 2T5, 3T13 and 3T18 were identified on the basis of morphological, biochemical characteristics and API identification kits, they were identified as Bacillus subtilis, Bacillus megaterium and Bacillus licheniformis, respectively.

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“…Chang et al (2009) menemukan bahwa Bacillus sp yang diisolasi dari kompos limbah Brassica memiliki aktivitas selulase berkisar antara 1,9-2,33 U/ml. Demikian pula Pandey et al (2014) dan Ladeira et al (2015) Bacillus clausii adalah bakteri berbentuk batang, merupakan bakteri Gram posit if , m otil dan membentuk endospora (Hema & Shiny, 2012).…”

Section: Figure 4 the Morphology Of Ug3 (Left) Ug7 (Right) And Ug8 unclassified

Seleksi dan Identifikasi Bakteri Selulolitik yang Dapat Mendegradasi Serat Kasar Daun Singkong

Mulyasari

Widanarni

Suprayudi

et al. 2015

JPBKP

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ABSTRAKPemanfaatan daun singkong sebagai bahan baku pakan ikan belum dilaksanakan secara optimal karena mengandung selulosa yang cukup tinggi. Tuj uan penelitian ini adalah mendapatkan bakteri yang dapat mendegradasi serat daun singkong dan melakukan identifikasi jenis bakteri selulolitik tersebut. Seleksi bakteri selulolitik dilakukan pada 4 jenis isolat bakteri asal saluran pencernaan ikan gurame yaitu UG3, UG6, UG7, dan UG8 dengan mengukur aktivitas enzim selulase secara kuantitatif pada substrat Carboxy Methyl Cellulose (CMC) dan daun singkong menggunakan metode 3,5-dinitrosalicylic acid (DNS). Identifikasi bakteri dilakukan pada 3 isolat yang memiliki aktivitas enzim selulase tertinggi pada substrat daun singkong berdasarkan morfologi (perwarnaan Gram, motilitas dan bentuk bakteri), uji biokimia (oksidase dan katalase) dan analisis gen (16S-rRNA). Hasil penelitian menunjukkan bahwa dari 4 isolat yang diuji cobakan, isolat UG7 memiliki aktivitas enzim selulase (CMCase) tertinggi (0,0043 U/ ml) sedangkan yang terendah adalah UG3 (0,0018 U/ml). Pada uji coba di substrat daun singkong, aktivitas enzim tertinggi terlihat pada isolat UG3 (0,107 U/ml) dan terendah adalah UG6 (0,077 U/ ml). Hasil identifikasi dari 3 jenis isolat bakteri uji yang memiliki kemampuan mendegradasi daun singkong tertinggi (UG3, UG7, dan UG8) menunjukkan bahwa ketiga jenis isolat tersebut merupakan bakteri jenis Gram positif berbentuk batang (basil). Berdasarkan data base dari GenBank, isolat UG3 memiliki kemiripan sebesar 93% dengan Bacillus clausii, UG7 memiliki kemiripan sebesar 96% dengan Bacillus amyloliquefaciens dan UG8 memiliki kemiripan sebesar 88% dengan Bacillus subtilis.

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Activity of cellulase from Thermoactinomycetes and Bacillus spp. isolated from Brassica waste compost

Cited by 11 publications

References 26 publications

Alkali pretreated of wheat straw and its enzymatic hydrolysis

Alkali pretreated of wheat straw and its enzymatic hydrolysis

Isolation and Screening of Thermophilic Bacteria for Producing Cellulase Enzyme Using Agricultural Waste

Seleksi dan Identifikasi Bakteri Selulolitik yang Dapat Mendegradasi Serat Kasar Daun Singkong

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