The objective of this work was to evaluate the yield and physiological quality of seeds of different bean genotypes produced in the off-season period in the subtropical region of Rio Grande do Sul state. The work was divided in two experiments. The first one evaluated the number of pods per plant, number of seeds per plant, number of seeds per pod, thousand seed weight and seed yield per plant of five bean genotypes (IPR Tuiuiú, BRS Embaixador, Guabiju, Carioquinha and Mouro ), during 2013 and 2014 offseason periods. In the second study, the physiological quality of the seeds such as germination, first germination count, shoot and root dry matter were measured under different osmotic potentials (0.0, -0.09, -0.18, and -0,27 MPa) at temperatures of 25 and 35°C. Moreover, the isoenzymatic expression of the seedlings at the temperatures 25 and 35°C, of the seeds from the 2013 crop was evaluated. The results showed that IPR Tuiuiú and Carioca genotypes have superiority in yield components, whereas the BRS Embaixador showed the highest Thousand seed weight. The physiological quality of the seeds was reduced in the lower osmotic potentials at both temperatures. The expression and intensity of isoenzyme peroxidase bands were higher in potencies of -0.09 and -0.18 MPa for all genotypes at both temperatures.