Micropropagation and callogenesis of Curcuma zedoaria Roscoe

Miachir, Jeanette Inamine; Romani, V L M; Amaral, Antônio Francisco de Campos; Mello, Marcia O.; Crocomo, Otto J.; Melo, Murilo

doi:10.1590/s0103-90162004000400012

Cited by 16 publications

(7 citation statements)

References 16 publications

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“…This shows an inhibitory effect of NAA auxin (in the concentrations used in this work) on the organogenesis of the species under analysis. Similar results were found for Curcuma zedoaria Roscoe (Miachir et al, 2004) and four species of the genus Watsonia (Ascough et al, 2007).…”

Section: Resultssupporting

confidence: 78%

In vitro morphogenesis of Syngonanthus mucugensis Giul: subsp. mucugensis

et al. 2011

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Syngonanthus mucugensis Giul. subsp. mucugensis is an herbaceous plant with significant economic value in the ornamental dry flower business. The restricted occurrence of the municipality Mucugê-BA, Brazil, exclusively associated with extractive exploitation, has considered this species as endangered. The objective of this work was to evaluate the organogenic potential of three different types of S. mucugensis subsp. mucugensis explants to promote the development of an alternative method to the propagation of the genetic resources of this important plant. The morphogenetic capacities of the leaf, stem and root this species was tested using Murashige and Skoog culture medium at half salt concentration and different concentrations of growth of regulators benzylaminopurine - BAP (0.00; 2.22 and 4.44 µM), and naphthalene acetic acid - NAA (0.00; 1.34 and 2.68 µM). The morphoanatomic events that lead to formation of shoots were described. Stems proved to be the best source of explants, showing 58.75% regeneration of shoot by direct organogenesis in the absence of growth regulators, and 32.18 and 47.55% of shoot regeneration by indirect organogenesis in the presence of 2.22 and 4.44 µM BAP, respectively. As for leaves, there was callus formation, but without regenerating shoots. Morphogenesis was not observed when roots were used as explants. The histological analyses showed that shoot regeneration in S. mucugensis subsp. mucugensis occurred both indirectly, by unorganized tissue differentiation, and directly through returning to merismatic activity in differentiated mature cells and preexisting bud proliferation.
Syngonanthus mucugensis Giul. subsp. mucugensis é uma herbácea com grande potencial de utilização no comércio de flores secas ornamentais. A ocorrência restrita ao município de Mucugê-BA, Brasil, associado à exploração extrativista tem levado essa espécie ao risco de extinção. Neste estudo, objetivou-se avaliar o potencial organogênico de três diferentes tipos de explantes de S. mucugensis subsp. mucugensis visando ao desenvolvimento de um método alternativo para a sua propagação. A capacidade morfogênica de caule, folha e raiz foi testada utilizando o meio de cultura Murashige e Skoog com metade da concentração salina e diferentes concentrações dos reguladores de crescimento benzilaminopurina - BAP (0,00; 2,22 e 4,44 µM) e ácido naftaleno acético - ANA (0,00; 1,34 e 2,68 µM). Os eventos morfoanatômicos que levaram a formação dos brotos foram descritos. O caule demonstrou ser a melhor fonte de explante, apresentando 58,75% de regeneração de brotos via organogênese direta, em meio livre de regulador de crescimento; e 32,18 e 47,55% de regeneração de brotos por organogênese indireta na presença de 2,22 e 4,44 µM de BAP, respectivamente. Para folha, foi obtida a formação de calos sem regeneração de brotos, não sendo observada morfogênese, quando se utilizou raiz como explante. As análises histológicas mostraram que a regeneração de brotos em S. mucugensis subsp. mucugensis ocorreu tanto por via indireta, pela...

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Section: Resultssupporting

confidence: 78%

In vitro morphogenesis of Syngonanthus mucugensis Giul: subsp. mucugensis

et al. 2011

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“…All the successful induction of callus in this study were obtained in 2, 4-D + BAP supplemented MS media. 2, 4-D alone failed to induce calluses at all the concentration tested (0.5, 1….3 mg/L) which was in line with the report of Zuraida et al, (2014) in C. caesia and also with the finding of Miachir et al, (2004) and Tuan et al, (2011) in C. zedoaria, however, in contradiction to the finding of Saensouk, (2011) were similar and differed from all others (p <0.05). Equal and higher concentration 2,4-D + BAP of 5 mg/L each, responded well (40.00%) to callus induction in contrast to the finding of Taha et al, (2013) who reported higher concentration of 2,4-D up to 4 mg/L in combination with 0.2 mg/L BAP to have performed less in the in vitro culture of Zingiberofficinale using leaves explants.…”

Section: Resultssupporting

confidence: 85%

Sterilization procedure and callus regeneration in black turmeric (Curcuma caesia)

Abubakar

Pudake

2019

ASD

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Sterilization procedure, media composition, explants selection and control of physical environment are critical for successful cultures and callus induction with surface sterilization being very challenging in most plants. Five different sterilization methods were evaluated to come up with the best for subsequent use to establish an in vitro regeneration method for the induction of callus in Curcuma caesia using excised leaf and rhizome explants. Murashige and Skoog (MS) media supplemented with various concentration of 2,4-Dichlorophenoxy acetic acid (2,4-D)/Indole-3-acetic acid (IAA) (0.5- 5.0mg/L), singly or in combination with Benzyl aminopurine (BAP)/Kinetin (KIN) (0.1-5.0mg/L), 0.3% sucrose and 0.08% agar were used. The result of the sterilization procedures showed 15% NaHClO3 (5min) + 70% Ethanol (30s) + 0.1% HgCl2 (5min) to be the most effective in controlling contamination in C. caesia among all the treatments tested. The response to callus induction was found to depend on the type of explants used and growth regulators combination. Leaf explants gave the highest percentage of callus induction. Highest percentage of callus induction (66.70%) was obtained in the growth regulator combination of 2, 4-D (0.5mg/L) + BAP (0.1mg/L) and least (14.29%) in IAA (2.0mg/L) + BAP (0.5mg/L). Equal and higher concentration of 2, 4-D + BAP of 5.0mg/L each also provided better result (40.00%). No callus was obtained in all the single concentration of 2, 4-D used.

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“…1955). This shows that the conventional multiplication capacity of C. longa is very limited compared to other propagation methods (Miachir et al, 2004).…”

Section: Introductionmentioning

confidence: 94%

Growth Regulators Affect the Growth and Biochemical Activity of Curcuma longa Plants Grown in vitro

Ferrari¹,

Queiroz²,

Andrade³

et al. 2019

JAS

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This study aimed to evaluate the in vitro growth and biochemical activity of Curcuma longa explants using different MS medium formulations and growth regulators. In all the experiments, plants were grown in MS medium supplemented with agar (6.5 g L-1) and pH adjusted to 5.8. In the first assay, the MS culture medium at 70% strength, supplemented with 30 g L-1 of sucrose, and without the addition of activated carbon, resulted in the highest number of shoots. The sucrose concentration of 60 g L-1, combined with the addition of actived charcoalin half-strength MS medium, resulted in the increased root dry mass, root collar diameter, and relative chlorophyll index. In the second assay, the highest root collar diameter and dry matter of shoots and roots were found in the MS medium supplemented with 4.44 BAP, 0.46 KIN, and 1.08 NAA. The MS medium with 8.88 BAP, 0.92 KIN, and 2.16 μM NAA resulted in the highest number of shoots (7.75), number of leaves (35), and shoot length (88.57 mm). The antioxidant activity was significantly higher in the treatments that resulted in better plantlets growth performance, demonstrating that the antioxidant activity is related to other factors such as a possible role of growth regulators on the elicitation of compounds in plants. Superoxide dismutase had a high enzymatic activity in both assays, whereas the enzymatic activity of catalase and ascorbate peroxidase was dependent on the culture media used.

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Micropropagation and callogenesis of Curcuma zedoaria Roscoe

Cited by 16 publications

References 16 publications

In vitro morphogenesis of Syngonanthus mucugensis Giul: subsp. mucugensis

In vitro morphogenesis of Syngonanthus mucugensis Giul: subsp. mucugensis

Sterilization procedure and callus regeneration in black turmeric (Curcuma caesia)

Growth Regulators Affect the Growth and Biochemical Activity of Curcuma longa Plants Grown in vitro

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