Association of PIT1 genotypes with growth traits in Canchim cattle

Abstract

Use of genetic markers can aid on the identification of animals with highest breeding values in beef cattle. The PIT1 gene codes for the Pit-1 transcription factor is essential for the activation of prolactin, growth hormone and PIT1 genes. This research is an investigation of the effect of PIT1-HinfI polymorphism on growth traits of 509 Canchim animals, from two lineages, GG1 and GG2. PIT1 genotypes were identified through PCR-RFLP. Genotype effect on phenotypic values for birth weight (BW), standardized weaning weight (W240), weight at 12 months of age (W365), and the average daily weight gain from birth to weaning (AGBW), and from weaning to 12 months of age (AGW12) were analyzed by the least squares method. Effects of the interaction between the animal's genetic group and PIT1 genotype for W240, AGBW and AGW12 were observed (P < 0.05). Differences between means of HinfI (-/-), HinfI (+/+) and HinfI (+/-) genotypes for W240 and AGBW were observed in GG2 (P < 0.05), revealing superiority of (-/-) genotype for those traits. Means for genotypes (+/+) and (+/-) for W240 and AGBW, did not differ from one another, suggesting a dominance effect of the HinfI (+) allele. The HinfI (-) allele had a favorable effect on W240 and AGBW in GG2, when present in homozygosis. The difference between PIT1 behavior observed in the two genetic groups may suggest the action of a quantitative trait locus linked to PIT1, segregating only in GG2 population. Key words: RFLP, bovine, pituitary factor 1, candidate gene ASSOCIAÇÃO DE GENÓTIPOS PIT1 COM CARACTERÍSTICAS DE CRESCIMENTO EM GADO CANCHIMRESUMO: O uso de marcadores genéticos pode favorecer a identificação de animais geneticamente superiores em bovino de corte. O gene PIT1 codifica para o fator de transcrição Pit-1, crítico para ativar a expressão dos genes da prolactina, hormônio de crescimento e PIT1. Esta pesquisa investiga o efeito do polimorfismo HinfI-PIT1 sobre características de crescimento em 509 animais da raça Canchim, pertencentes à duas linhagens, GG1 e GG2. Os genótipos do gene PIT1 foram identificados pela técnica PCR-RFLP. Os efeitos dos genótipos sobre valores fenotípicos para pesos ao nascimento (PN), pesos padronizados à desmama (P240) e ao ano (P365), ganhos médios diários de peso do nascimento à desmama (GMND), e da desmama aos 12 meses de idade (GMD12) foram analisados pelo método dos quadrados mínimos. Foram observados efeitos de interação entre grupo genético do animal e genótipo de PIT1 sobre P240, GMND e GMD12 (P < 0.05). Diferenças entre médias dos genótipos HinfI (-/-), HinfI (+/+) e HinfI (+/-) para P240 e GMND foram observadas em GG2 (P < 0,05), revelando superioridade do genótipo HinfI (-/-). As médias dos genótipos (+/+) e (+/-) para P240 e GMND, não diferiram, sugerindo efeito de dominância do alelo HinfI (+). O alelo HinfI (-) mostrou-se favorável sobre P240 e GMND em GG2, quando em homozigose. A diferença de comportamento de PIT1 observada nos dois grupos genéticos pode sugerir a ação de loco de característica quantitativa ligado ao ge...

Effect of IGF1, GH, and PIT1 markers on the genetic parameters of growth and reproduction traits in Canchim cattle

“…Our findings did not confirm the existence of associations of production and reproduction traits with molecular markers for IGF1, GH, and PIT1 and disagreed with what has been reported about the effect of these genes, especially on growth traits in Canchim cattle [14,21,37]. The difference between our results and the ones previously Table 2 Number of animals with phenotypic information (N), heritabilities estimates and proportion of the phenotypic variation due to the IGF1, GH or PIT1 markers estimated in the Canchim found for the Canchim breed can be due to the methodology applied.…”

“…and Hinf-) were defined by a silent mutation [19]. The DNA amplification techniques for the markers on IGF1 and GH [20], the method for PIT1 amplification [21], and the primers for IGF1 [22], GH [23], and PIT1 [24] markers have been previously described.…”

“…Zhang et al [46] found no significant association (p [ 0.05) of the POU1F1 gene (formerly named PIT1) with body weight traits measured at four months of age in Qinchuan breed and its crossings with Angus, Limousin, and Germany Yellow breeds. Other studies showed association of PIT1 with body weight in one line of Canchim cattle [21], in double-muscled Belgian Blue cattle [47], and in an F2 Holstein x Gyr population [38].…”

Analysis of selected milk traits in Palestinian Holstein-Friesian cattle in relation to genetic polymorphism

“…In cattle, PIT-1 was found in several studies to be associated with body weight and average daily gains (Renaville et al, 1997;Carrijo et al, 2008) and milk production traits (Renaville et al, 1997;De Mattos et al, 2004;Xue et al, 2006). Other studies, however, failed to verify the association between PIT-1 and production traits (Di Stasio et al, 2002;Dybus et al, 2004;Zhao et al, 2004;Selvaggi and Dario, 2011).…”

Association between IGF2 and CYP21 gene polymorphisms and characteristics of economic interest in Nellore cattle

“…The direct application of molecular techniques has allowed the detection of SNPs (single nucleotide polymorphisms), a type of polymorphism that is characteristically abundant in the bovine genome, as evidenced in researches of genotypephenotype association to observe genotypic variations related to phenotypic characteristics (Andréa et al, 2007;de Souza et al, 2007;Carrijo et al, 2008;Caetano, 2009;Curi et al, 2009).…”