Growth of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. cell suspension cultures with carbon sources

Mello, Marcia O.; Dias, Carlos Tadeu Santos; Amaral, A F C; Melo, Murilo

doi:10.1590/s0103-90162001000300007

Cited by 20 publications

(23 citation statements)

References 23 publications

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“…It is well known that plant tissues contain and/or utilize different carbohydrates (Mello et al 2001). According to our previous study on the culture of adult plant axillary buds of papaya, the addition of 5.0% sucrose to the induction medium was more effective for the regeneration of shoots than the addition of fructose or maltose.…”

Section: Shoot Regenerationmentioning

confidence: 95%

Production of triploid plants of papaya by endosperm culture

Sun

Liang

et al. 2010

Plant Cell Tiss Organ Cult

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Triploid papaya (Carica papaya) plants were obtained by immature endosperm culture. Visible callusing of the endosperm occurred 21 days after initiation of cultures. A continuously growing callus was observed and a maximum of 68.7% of callus induction frequency was obtained when immature endosperm with embryo was cultured on Murashige and Skoog (MS) medium containing 6.0 lM 2,4-dichlorophenoxyacetic acid (2,4-D), 2.5 lM a-naphthaleneacetic acid (NAA) and 4.0 lM 6-furfurylamino purine (Kn). Shoot buds were produced when the callus was subcultured on a medium supplemented with 6-benzyladenine (BA) or thidiazuron (TDZ) along with NAA. Shoots were detached from the callus and transferred to the elongation medium supplemented with indole-3-acetic acid (IAA) and BA. The combination of 3.0 lM IAA and 1.5 lM BA was the best in terms of the number of cultures (93.8%) showing axillary shoot proliferation. The addition of 2.0 lM indole-3-butyric acid (IBA) to the 1/2 MS medium was most effective at inducing root formation with 90% of the shoots developing four to five roots. Healthy rooted plantlets were transferred to pots containing sterilized bed soil and perlite (3:1) mixture in the greenhouse and 78% of the micropropagated plants survived transplantation. The leaves from endosperm-derived plants showed larger stomata and more chloroplasts in guard cells than that from the parent plants. Over 75% of the endosperm-derived plants were triploid with chromosome number 2n = 3x = 27.

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Section: Shoot Regenerationmentioning

confidence: 95%

Production of triploid plants of papaya by endosperm culture

Sun

Liang

et al. 2010

Plant Cell Tiss Organ Cult

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“…1 In vitro multiplication of Drymaria cordata through direct shoot regeneration. a Shoot bud initiation (arrows) from the cutting edges of a leaves explants in MS medium supplemented with 0.1 mg/l (NAA) and 1.0 mg/l (BA) after 3 weeks in regeneration medium (bar = 0.2 cm); b High frequency regeneration of multiple shoots from the leaves explants in MS medium supplemented with 0.1 mg/l (NAA) and 1.0 mg/l (BA) after 5 weeks in regeneration medium (bar = 1 cm); c Well developed elongated shoots in MS medium after 4 weeks (bar = 1 cm); d Rooting of regenerated shoot on MS supplemented with 1 IBA for 30 days (bar = 1 cm); e A plantlet with well-developed root system (bar = 1 cm); f Acclimatized plantlets growing in a pot containing sterilized bed soil and perlite (3:1) mixture in the green house (bar = 1 cm) It is well known that plant tissues contain and/or utilize different carbohydrates (Mello et al 2001). According to our results, the addition of 3% sucrose to the induction medium was more effective for the regeneration of shoots than the addition of fructose or maltose.…”

Section: Effect Of Carbon Source and The Basal Medium On Organogenesismentioning

confidence: 99%

High-frequency direct shoot regeneration from Drymaria cordata Willd. leaves

Ghimire

Seong

Goh

et al. 2009

Plant Cell Tiss Organ Cult

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An efficient and reproducible procedure is described for direct shoot regeneration in Drymaria cordata Willd. using leaf explants cultured on Murashige and Skoog (MS) medium supplemented with a-naphthalene acetic acid (NAA) and 6-benzyladenine. The regeneration frequency varied with the plant growth regulator concentrations, orientation of the explants, and the carbon source and basal salts present in the regeneration medium. The highest mean number of shoots per explant (10.65 ± 1.03) was recorded on MS plates containing 3% sucrose and 0.8% agar supplemented with 0.1 mg/l NAA and 1.0 mg/l BAP. Shoot buds were induced in the basal parts of the leaf explants. Concentrations of NAA exceeding 1 mg/l suppressed shoot regeneration. Explants bearing the entire lamina and petiole were much more responsive than those having only the lamina. The plantlets that regenerated from the leaf explants were rooted successively on MS medium alone or in combination with indole butyric acid (IBA). The highest mean number of root organogenesis, with 25.67 ± 3.68 roots per leaf segment, was obtained in the presence of 1 mg/l IBA. Histological investigations of the regenerating shoots showed that the shoot buds had emerged from epidermal cells without callus formation. More than 90% of the in vitro-propagated plants survived when transferred to a greenhouse for acclimatization. Thus, this optimized regeneration system may be used for rapid shoot proliferation and genetic transformation.

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“…The zedoary suspension cell culture exhibited growth profiles characterized by a doubling time (T d ) of 5.5 days. This may be attributable to the exhaustion of nutrients or the accumulation of toxic substances in the medium [21]. The dynamic of total protein production is shown in Fig.…”

Section: Plant Cell Suspension Culturementioning

confidence: 99%

Isolation and Characterization of Antioxidation Enzymes from Cells of Zedoary (Curcuma zedoaria Roscoe) Cultured in a 5-l Bioreactor

et al. 2007

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The Multiplex Ligation-dependent Probe Amplification assay (MLPA) is the method of choice for the initial mutation screen in the analysis of a large number of genes where partial or total gene deletion is part of the mutation spectrum. Although MLPA dosage probes are usually designed to bind to normal DNA sequence to identify dosage imbalance, point mutation-specific MLPA probes can also be made. Using the dystrophin gene as a model, we have designed two MLPA probe multiplexes that are specific to a number of commonly listed point mutations in the Leiden dystrophin point mutation database (http://www.dmd.nl). The point mutation probes are designed to work simultaneously with two widely used dystrophin MLPA multiplexes, allowing both full dosage analysis and partial point mutation analysis in a single test. This approach may be adapted for other syndromes with well defined common point mutations or polymorphisms.

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Growth of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. cell suspension cultures with carbon sources

Cited by 20 publications

References 23 publications

Production of triploid plants of papaya by endosperm culture

Production of triploid plants of papaya by endosperm culture

High-frequency direct shoot regeneration from Drymaria cordata Willd. leaves

Isolation and Characterization of Antioxidation Enzymes from Cells of Zedoary (Curcuma zedoaria Roscoe) Cultured in a 5-l Bioreactor

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