2003
DOI: 10.1590/s0103-90162003000100004
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Abstract: Bacillus thuringiensis is a Gram positive, sporangial bacterium, known for its insecticidal habilities. Survival and conjugation ability of B. thuringiensis strains were investigated; vegetative cells were evaluated in non-sterile soil. Vegetative cells decreased rapidly in number, and after 48 hours the population was predominantly spores. No plasmid transfer was observed in non-sterile soil, probably because the cells died and the remaining cells sporulated quickly. Soil is not a favorable environment for B.… Show more

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Cited by 8 publications
(8 citation statements)
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“…In fact, 75/80 isolates were tested for mobilizing activities using triparental conjugation, but B4–10 was the only one that was positive. These results indicated that there is a potential for plasmid exchanges in the collection of soil isolates, but that their abundances might be scarce, as suggested previously (Thomas et al , 2000, 2001; Ferreira et al , 2003). As a corollary, the conjugation test used may not be representative of the actual transfer occurring in these telluric environments.…”
Section: Discussionsupporting
confidence: 83%
“…In fact, 75/80 isolates were tested for mobilizing activities using triparental conjugation, but B4–10 was the only one that was positive. These results indicated that there is a potential for plasmid exchanges in the collection of soil isolates, but that their abundances might be scarce, as suggested previously (Thomas et al , 2000, 2001; Ferreira et al , 2003). As a corollary, the conjugation test used may not be representative of the actual transfer occurring in these telluric environments.…”
Section: Discussionsupporting
confidence: 83%
“…The NaCl, KCl, and Tween 20 in the PBST buffer solution (pH 7.6) presumably maximized the extraction efficiency by minimizing ionic and hydrophobic interactions between soil particles and the Cry3Bb1 protein (Palm et al 1994). Extraction buffers containing a high content of salts and Tween 20 at a neutral or higher pH have been used in numerous studies (e.g., Hopkins and Gregorich 2003;Ferreira et al 2003;Muchaonyerwa et al 2004;Vadakattu and Watson 2004;Baumgarte and Tebbe 2005;Douville et al 2005) and have resulted in ca. 60-80% recovery of Cry proteins from soil.…”
Section: Elisamentioning
confidence: 99%
“…67,68 Furthermore, the spores of the Bt are sensible to light and susceptible to some very specific pesticides, which is a drawback of the spore-crystal complex for foliar application. 69 However, Bt spores have been isolated from leaves of domestic and wild plants, 70 albeit susceptible to UV irradiation [71][72][73][74][75][76][77] It is still unclear whether these spores were transported by wind, insects or were merely isolated cases. 73 In another study, Ignoffo et.…”
Section: Mode Of Action Of Bt Crystals On Insect Pestsmentioning
confidence: 99%