Determinação sérica de haptoglobina, ceruloplasmina, α1-glicoproteína ácida, transferrina e α1-antitripsina, em equinos com cólica

Filippo, Paula Alessandra Di; Nogueira, Andressa Francisca da Silva; Santana, Áureo Evangelista

doi:10.1590/s0103-84782011001200012

Cited by 10 publications

(5 citation statements)

References 6 publications

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“…Estos resultados si bien no son comparables con otros estudios en que se utiliza técnicas y condiciones analíticas distintas, presentaron menor coeficiente de variación que el observado en equinos sanos (~31-33%), (11,12) o con alteraciones intestinales (67%), (11); sin embargo, superior a los resultados observados en ponis (4.9%), (13). Por otra parte, cuando se compara los valores obtenidos (Tabla 1) con los reportados en potrillos con una técnica similar, los cuales fueron inferiores (0.57 ± 0.17 DDO), (14).…”

Section: Resultsunclassified

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Asociaciones entre las concentraciones de cobre sérico, cobre hepático y ceruloplasmina sérica en equinos

Noro

et al. 2013

Rev MVZ Córdoba

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RESUMENObjetivo. Determinar los valores y el grado de asociación entre las concentraciones de cobre (Cu) hepático y sérico y de ceruloplasmina (Cp) sérica en equinos. Materiales y métodos. Se utilizaron 38 equinos adultos, beneficiados en plantas faenadoras de la Región de la Araucanía, Chile. Se obtuvieron muestras de sangre por venopunción yugular previo al beneficio para determinar las concentraciones séricas de Cu y de Cp y, de hígado posterior al beneficio para determinar la concentración de Cu. Se calculó los intervalos de referencia y la asociación entre las concentraciones de Cu hepático y sérico y Cp sérica mediante correlación de Pearson y regresión lineal. Resultados. Se estimaron intervalos para las concentraciones de Cu hepático (32 - 161 μmol/kg de materia húmeda) y sérico (10 – 24 μmol/L) y, para Cp sérica (0.08 - 0.43 ΔDO). Los valores de Cu hepático, al ser comparados con los reportados por la literatura, indican que los animales presentaron un nivel adecuado de Cu, a excepción de 1 caballo, que se encontró bajo el límite inferior. La cupremia se asoció con la concentración sérica de Cp (r=0.49; p<0.05), no apreciándose asociación entre las otras variables (p>0.05). Conclusiones. Las concentraciones de Cu hepático de equinos adultos, beneficiados en plantas faenadoras de la Región de la Araucanía, Chile, se encontraron dentro de los intervalos descritos en la literatura. Las concentraciones de Cu hepático no se asociaron con las de Cu o Cp séricas.

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Section: Resultsunclassified

“…Además, animales que cursan con procesos inflamatorios incrementan la concentración sérica de Cp por ser una proteína de fase aguda positiva (11). Los animales del presente estudio eran mestizos, adultos, de buena condición corporal no presentaban signos clínicos ni posmortem de enfermedad.…”

Section: Resultsunclassified

Asociaciones entre las concentraciones de cobre sérico, cobre hepático y ceruloplasmina sérica en equinos

Noro

et al. 2013

Rev MVZ Córdoba

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“…Often these proteins increase because of activation of the inflammatory response to regulate the different phases of inflammation [ 36 , 37 ]. It is possible that ceruloplasmin, albumin, IgA, IgG, transferrin, and haptoglobin are the acute-phase proteins most responsive to abdominal insults such as peritoneal inflammation and infection promoted by the cecal trocarization [ 38 – 41 ], since the horses in the control group showed no changes in acute-phase proteins in plasma and peritoneal fluid over time. Similarly, other studies have shown an increase in fibrinogen and haptoglobin in the peritoneal fluid of horses with peritonitis [ 12 , 27 , 40 , 42 – 45 ].…”

Section: Discussionmentioning

confidence: 99%

Complications following transcutaneous cecal trocarization in horses with a cattle trocar and a cecal needle

Dória

Reginato²,

Hayasaka³

et al. 2022

PLoS ONE

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Percutaneous decompression of the cecum is a procedure that could be considered for horses with cecal gas distension. The aim of this study was to identify complications such as peritonitis and clinically relevant peritonitis (CRP) after transabdominal cecal trocarization in healthy horses using a cattle trocar and a cecal needle. Mixed breed horses were assigned to three groups (n = 6): horses that underwent trocarization with a cecal needle (G1) or a cattle trocar (G2), and a control group (CG) without cecal trocarization. The same horses were used in each group, respecting a three-month washout period between studies. A physical examination, serial blood, and peritoneal fluid sampling were performed, prior to cecal trocarization and 2, 6 and 12 hours after the first collection and 1, 2, 3, 7, and 14 days after the procedure. Acute-phase proteins in blood and peritoneal fluid were analyzed by polyacrylamide gel electrophoresis. Horses with a high cell count in the peritoneal fluid (i.e., 10,000 cells/μl) were considered to have peritonitis and CRP if they met at least two of the following clinical criteria: anorexia, lethargy, tachycardia, tachypnea, fever, ileus, abnormal oral mucous membrane color, abnormal white blood cells count, or high blood fibrinogen concentration (> 5 g/L). All horses recovered from cecal trocarization and abdominocentesis with no major complications. Cecal trocarization caused cytologic evidence of peritonitis in G1 and G2 during the 14 days of evaluation. CRP was not observed, although a decrease in cecal motility was observed in G1 and G2 during the experimental period and three horses, one from G1 and two from G2, showed a single moment of fever. None of the groups showed leukopenia or leukocytosis, although blood neutrophil count decreased at D7 and D14 in G1 and at D14 in G2 (p ≤ 0.05). After cecal trocarization, an increase in the total nucleated cells count, total proteins, globulins, alkaline phosphatase and acute phase proteins were observed in the peritoneal fluid of G1 and G2 during the 14 days of evaluation (p ≤ 0.05), without causing clinically relevant peritonitis. Transcutaneous cecal trocarization promotes peritonitis, which is more intense with a cattle trocar than with a cecal needle. The cecal needle should be considered for cecal trocarization of horses with cecal tympany.

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“…Nevertheless, IgA appears to be related to an inflammatory stimulation, acting as a second line of defense in the elimination of pathogens that have penetrated through the surface mucous barrier (Snoeck et al 2006). IgA can also act as a secretory antibody within the intestinal tract and lungs, able to neutralize viruses and prevent adherence of bacterial pathogens to target tissues (Di Filippo et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Acute phase proteins are primarily produced in the liver (Murata et al 2004, Petersen et al 2004, Ceron et al 2005. However, extrahepatic production of APPs is also possible in most mammalian species (Jacobsen et al 2006, Di Filippo et al 2011, 2014a. Some APPs opsonize microorganisms and activate complements, while others scavenge cellular remnants and free radicals or neutralize proteolytic enzymes (Gruys et al 2005).…”

Section: Introdutionmentioning

confidence: 99%

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

et al. 2018

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Use of acute-phase proteins (APPs) for assessment of health and disease in animals has increased greatly within the last decade. The objective was to determine the normal concentration of APPs in the serum and cerebrospinal fluid (CSF) of healthy cattle by polyacrylamide gel electrophoresis. Fifty crossbred animals (350±70kg of BW and 18±1.2 months of age), 25 heifers and 25 steers were used. CSF samples were collected from atlanto-occipital (AO) site and blood samples were obtained from the jugular vein. CSF and serum protein electrophoresis were performed by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Thirty-seven proteins with molecular weights ranging from 7 and 37kDa were identified in CSF of all animals. These eight were nominally identified with immunoglobulin A and G, celuloplasmin, transferrin, albumin, α1-antitripsin, acidic glycoprotein, and haptoglobin. All protein fractions in CSF did not differ between heifers and steers. In sera, 34 proteins with molecular weights between 7 and 244kDa were identified in heifers and steers. Similar proteins were nominally identified in the sera, but only the CSF presented α1-antitripsin. The serum values of acidic glycoprotein and immunoglobulin G were significantly higher in steers compared with heifers. In conclusion, measurement of CSF acute phase protein concentrations can be useful in diagnosing and monitoring the progression of bovine neurological diseases, perhaps even to guide therapeutic procedures. The CSF electrophoretic profile of healthy cattle does not change depending on gender.

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Determinação sérica de haptoglobina, ceruloplasmina, α1-glicoproteína ácida, transferrina e α1-antitripsina, em equinos com cólica

Abstract: RESUMO Foram examinados 46 equinos adultos, 6 hígidos (G1) e 40 com cólica, submetidos à laparotomia. Vinte apresentavam lesões no intestino grosso (G2) e 20 no intestino delgado (G3

Cited by 10 publications

References 6 publications

Asociaciones entre las concentraciones de cobre sérico, cobre hepático y ceruloplasmina sérica en equinos

Asociaciones entre las concentraciones de cobre sérico, cobre hepático y ceruloplasmina sérica en equinos

Complications following transcutaneous cecal trocarization in horses with a cattle trocar and a cecal needle

Acute phase proteins in serum and cerebrospinal fluid in healthy cattle: possible use for assessment of neurological diseases

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