2004
DOI: 10.1590/s0103-90162004000600012
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Nitrate reductase activity in leaves and stems of tanner grass (Brachiaria radicans Napper)

Abstract: Tanner grass (Brachiaria radicans Napper) is a forage plant that is adapted to well-drained soils or wetlands, and responds well to nitrogen (N) fertilization. The assimilation of N involves the nitrate reductase (NR) enzyme, and its activity seems to be dependent on N supply. Molybdenum (Mo) is also important because it is a cofactor of NR. In this study, the variables of an in vivo assay were optimized for measuring nitrate reductase activity (NRA) in the leaves and stem tissues. This method was used to eval… Show more

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Cited by 29 publications
(7 citation statements)
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“…This result is in line with several reports, namely leaf NRA correlates with NO 3 content in soil and stem tissue [20]. Nitrogen sources such as NaNo 3 produced the highest of NRA compared to nitrogen sources of NH 4 Cl and Urea [21]. The increase in N accumulation in the root or leaf tissue by fertilization stimulated NRA in both leaves and roots 13 and 42%, respectively [22].…”
Section: Effect Of Npk Fertilizer Treatment Onsupporting
confidence: 92%
“…This result is in line with several reports, namely leaf NRA correlates with NO 3 content in soil and stem tissue [20]. Nitrogen sources such as NaNo 3 produced the highest of NRA compared to nitrogen sources of NH 4 Cl and Urea [21]. The increase in N accumulation in the root or leaf tissue by fertilization stimulated NRA in both leaves and roots 13 and 42%, respectively [22].…”
Section: Effect Of Npk Fertilizer Treatment Onsupporting
confidence: 92%
“…Activity of the enzyme nitrate reductase was measured in the fourth developed leaf from the apex of the plants with the nitrate N source. For this the leaves were collected between 10:00 and 11:00 h in the morning and soon after the analysis was performed in triplicate with the leaves in vivo according to the methodology proposed by (Cazetta and Villela, 2004).…”
Section: Methodsmentioning
confidence: 99%
“…The application of ammonium sulfate significantly increased the NRA in soybean. The assimilation of N involves the NRA, and its activity seems to be dependent on the N availability in the soil (Cazzeta and Villela, 2004). Application of ammonium sulfate as much as 100 kg ha -1 to corn significantly increases NRA by 79.430 mmol kg -1 h -1 FW in comparison to the without ammonium sulfate, with NRA value of 35.96 mmol kg -1 h -1 FW (Purbajanti et al, 2016).…”
Section: Discussionmentioning
confidence: 99%