Influência da concentração de NaCl e pH na extração de ricina em torta de mamona (Ricinus communis L.) e sua caracterização por eletroforese

Silva, Bárbara Amorim; Stephan, Marilia Penteado; Koblitz, María Gabriela Bello; Ascheri, José Luis Ramírez

doi:10.1590/s0103-84782012005000042

Cited by 3 publications

(3 citation statements)

References 12 publications

(11 reference statements)

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“…For the best of our knowledge, it is the first time that this method is employed to ricin extraction. This method can be improved for future works, including subsequent steps of protein purification, and comparison with other forms of sample preparation reported in the literature [4,14,32,35]. In addition, the use of ASE combined with SDS-PAGE, MALDI-TOF MS and MALDI-TOF MS/MS, has provided a fast and unambiguous identification method for ricin that can be used in real cases of forensic investigation of suspected samples.…”

Section: Discussionmentioning

confidence: 99%

“…The production of 1.0 ton of oil generates around 1.2 ton of residue, known as castor cake [3]. The literature reports different values for the ricin content in the castor cake, varying between 0.04% and 0.08% (w/w), depending on the cultivars, the extraction method, and the analysis [4,5,6]. Castor cake is an excellent source of nutrients for cattle; however, its content of ricin can intoxicate the animals.…”

Section: Introductionmentioning

confidence: 99%

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A New Method for Extraction and Analysis of Ricin Samples through MALDI-TOF-MS/MS

Sousa¹,

Lima²,

Santos³

et al. 2019

Toxins

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We report for the first time the efficient use of accelerated solvent extraction (ASE) for extraction of ricin to analytical purposes, followed by the combined use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and MALDI-TOF MS/MS method. That has provided a fast and unambiguous method of ricin identification for in real cases of forensic investigation of suspected samples. Additionally, MALDI-TOF MS was applied to characterize the presence and the toxic activity of ricin in irradiated samples. Samples containing ricin were subjected to ASE, irradiated with different dosages of gamma radiation, and analyzed by MALDI-TOF MS/MS for verification of the intact protein signal. For identification purposes, samples were previously subjected to SDS-PAGE, for purification and separation of the chains, followed by digestion with trypsin, and analysis by MALDI-TOF MS/MS. The results were confirmed by verification of the amino acid sequences of some selected peptides by MALDI-TOF MS/MS. The samples residual toxic activity was evaluated through incubation with a DNA substrate, to simulate the attack by ricin, followed by MALDI-TOF MS/MS analyses.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A New Method for Extraction and Analysis of Ricin Samples through MALDI-TOF-MS/MS

Sousa¹,

Lima²,

Santos³

et al. 2019

Toxins

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“…A mamoneira (Ricinus communis L.) é uma oleaginosa que tem alto valor econômico, pois apresenta um mercado bem definido para o óleo extraído de suas sementes. Atualmente um grande interesse tem sido dado à torta, por seu alto teor de proteínas (SCHIELTZ et al, 2011;SILVA et al, 2012). O desenvolvimento de estudos que abordam resíduos agrícolas não comestíveis, tem possibilitado a produção de vários materiais de embalagens agrícolas renováveis e biodegradáveis.…”

Section: Introductionunclassified

Desenvolvimento de filmes biodegradáveis à base de proteína da torta de mamona (Ricinus communis L.) modificada com glioxal e reforçados com fibras de celulose

Oliveira¹

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Agradeço a Deus por estar sempre ao meu lado abrindo meus caminhos. A meus pais João e Francisca, por serem meus maiores exemplos, por sempre me apoiarem e pelo amor incondicional que me oferecem. A minha irmã Latulia, pela cumplicidade, amizade, risadas e apoio. Ao meu orientador Prof. Sobral, pelo seu exemplo como pesquisador, pela dedicação, paciência, incentivo e confiança. A Faculdade de Zootecnia e Engenharia de Alimentos (FZEA/USP) pela oportunidade de realização do curso. A Gisele Lourenço e Noemi Chambi, por todas as vezes que me auxiliaram com o projeto e nunca se negarem a tirar minhas dúvidas. Muito Obrigado.

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Cellulose fiber reinforced biodegradable films based on proteins extracted from castor bean (Ricinus communis L.) cake

Oliveira¹,

Makishi²,

Chambi³

et al. 2015

Industrial Crops and Products

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Influência da concentração de NaCl e pH na extração de ricina em torta de mamona (Ricinus communis L.) e sua caracterização por eletroforese

Abstract: (4,0; 4,6; 6,0; 7,4; 8,0)

Cited by 3 publications

References 12 publications

A New Method for Extraction and Analysis of Ricin Samples through MALDI-TOF-MS/MS

A New Method for Extraction and Analysis of Ricin Samples through MALDI-TOF-MS/MS

Desenvolvimento de filmes biodegradáveis à base de proteína da torta de mamona (Ricinus communis L.) modificada com glioxal e reforçados com fibras de celulose

Cellulose fiber reinforced biodegradable films based on proteins extracted from castor bean (Ricinus communis L.) cake

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