Microsatellite markers for identification of Prunus spp. rootstocks

Bianchi, Valmor João; Sansavini, S.; Fachinello, José Carlos

doi:10.1590/s0103-90162004000300011

Cited by 12 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, additional markers for identification may be useful and have been developed in several organisms. Most of them are specific DNA or RNA sequences obtained by different techniques (Li et al 2002;Randig et al 2004;Barik et al 2006;Vargas et al 2005;Bianchi et al 2004). Speciesmarker bands in native and SDS-PAGE gel electrophoresis of soluble proteins were described in fish by Hasnain et al (2005).…”

Section: Discussionmentioning

confidence: 99%

Variability of esterase patterns in adult flies of the saltans species group of Drosophila (subgenus Sophophora)

Bernardo

Bicudo

2009

Genetica

View full text Add to dashboard Cite

Esterases are known for their involvement in several physiological processes and high degree of polymorphism, in many organisms. Such polymorphism has been used to characterize species and species groups and to study genetic changes occurred in their evolutionary history. In the present study, the esterase patterns of 19 strains from 10 species representative of the five subgroups of the saltans species group were analyzed using polyacrylamide gel electrophoresis and alpha- and beta- naphthyl acetates as substrates. Fifty-one esterase bands were detected and classified as 31 alpha-esterases, 18 beta-esterases and two alpha/beta-esterases. On the basis of the inhibition patterns using Malathion and eserine sulfate, 34 bands were classified as carboxylesterases, 14 as acethylesterases and three as cholinesterases. Ten gene loci were tentatively established on the basis of data on band position in the gel, substrate preference and inhibition pattern. Twenty bands were species-specific, the remaining being shared by species from the same or different subgroups. Bands detected exclusively in males and bands with a different frequency or degree of expression between sexes were also detected. In the gels prepared for analysis of gene expression in the body parts (head, thorax and abdomen), the degree of expression of the beta-esterases was higher in the thorax, while the alpha-esterases were expressed predominantly in the abdomen and thorax. A global view of the data available at present on the esterases of the species from the saltans group and their degree of polymorphism are presented, as well as the possibility of using some beta-esterases, because of their characteristics in the gels, as markers for species identification.

show abstract

Section: Discussionmentioning

confidence: 99%

Variability of esterase patterns in adult flies of the saltans species group of Drosophila (subgenus Sophophora)

Bernardo

Bicudo

2009

Genetica

View full text Add to dashboard Cite

show abstract

“…(Fang & Roose, 1997), grape (Lopez et al, 1999), cherry (Cantini et al, 2001;Struss et al, 2002), and pear (Monte-Corvo et al, 2001). Moreover, they have been used to study genetic diversity and relationships in apple (Hokanson et al, 1998), sweet cherry (Dirlewanger et al, 2002), Japanese apricot (Gao et al, 2004), plum (Ahmad et al, 2004b;Rohrer et al, 2004), almond (Xu et al, 2004), peach cultivars and rootstocks (Ahmad et al, 2004a;Bianchi et al, 2004;Marchese et al, 2005). They also have been efficient in testing the genetic origin of peach cultivars (Testolin et al, 2000) and the reconstruction of a grape pedigree (Sefc et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Simple Sequence Repeat Markers for Detecting Sources of Tolerance to PTSL Syndrome in Prunus Persica Rootstocks

et al. 2006

View full text Add to dashboard Cite

Peach tree short life (PTSL) is a severe and complex disease syndrome in the Southeast of the United States that causes significant damage to peach orchards and affects more than 70% of the peach acreage in the region. The ring nematode is known to be one of the major factors strongly associated with the PTSL syndrome. A peach rootstock (Guardian R Brand BY520-9, also referred to as Guardian R or BY520-9), which is tolerant to ring nematode and PTSL, was released in 1994. To investigate the genetic relatedness of PTSL tolerant genotypes and identify possible parents of Guardian R selections as sources of the PTSL tolerance trait, 98 genotypes derived from the BY520-9 pedigree, and others showing different degrees of field resistance or susceptibility to PTSL, were selected for simple sequence repeat analysis. Nineteen of 32 microsatellite loci were polymorphic. Allele number averaged 2.7 per locus with heterozygosity levels ranging from 0.07 to 0.60. Cluster analysis of these genotypes produced a dendrogram in agreement with their putative pedigrees. Thirty-eight F 4 genotypes from Guardian pedigree were used for the parental identification analyses. Of those, nine most likely parental genotypes were identified for 10 field tested F 5 genotypes produced from open-pollinated selections of BY520-9.

show abstract

“…Bianchi et al (2004b) verificaram problemas de identificação inter e intravarietal de porta-enxertos de pessegueiro [Prunus persica (L.) Batsch], no Banco de Germoplasma da Universidade Federal de Pelotas (UFPel), o que reforça a necessidade de comprovação da origem genética do material presente nesses bancos. Além disso, a identificação da variabilidade entre genótipos em bancos de germoplasma também tem se mostrado importante, como verificado nos trabalhos de Sosinski et al (2000), Testolin et al (2000), Aranzana et al (2002) e Bouhadida et al (2009.…”

Section: Introductionunclassified

Caracterização molecular e variabilidade genética entre porta-enxertos de pessegueiro com base em marcadores codominantes

Paula

Bianchi²,

Fachinello

2012

Pesq. agropec. bras.

Self Cite

View full text Add to dashboard Cite

Resumo -O objetivo deste trabalho foi estabelecer um padrão para a caracterização molecular e a diferenciação de porta-enxertos de pessegueiro, bem como estimar parâmetros de variabilidade genética com base em marcadores codominantes. Catorze genótipos foram avaliados com uso de iniciadores para locos microssatélites das séries BPPCT e UDP, e para locos STS e SCAR. O perfil eletroforético foi registrado quanto à presença ou à ausência de bandas, as quais foram usadas para calcular a similaridade genética entre cultivares, por meio do coeficiente "simple matching", e para a análise de agrupamento de cultivares, pelo método das médias aritméticas não ponderadas (UPGMA). Foram calculados: número de alelos por loco, frequências alélicas, heterozigosidade esperada e observada, endogamia e conteúdo de informação polimórfica (PIC). Os 18 locos avaliados produziram 82 polimorfismos e permitiram elaborar um dendrograma que discriminou os genótipos em três grupos principais. A heterozigosidade esperada e observada nos 18 locos SSR foi de 0,66 e 0,22, respectivamente. Valores máximos para endogamia (1,0) foram identificados nos locos UDP 98407, UDP 98412, BPPCT 034, BPPCT 016, SCAR-SCAL 19 e STS OPAP4. O PIC variou de 0,81, para SCAR-SCAL 19, a 0,46, para UDP 98407. O polimorfismo dos 18 marcadores possibilita obter acurada relação genética entre os genótipos de pessegueiro avaliados e identificar os mais contrastantes para uso em programas de melhoramento. Termos de indexação: Prunus persica, distância genética, frequência alélica, genética molecular, seleção de parentais. Molecular characterization and genetic variability among peach rootstocks based on codominant markersAbstract -The objective of this work was to establish a standard for molecular characterization and differentiation of peach rootstocks, as well to estimate genetic variability parameters based on codominant markers. Fourteen genotypes were evaluated using primers for microsatellite loci from the BPPCT and UDP series and for the STS and SCAR loci. The electrophoretic profile was recorded for the presence or absence of bands, which were used to calculate the genetic similarity among cultivars by the simple matching coefficient, and to perform cluster analysis by the unweighted arithmetic average (UPGMA) method. The number of alleles per locus, allele frequencies, observed and expected heterozygosity, inbreeding, and polymorphic information content (PIC) were calculated. The 18 loci produced 82 polymorphisms, allowing for the elaboration of a dendrogram, which discriminated the genotypes into three main groups. Observed and expected heterozygosity in the 18 SSR loci were 0.66 and 0.22, respectively. Maximum values for inbreeding (1.0) were identified in loci UDP 98407, UDP 98412, BPPCT 034, BPPCT 016, SCAR-SCAL 19, and STS OPAP4. PIC ranged from 0.81 for SCAR-SCAL 19, to 0.46 for UDP 98407. The polymorphism of the 18 markers allows for an accurate genetic relationship among the evaluated peach genotypes and the identification of the more contrasting ones f...

show abstract

Microsatellite markers for identification of Prunus spp. rootstocks

Cited by 12 publications

References 4 publications

Variability of esterase patterns in adult flies of the saltans species group of Drosophila (subgenus Sophophora)

Variability of esterase patterns in adult flies of the saltans species group of Drosophila (subgenus Sophophora)

Simple Sequence Repeat Markers for Detecting Sources of Tolerance to PTSL Syndrome in Prunus Persica Rootstocks

Caracterização molecular e variabilidade genética entre porta-enxertos de pessegueiro com base em marcadores codominantes

Contact Info

Product

Resources

About