Surface roughness crucially affects the hydrological and erosive behaviours of soils. In agricultural areas surface roughness is directly related to tillage, whose action strongly affects the key physical properties of soils and determines the occurrence and fate of several processes (e.g., surface storage, infiltration, etc.). The characterisation of surface roughness as a result of tillage operations is not straightforward, and numerous parameters and indices have been proposed for quantifying it. In this article, a database of 164 profiles (each 5 m long), measured in 5 different roughness classes, was analysed. Four roughness classes corresponded to typical tillage operations (i.e., mouldboard, harrow, seedbed, etc.), and the fifth represented a seedbed soil that was subject to rainfall. The aim of the research was to evaluate and select the surface roughness parameters that best characterised and quantified the surface roughness caused by typical tillage operations. In total, 21 roughness parameters (divided into 4 categories) were assessed. The parameters that best separated and characterised the different roughness classes were the limiting elevation difference (LD) and the Mean Upslope Depression index (MUD); however, the parameters most sensitive to rainfall action on seedbed soils were limiting slope (LS) and the crossover lengths measured with the semivariogram method (lSMV) and