PREPARAÇÕES DE Saccharomyces cerevisiae ELICITORAS DE FITOALEXINAS EM MESOCÓTILOS DE SORGO

Wulff, N.A.; Pascholati, Sérgio Florentino

doi:10.1590/s0103-90161998000100022

Cited by 15 publications

(12 citation statements)

References 14 publications

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“…Isolates from S‐ and C‐biotype were obtained from naturally infected shoots (brooms) of Solanum lycocarpum from Tiradentes, Minas Gerais, Brazil (21°11′9·4″S, 44°19′15·8″W) or cacao from Uruçuca, Bahia, Brazil (14°31′7·2″S, 39°18′26·8″W), respectively. Commercial bakers' yeast (Fleischmann) was used as an elicitor (negative control) in a MT inoculation experiment (Wulff & Pascholati, ).…”

Section: Methodsmentioning

confidence: 99%

“…To obtain an elicitor from Saccharomyces cerevisiae , 200 g of a commercial yeast tablet was dissolved in 1 L water (Wulff & Pascholati, ) and autoclaved four times at 121°C for 1 h. Between each autoclaving, cell extracts were chilled on ice–water before the next autoclaving, followed by centrifugation at 15 000 g for 30 min at 4°C. The pellet was then discarded and the supernatant was autoclaved.…”

Section: Methodsmentioning

confidence: 99%

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Interaction of Moniliophthora perniciosa biotypes with Micro‐Tom tomato: a model system to investigate the witches' broom disease of Theobroma cacao

et al. 2014

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The miniature tomato (Solanum lycopersicum) cultivar Micro-Tom (MT) has become an important platform to investigate plant-pathogen interactions. In the case of the witches' broom disease of Theobroma cacao (cacao), the existence of Moniliophthora perniciosa isolates pathogenic to Solanaceae (S-biotype) may enable the use of MT to circumvent limitations of the cacao host, whereas the availability of a non-infective cacao C-biotype allows the evaluation of contrasting responses of MT. Infection of MT by the S-biotype led to stem swelling and axillary shoot growth to form broom-like symptoms similar to the biotrophic phase in cacao, but the infected tissues did not progress to necrosis. Conversely, inoculation with the C-biotype did not cause typical symptoms, but reduced plant height, appearing as a non-host interaction. Histopathological characterization of the S-biotype infection of MT by light and electron microscopy indicated limited germ tube penetration, preferentially through wounds at the base of trichomes or actively through the epidermis. No intracellular mycelium was observed, corroborating the lack of the necrotrophic stage of the pathogen. The analysis of gene expression during the interaction between the S-or C-biotype with MT indicated that expression of plant defence-associated genes differs for kinetics and intensity between a compatible or incompatible M. perniciosa-MT interaction. The pattern of spore germination and low rate of mycelia penetration suggests that the S-biotype is not a fully adapted tomato pathogen, but possibly a case of broken non-host resistance, and evidence suggests the occurrence of a non-host MT response against the C-biotype.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Interaction of Moniliophthora perniciosa biotypes with Micro‐Tom tomato: a model system to investigate the witches' broom disease of Theobroma cacao

et al. 2014

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“…Tubes were kept in moist environment at 27 o C. After 60 h, mesocotyls were weighed, cut in three segments and transferred to tubes containing 80% methanol to extract the phytoalexins. The 3-deoxyanthocyanidins content of the methanolic solution was determined spectrophotometrically at 480 nm (29). For both systems, a regression analysis was performed using the Program SAEG, with concentration as the independent variable (with five levels: 0, 10, 25, 40 and 50%) and absorbance as the dependent variable.…”

Section: Phytoalexin Determinationmentioning

confidence: 99%

Induction of plant defense responses by Ocimum gratissimum L. (Lamiaceae) leaf extracts

Colpas¹,

Schwan-Estrada²,

Stangarlin³

et al. 2009

Summa phytopathol.

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Palavras-chave adicionais: resistance induced sorghum, soybean, cucumber. v.35, n.3, p.191-195, 2009 sistêmica em pepino contra Colletotrichum lagenarium, refletido na redução da incidência da doença e no aumento da produção de quitinase. O modo de ação e a existência de possíveis elicitores de resposta de defesa nos extratos de folhas de O. gratissimum são discutidos. RESUMO ABSTRACTAqueous extracts of the leaves of Ocimum gratissimum at 10, 25, 40 and 50% (w/v) concentrations induced the production of phytoalexins in soybean cotyledons and sorghum mesocotyls. The aqueous extracts also induced systemic resistance in cucumber to Colpas, F.T.; Schwan-Estrada, K.R.F.; Stangarlin, J. R.; Ferrarese, M.L.; Scapim, C.A.; Bonaldo, S.M. Induction of plant defense responses by

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“…Quanto ao comportamento das frações etanólicas, os resultados estão de acordo com Wulff e Pascholati (19,20), pois a fração com maior absorbância (Figura 2) constatada foi a FEI, que também apresentou maior propriedade indutora de fitoalexinas em sorgo diferindo estatisticamente da fração FEII.…”

Section: Cromatografia De Camada Delgada (Cdd)unclassified

Caracterização parcial de frações obtidas de extratos de Cymbopogon nardus com atividade elicitora de fitoalexinas em sorgo e soja e efeito sobre Colletotrichum lagenarium

et al. 2008

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Medic inal plant s present potential to control phytopathogens due to the direct effect or by the activation of defense mechanisms like phytoalexins. Then, the present work had the objective to verify the antifungal and elicitor effect of citronella (Cymbopogon nardus) obtained from methanolic and ethanolic extracts. It was studied the accumulation of phytoalexins in the mesocotyls of sorghum and in cotyledon of soybean, in response to application of fractions from crude extracts methanolic (EME) and ethanolic extracts (EET) of c it ro ne ll a. The EME and EET e xtr ac ts wer e fr ac ti oned by ge l filtration chromatography (CFG) and three fractions were obtained from the methanolic extract (FMI, FMII and FMIII), and two fractions from the ethanolic extract (FEI and FEII). The molecular weight for the FMI, FMII, FMIII, FEI and FEII were 69.29, 40.51, 18.72, 65.89 and 24.11 kDa, respectively v.34, n.4, p.332-337, 2008 Keywords: mesocotyls, cotyledon, glyceolin, deoxyanthocyanidin, citronella.Palavras-chave adicionais: mesocótilos, cotilédones, gliceolina, deoxiantocianidinas, citronela. RESUMOPl antas m edi cinais apr esentam po tenc ial para o co ntr ole de fitopatógenos devido ao efeito direto sobre o agente patogênico ou indireto pela ativação de mecanismos de defesa como as fitoalexinas. Assim, o pres ente trabalho t eve po r obje ti vo ver if ic ar o e fe it o antifúngico e elicitor de frações obtidas da citronela (Cymbopogon nardus), a partir dos extratos brutos metanólico (EME) e etanólico (EET) de ssa pl ant a. Os ext rato s EME e EET for am fracio nados em cromatógrafo de coluna de filtração em gel (CFG) obtendo-se três fraçõe s a par tir do extrato metanólico ( FMI, FM II e FM III) e duas f raç ões para o e xtr ato etanól ico (FEI e FEII). Os pes os moleculares para as fraçõe s FMI, FMII, FMIII, FEI e FEII foram 6 9, 29 ; 40 , 51 ; 18 ,7 2; 65 ,8 9 e 2 4, 11 kDa, r es pe ct ivame nt e. As f r açõ e s o bt i das nas CFG f o r am ut i l izadas e m bi oe ns ai os de ge r m i naç ão de e s po r o s e fo r m aç ão de apr e s só r i o s po r Moreira, C.G.A.; Schwan-Estrada, K.R.F.; Bonaldo, S.M.; Stangarlin, J.R.; Cruz, M.E.S. Caracterização parcial de frações obtidas de extratos de Cymbopogon nardus com atividade elicitora de fitoalexinas em sorgo e soja e efeito sobre Colletotrichum lagenarium Summa Phytopathologica, v.34, n.4, p.332-337, 2008 Col le t ot ri chu m l ag en a ri um e, de i ndução de f it o al e xi nas e m mesocótilos de sorgo e cotilédones de soja. Observou-se que não houve efei to significativo das frações de EME e EET analis adas so br e a ger mi nação e f or mação de apre ssó ri os pel o pat ógeno. Quanto ao acúmul o de fit oale xinas em coti lédones de so ja, não ho uve ef eit o s ignifi cat ivo das fr açõ es FMI, FMII, FMIII, FEI e FEII. Ent re tanto , obse rvo u-se e fe it o s igni fi cati vo das fr aç õe s obtidas na CFG, sobre a produção de fitoalexinas em mesocótilos de s or go , onde o s maio re s ac úm ul os des tas fitoalexinas foram promovidas pelas frações FMI ...

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PREPARAÇÕES DE Saccharomyces cerevisiae ELICITORAS DE FITOALEXINAS EM MESOCÓTILOS DE SORGO

Cited by 15 publications

References 14 publications

Interaction of Moniliophthora perniciosa biotypes with Micro‐Tom tomato: a model system to investigate the witches' broom disease of Theobroma cacao

Interaction of Moniliophthora perniciosa biotypes with Micro‐Tom tomato: a model system to investigate the witches' broom disease of Theobroma cacao

Induction of plant defense responses by Ocimum gratissimum L. (Lamiaceae) leaf extracts

Caracterização parcial de frações obtidas de extratos de Cymbopogon nardus com atividade elicitora de fitoalexinas em sorgo e soja e efeito sobre Colletotrichum lagenarium

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