Genetic linkage maps of chicken chromosomes 6, 7, 8, 11 and 13 from a Brazilian resource population

Abstract

A linkage map is essential not only for quantitative trait loci (QTL) mapping, but also for the organization and location of genes along the chromosomes. The present study is part of a project whose major objective is, besides from construction the linkage maps, the whole genome scan for mapping QTL for performance traits in the Brazilian experimental chicken population. Linkage maps of chicken chromosomes 6 to 8, 11 and 13 were constructed based on this population. The population was developed from two generations of crossbreeding between a broiler and a layer line. Fifty-one microsatellite markers were tested, from which 28 were informative: 4, 8, 7, 4 and 5 for chromosomes 6, 7, 8, 11 and 13, respectively. A SNP located in the leptin receptor gene was included for chromosome 8. Ten parental, 8 F 1 and 459 F 2 chickens from five full-sib families were genotyped with these markers. The number of total informative meioses per locus varied from 232 to 862, and the number of phaseknown informative meioses from 0 to 764. Marker orders in the chromosomes coincided with those of the chicken consensus map, except for markers ADL0147 and MCW0213, on chromosome 13, which were inverted. The reduced number of phase-known informative meioses for ADL0147 (150) may be pointed out as a possible cause for this inversion, apart from the relative short distance between the two markers involved in the inversion (10.5 cM).

Genotypic characterization of microsatellite markers in broiler and layer selected chicken lines and their reciprocal F1s

“…These populations have been used for QTL mapping for performance and carcass traits Ruy et al, 2005;Ambo et al, 2008).…”

Identification of polymorphisms associated with production traits on chicken (Gallus gallus) chromosome 4

“…With the advantage of DNA investigation technologies, the ability to identify molecular markers that are used to construct linkage maps has improved (Mackay, 2001), allowing the detection of hundreds of quantitative trait loci (QTLs; dbQTL, http://www.animalgenome.org/cgibin/QTLdb/index). Several studies using microsatellite markers have identified QTLs associated with production traits across the chicken genome (Zhou et al, 2006;Ambo et al, 2008Ambo et al, , 2009Campos et al, 2009;Ankra-Badu et al, 2010;Baron et al, 2010;Nones et al, 2012;Nassar et al, 2013). Other studies that have used single nucleotide polymorphism (SNP) markers have identified genetic associations and linkage with production, health, and behavioral traits in farm animals (Zanella et al, 2011;McSpadden et al, 2013).…”

Genome-wide association study for performance traits in chickens using genotype by sequencing approach

“…Several studies have been conducted in chickens using markers randomly distributed in the genome (microsatellites), which have allowed the identification of several QTLs for production traits 3 4 5 6 7 8 9 10 11 12 13 . Following these, some of the studies have focused their attention on the identification of SNPs in functional and positional candidate genes and to test their association on target QTL regions 14 15 16 17 18 19 .…”