Atividade antimicrobiana de óleos essenciais de condimentos frente a Staphylococcus spp isolados de mastite caprina

Pozzo, Marcelo Dal; Viégas, Julio; Santurio, Deise Flores; Rossatto, Luana; Soares, Isaura Helena; Alves, Sydney Hartz; Costa, Mateus Matiuzzi da

doi:10.1590/s0103-84782011005000029

Cited by 19 publications

(18 citation statements)

References 17 publications

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“…However, antimicrobial activity (6,4 mg essential oil : 1 ml methanol) was not observed against Staphylococcus aureus in recent study (Pozzo et al 2011).…”

Section: Introductionmentioning

confidence: 85%

Chemical composition of sage (Salvia officinalis L.) essential oil from the Rio de Janeiro State (Brazil)

Porte

Godoy

Maia-Porte

2013

Rev. bras. plantas med.

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ABSTRACT:The purpose of this study was to investigate the chemical composition of the essential oil from fresh leaves of sage (Salvia officinalis L.) from Petrópolis, Rio de Janeiro State, for international trade. The oil was isolated by hydrodistillation in a Clevenger-type apparatus and analyzed through a combination of GC-FID and GC-MS. The yield was 2.3 % on dry basis. Forty-seven constituents were identified according to their chromatographic retention indices and mass spectra, corresponding to 94.90 % of the compounds present. The major constituents of the oil were α-thujone (40.90 %), camphor (26.12 %), α-pinene (5.85 %) and β-thujone (5.62 %). The essential oil studied was similar to those found in several European countries and can be a valuable product for the small farmers from the Petrópolis region in Rio de Janeiro State.Keywords: Lamiaceae, Salvia officinalis, essential oil RESUMO: Composição química do óleo essencial de Salvia (Salvia officinalis) do Estado do Rio de Janeiro (Brasil). O objetivo deste estudo foi investigar a composição química do óleo essencial de folhas de sálvia (Salvia officinalis L.) de Petrópolis, Rio de Janeiro, para o comércio internacional. O óleo foi isolado por arraste a vapor em aparelho de Clevenger e analisado por CG-DIC e CG-EM. O rendimento foi de 2,3 % em base seca. Quarenta e sete substâncias foram identificadas de acordo com seus índices de retenção e espectros de massas, correspondendo a 94,90% dos compostos presentes no óleo. Os principais constituintes do óleo foram α-tujona (40,90 %), cânfora (26,12 %), α-pineno (5,85 %) e β-pineno (5,62 %). O óleo estudado foi similar àqueles encontrados em vários países europeus e pode ser um valioso produto para pequenos produtores da região de Petrópolis no Estado do Rio de Janeiro. Palavras

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“…However, antimicrobial activity (6,4 mg essential oil : 1 ml methanol) was not observed against Staphylococcus aureus in recent study (Pozzo et al 2011).…”

Section: Introductionmentioning

confidence: 85%

Chemical composition of sage (Salvia officinalis L.) essential oil from the Rio de Janeiro State (Brazil)

Porte

Godoy

Maia-Porte

2013

Rev. bras. plantas med.

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“…glabrata isolates concentration-dependently, with the MICs ranging from 0.5 to 1100 μg/mL (mean: 340.2 μg/mL) for oregano. Dal Pozzo et al [46] studied the antimicrobial activities of 7 spice EOs, and some majority constituents of these spices such as carvacrol, thymol, cinnamaldehyde, and cineole against 33 Staphylococcus spp. isolates from herds of dairy goats, by determining the MICs and MBCs using the broth microdilution method.…”

Section: Oreganomentioning

confidence: 99%

Antibacterial and Antifungal Activities of Spices

Liu

Meng

Wang

et al. 2017

IJMS

272

174

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Infectious diseases caused by pathogens and food poisoning caused by spoilage microorganisms are threatening human health all over the world. The efficacies of some antimicrobial agents, which are currently used to extend shelf-life and increase the safety of food products in food industry and to inhibit disease-causing microorganisms in medicine, have been weakened by microbial resistance. Therefore, new antimicrobial agents that could overcome this resistance need to be discovered. Many spices—such as clove, oregano, thyme, cinnamon, and cumin—possessed significant antibacterial and antifungal activities against food spoilage bacteria like Bacillus subtilis and Pseudomonas fluorescens, pathogens like Staphylococcus aureus and Vibrio parahaemolyticus, harmful fungi like Aspergillus flavus, even antibiotic resistant microorganisms such as methicillin resistant Staphylococcus aureus. Therefore, spices have a great potential to be developed as new and safe antimicrobial agents. This review summarizes scientific studies on the antibacterial and antifungal activities of several spices and their derivatives.

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“…O Tomilho, também utilizado indústria alimentícia, apresenta significativo potencial antimicrobiano devido à presença de seu constituinte Timol (Pozzo et al, 2011). Já o Cinnamomum cassia (canela) possui atividade antimicrobiana devido à presença do aldeído cinâmico .…”

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Atividade antibacteriana de Óleos Essenciais sobre Streptococcus mutans e Staphylococcus aureus

Freire

Perez

Cardoso

et al. 2014

Rev. bras. plantas med.

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RESUMO:Este trabalho objetivou avaliar a ação antimicrobiana in vitro dos óleos essenciais de Ocimum basilicum (Manjericão Exótico), Thymus vulgaris (Tomilho Branco), e de Cinnamomum cassia (Canela da China) sobre cepas bacterianas de Streptococcus mutans (ATCC 25175) e Staphylococcus aureus (ATCC 25923). A atividade antibacteriana dos óleos essenciais foi determinada pela Concentração Inibitória Mínima (CIM) e a Concentração Bactericida Mínima (CBM) através da técnica de microdiluição e do esgotamento. Para a CIM, foram utilizadas placas de 96 poços e inseriu-se 100µL de caldo BHI, 100µL da diluição dos óleos essenciais no primeiro poço e 10µL da suspensão bacteriana (1,5x10 6 microrganismos/ mL). Realizou-se a diluição seriada partindo-se da concentração inicial de 8% até 0,0625%. A CIM correspondeu à última diluição na qual não foi verificada a presença de bactérias. Para obter a CBM, realizou-se a semeadura em Ágar Miller-Hinton das diluições correspondentes a CIM, 2CIM e 4CIM. As placas foram incubadas a 37º C em estufa bacteriológica por 24 horas. Os testes foram realizados em triplicata tendo a clorexidina como controle positivo. Para S. aureus (ATCC 25923) a CIM e CBM dos óleos essenciais de C. cassia, O. basilicum e T. vulgaris foram 0,0625%, 4% e 0,0625%, respectivamente. Para S. mutans a CIM e a CBM dos óleos essenciais de C. cassia e T. vulgaris foram 0,125% e 0,25%, respectivamente. Já a CIM do O. basilicum foi 4% e não apresentou ação bactericida. Conclui-se que os óleos essenciais avaliados apresentaram ação antibacteriana frente a cepas de S. mutans e S. aureus, sendo que os menores valores de CIM e de CBM foram provenientes dos óleos de C. cassia e T. vulgaris. Palavras-chave: Streptococcus mutans, Staphylococcus aureus, Microbiologia. ABSTRACT: Antibacterial Activity of Essential Oils against Strains of Streptococcus and Staphylococcus:The aim of this study is to evaluate the in vitro antimicrobial activity of essential oils of Ocimum basilicum (basil), Thymus vulgaris (thyme) and Cinnamomum cassia (Chinese cinnamon) against strains of Streptococcus mutans (ATCC 25175) and Staphylococcus aureus (ATCC 25923). The antibacterial activity of the essential oils was determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The MIC was obtained by the microdilution technique using 96-well microplates, in which we inserted: 100µL of Brain Heart Infusion broth at double concentration, 100µL of the dilution of the essential oils and 10µL of bacterial suspension (1.5 x 10 6 organisms / mL). The products were diluted from the initial concentration of 8% up to 0.0625%. The MIC corresponded to the last dilution in which there was no presence of bacteria or turbidity in the culture medium. The MBC was obtained by seeding, in Mueller-Hinton agar, 10µL aliquots of dilutions corresponding to the MIC and the two immediately preceding ones (2MIC and 4MIC). The plates were incubated at 37°C in a bacteriological incubator for 24 hours. The tests were performed in triplicate, ...

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Atividade antimicrobiana de óleos essenciais de condimentos frente a Staphylococcus spp isolados de mastite caprina

Abstract: RESUMO Avaliou-se a atividade antimicrobiana dos óleos essenciais (OEs) de Origanum vulgare (orégano), Thym us vulgaris (tomilho), Lippia graveolens (lípia),

Cited by 19 publications

References 17 publications

Chemical composition of sage (Salvia officinalis L.) essential oil from the Rio de Janeiro State (Brazil)

Chemical composition of sage (Salvia officinalis L.) essential oil from the Rio de Janeiro State (Brazil)

Antibacterial and Antifungal Activities of Spices

Atividade antibacteriana de Óleos Essenciais sobre Streptococcus mutans e Staphylococcus aureus

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