In vitro culture from mature seeds of Passiflora species

Guzzo, Flavia; Ceoldo, Stefania; Andreetta, Filippo; Levi, Marisa

doi:10.1590/s0103-90162004000100018

Cited by 22 publications

(16 citation statements)

References 10 publications

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“…resultados revelaram que o comportamento in vitro foi diferenciado entre as espécies. Resultados semelhantes foram relatados por GUZZO et al (2004), ao verificarem que embriões de P. apetala, P. candida, P. coriacea, P. subrotunda, P. mayarum e P. morifolia germinaram em meio MS e não houve nenhum sinal de desenvolvimento no meio de cultura B5. ISUTSA (2004), avaliando o estabelecimento e desenvolvimento de P. edulis f. flavicarpa e P. edulis Sims f. edulis em meio de cultura MS verificou que P. edulis f. flavicarpa conseguiu melhor desenvolvimento, enraizamento e maior grau de sobrevivência no processo de micropropagação.…”

Section: Resultsunclassified

Meio de cultura e tipo de explante no estabelecimento in vitro de espécies de maracujazeiro

Faria

Costa

Lêdo³

et al. 2007

Bragantia

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) RESUMOO Brasil é um dos principais centros de dispersão da variabilidade genética do gênero Passiflora. Sua auto-incompatibilidade aliada à incidência de doenças do sistema radicular e da parte aérea, desmatamentos e monocultivos promovem perda de material genético. Tendo-se em vista o risco de erosão genética, torna-se necessário a conservação da variabilidade em bancos de germoplasma, de grande interesse no melhoramento de plantas. Estudos em relação ao tipo de explante e concentração dos meios de cultivo são necessários para se determinar protocolo de estabelecimento e conservação in vitro de germoplasma de maracujá. O objetivo deste trabalho foi avaliar a influência da concentração dos sais e nutrientes do meio MS e tipos de explantes no estabelecimento e crescimento das espécies de maracujazeiro Passiflora giberti N. E. Brown, P. edulis Sims e P. laurifolia L. Em cada espécie de Passiflora havia características próprias quanto ao desenvolvimento in vitro. O meio de cultura MS completo e os segmentos nodais que continham a segunda gema axilar tiveram melhores resultados em relação às demais.Palavras-chave: Passiflora spp., posição da gema, micropropagação. ABSTRACT CULTURE MEDIUM AND TYPE OF EXPLANT IN THE IN VITRO ESTABLISHMENT OF PASSION FRUIT SPECIESBrazil is one of the main centers of genetic variability dispersion of the Passiflora genera. Its self incompatibility as well as disease incidence in its leaves and root system and, deforestation and monocultivation, promote loss of genetic material. Considering the risk of genetic erosion, the conservation of the variability in germplasm banks, which is of great interest in plant breeding, is necessary. Studies regarding the type of explant and concentration of the culture media are necessary in order to determine protocols of establishment and in vitro conservation of passion-fruit germplasm. The objective of the present work was to evaluate the influence of the salt and nutrient concentration in the MS culture medium and types of explants in the establishment and growth of the Passion fruit species: Passiflora giberti N. E.Brown, P. edulis Sims and P. laurifolia L. Each Passiflora species presented its own characteristics regarding in vitro development. The complete MS medium and nodal segments the second axilliary bud promoted better development of the genotypes studied.

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Section: Resultsunclassified

Meio de cultura e tipo de explante no estabelecimento in vitro de espécies de maracujazeiro

Faria

Costa

Lêdo³

et al. 2007

Bragantia

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“…Because of that, this crop is subjected to huge breeding efforts to ensure yield stability and fruit quality (Blat et al, 2005;Crosby, 2008;de Sousa and Maluf, 2003). In this regard, in vitro culture of immature embryos can be useful for shortening generation cycles, producing haploids and double haploids, or rescuing potentially abortive interspecific embryos, which enables the introgression of genes between related species (Bhattarai et al, 2009;Guzzo et al, 2004;Hossain et al, 2003;Lotfi et al, 2003;Yoon et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Growth regulators and darkness increase efficiency in in vitro culture of immature embryos from peppers

Manzur

Calvache-Asensio

Rodríguez‐Burruezo

2014

Sci. agric. (Piracicaba, Braz.)

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Common pepper (Capsicum annuum L.) is one of the most important vegetables in the world, and extensive breeding efforts are being made to develop new improved strains of this species. In this regard, in vitro culture of immature embryos may help breeders accelerate breeding cycles and overcome interspecific barriers, among other applications. In this study, we have optimized a protocol for in vitro culture of immature embryos of C. annuum. Levels of indole-3-acetic acid (IAA) and zeatin have been tested to improve the efficiency (germination rates) of this technique in C. annuum embryos at the four main immature stages (i.e. globular, heart, torpedo, and early cotyledonary) from four varietal types of this species (California Wonder, Piquillo, Guindilla, and Bola). The effect of 5-day initial incubation in the dark was also tested on the most efficient hormone formulation. On average, relatively low levels of both IAA and zeatin

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“…Therefore, this work dealt with inducing the callus and cell culture of P. alata and the investigation of in vitro alkaloid production, with and without L-tryptophan feeding. The cell culture of Passiflora was revised (Drew 1997), and some authors, using several explants such as endosperm and zygotic embryo (Guzzo et al, 2004), internode segments (Biasi et al, 2000) and leaf discs (Monteiro et al, 2000) carried out organogenesis. The culture of isolated protoplasts (naked cells), followed by reproducible plant regeneration, was also studied (Davey et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Search for alkaloids on callus culture of Passiflora alata

Machado

Neto

Salgado

et al. 2010

Braz. arch. biol. technol.

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Preliminary work on Passiflora alata leaves failed to detect harmane alkaloids using LC. The aim of this work was to investigate the production of harmane alkaloids through the cell culture of P. alata, inducing its precursor (Ltryptophan). The leaf explants presented satisfactory results after disinfection, and the callus formation was initiated in MS media with adequate quantities of phytohormones. Sixty days after inoculation, calli were inoculated in the optimized semi-solid MS media, with and without the addition of L-tryptophan (50, 100, 200 mg/L)

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In vitro culture from mature seeds of Passiflora species

Cited by 22 publications

References 10 publications

Meio de cultura e tipo de explante no estabelecimento in vitro de espécies de maracujazeiro

Meio de cultura e tipo de explante no estabelecimento in vitro de espécies de maracujazeiro

Growth regulators and darkness increase efficiency in in vitro culture of immature embryos from peppers

Search for alkaloids on callus culture of Passiflora alata

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