2005
DOI: 10.1590/s0103-90162005000100009
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Abstract: Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim of the present study was to identify the species of Aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the State of Paraná, in the South of Brazil. A total of 108 isolates of Aspergillus spp. was identified at the … Show more

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Cited by 36 publications
(22 citation statements)
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“…The amplifi cation was based on the methodology described by Magnani et al (2005). The amplifi ed products at the rDNA regions ITS1-5,8S-ITS2 of the isolated endophytic fungi were purifi ed with GFX PCR DNA and Gel Band Purification (Amersham Biosciences), according to the manufacture's instructions.…”
Section: Dna Extraction Rdna Amplifi Cation and Sequencingmentioning
confidence: 99%
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“…The amplifi cation was based on the methodology described by Magnani et al (2005). The amplifi ed products at the rDNA regions ITS1-5,8S-ITS2 of the isolated endophytic fungi were purifi ed with GFX PCR DNA and Gel Band Purification (Amersham Biosciences), according to the manufacture's instructions.…”
Section: Dna Extraction Rdna Amplifi Cation and Sequencingmentioning
confidence: 99%
“…The sequencing reaction of the ITS rDNA regions was under the same conditions as described by Magnani et al (2005). The sequencing was carried out with a MegaBace TM 1000 sequencer (Amersham Biosciences).…”
Section: Dna Extraction Rdna Amplifi Cation and Sequencingmentioning
confidence: 99%
See 1 more Smart Citation
“…The amplification of ITS regions of rDNA followed the methodology by Magnani et al (2005). The amplified fragments were analyzed by 1% agarose gel electrophoresis.…”
Section: Amplification and Purification Of Rdna's Itsmentioning
confidence: 99%
“…Sequencing of the rDNA ITS1 -5.8S -ITS2 region was undertaken following Magnani et al (2005). The amplification was performed in a MJ thermocycler (MJ Research, Inc.PTC-100) and the sequencing was achieved in MegaBace TM 1000 sequencer (Amersham Biosciences).…”
Section: Sequencing Of the Rdna Its Regions And Phylogenetic Analysismentioning
confidence: 99%