Molecular confirmation of Maize rayado fino virus as the Brazilian corn streak virus

Hammond, Rosemarie W.; Bedendo, Ivan Paulo

doi:10.1590/s0103-90162005000600015

Cited by 9 publications

(2 citation statements)

References 15 publications

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“…Dalbulus maidis can cause up to 98% yield loss in maize by direct feeding on phloem sap and pathogen transmission (Nault & Bradfute, 1979;Pérez-López et al, 2018;Summers et al, 2004;Tsai, 2008). At least seven vector-borne diseases are known in maize, five of which are viral and two bacterial (Carloni et al, 2013;Hammond & Bedendo, 2005;Liu & Wang, 2018;Meyer & Pataky, 2010;Orlovskis et al, 2017;Zhang et al, 2011). Dalbulus maidis is the insect vector responsible for transmitting three of the most important maize disease agents, namely Maize rayado fino virus (MRFV), Maize bushy stunt phytoplasma, and Spiroplasma kunkelii (CSS), which it can acquire and transmit together or individually (Galvão et al, 2020;Mlotshwa et al, 2020;Sabato et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Assessing the Functionality of RNA Interference (RNAi) in the Phloem-feeding Maize pestDalbulus maidis

Medina

2021

Preprint

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Dalbulus maidis [(DeLong & Wolcott), corn leafhopper], a phloem-feeding insect, is the most efficient vector of maize stunting pathogens (Spiroplasma kunkelii, Maize bushy stunt phytoplasma, and Maize rayado fino virus) in the Americas. Studies involving gene editing in insects are rapidly providing information that can potentially be used for insect vector and plant disease control. RNA interference (RNAi), a sequence-specific gene silencing method, is one of the most widely used molecular tools in functional genomics studies. RNAi uses exogenous double-stranded RNA (dsRNA) or small interfering RNA (siRNA) to prevent the production of proteins by inhibiting the expression of their corresponding messenger RNA (mRNA). In this study, we measured the efficacy of gene silencing, and its effects on D. maidis mortality as proof of concept that RNAi is a viable tool for use in genetic pest control of phloem-feeding insects. Oral delivery of dsRNA using an artificial diet was used to silence two key insect genes, vacuolar ATP synthase subunit B, and subunit D (V-ATPase B and V-ATPase D). Our results showed reduced gene expression of V-ATPase B and V-ATPase D after ingestion of dsRNA, and significantly higher mortality, and wing deformation, associated with reduced gene expression, compared to control insects that were not orally fed dsRNA. These results reveal RNAi as a viable tool for use in genetic pest control of phloem-feeding insects, and a way for further functional genomic studies, such as identification of potential target genes for either population suppression or population replacement of this vector of maize diseases.

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Section: Introductionmentioning

confidence: 99%

Assessing the Functionality of RNA Interference (RNAi) in the Phloem-feeding Maize pestDalbulus maidis

Medina

2021

Preprint

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“…More than 700 distinct plant diseases identified as vector-borne pose a significant threat to plant health and food security within agricultural systems all over the world [ 1 , 2 , 3 , 4 ]. The study of interactions between insect vectors, phytopathogens, and their shared plant hosts is fundamental for the elucidation of clues necessary for developing novel and sustainable control strategies to mitigate losses caused by vector-borne plant diseases.…”

Section: Introductionmentioning

confidence: 99%

Corn Stunt Disease: An Ideal Insect–Microbial–Plant Pathosystem for Comprehensive Studies of Vector-Borne Plant Diseases of Corn

Jones

Medina

2020

Plants

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Over 700 plant diseases identified as vector-borne negatively impact plant health and food security globally. The pest control of vector-borne diseases in agricultural settings is in urgent need of more effective tools. Ongoing research in genetics, molecular biology, physiology, and vector behavior has begun to unravel new insights into the transmission of phytopathogens by their insect vectors. However, the intricate mechanisms involved in phytopathogen transmission for certain pathosystems warrant further investigation. In this review, we propose the corn stunt pathosystem (Zea mays–Spiroplasma kunkelii–Dalbulus maidis) as an ideal model for dissecting the molecular determinants and mechanisms underpinning the persistent transmission of a mollicute by its specialist insect vector to an economically important monocotyledonous crop. Corn stunt is the most important disease of corn in the Americas and the Caribbean, where it causes the severe stunting of corn plants and can result in up to 100% yield loss. A comprehensive study of the corn stunt disease system will pave the way for the discovery of novel molecular targets for genetic pest control targeting either the insect vector or the phytopathogen.

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Discovery of the first maize-infecting mastrevirus in the Americas using a vector-enabled metagenomics approach

et al. 2017

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The genus Mastrevirus (family Geminiviridae) is composed of single-stranded DNA viruses that infect mono- and dicotyledonous plants and are transmitted by leafhoppers. In South America, there have been only two previous reports of mastreviruses, both identified in sweet potatoes (from Peru and Uruguay). As part of a general viral surveillance program, we used a vector-enabled metagenomics (VEM) approach and sampled leafhoppers (Dalbulus maidis) in Itumbiara (State of Goiás), Brazil. High-throughput sequencing of viral DNA purified from the leafhopper sample revealed mastrevirus-like contigs. Using a set of abutting primers, a 2746-nt circular genome was recovered. The circular genome has a typical mastrevirus genome organization and shares <63% pairwise identity with other mastrevirus isolates from around the world. Therefore, the new mastrevirus was tentatively named "maize striate mosaic virus". Seventeen maize leaf samples were collected in the same field as the leafhoppers, and ten samples were found to be positive for this mastrevirus. Furthermore, the ten genomes recovered from the maize samples share >99% pairwise identity with the one from the leafhopper. This is the first report of a maize-infecting mastrevirus in the Americas, the first identified in a non-vegetatively propagated mastrevirus host in South America, and the first mastrevirus to be identified in Brazil.

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Molecular confirmation of Maize rayado fino virus as the Brazilian corn streak virus

Cited by 9 publications

References 15 publications

Assessing the Functionality of RNA Interference (RNAi) in the Phloem-feeding Maize pestDalbulus maidis

Assessing the Functionality of RNA Interference (RNAi) in the Phloem-feeding Maize pestDalbulus maidis

Corn Stunt Disease: An Ideal Insect–Microbial–Plant Pathosystem for Comprehensive Studies of Vector-Borne Plant Diseases of Corn

Discovery of the first maize-infecting mastrevirus in the Americas using a vector-enabled metagenomics approach

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