Espermatozoides caprinos criopreservados em meio à base de leite desnatado acrescido de glutationa reduzida

Abstract

RESUMO Visando avaliar o efeito da adição de glutationa reduzida (GSH) ao diluente de congelação de sêmen caprino à base d e leite d esn ata do INTRODUÇÃOA redução da fertilidade, associada à inseminação artificial com sêmen congelado, é atribuída à ocorrência de danos espermáticos durante a congelação e descongelação, causando alterações ultraestruturais, bioquímicas e funcionais que reduzem a viabilidade espermática. Estudos têm relatado que muitos mecanismos de defesa antioxidante estão envolvidos nos sistemas biológicos e que o equilíbrio

Glutathione and IGF-1 in bovine seminal cryopreservation: oxidative stress response and pregnancy rate

“…The same was noted by Stradaioli et al (2007), who observed that in the extenders for bovine egg yolks there is a decrease in the concentration of gSH when compared with those without products of animal origin. As was found by Soares et al (2011), noting that the different concentrations were not able to preserve membrane integrity. Sariozkan et al (2009), when working with Holstein cattle, added to the 2mM diluter of gSH and observed the maintenance of the integrity of the spermatic membranes when compared with the control group.…”

“…In the present study, it is noted that the percentage of cells with the intact plasma membrane in all treatments was low, as well as that of the undamaged acrosome, but this response may be associated with increased fluorescence of the probes used by the TRIS diluter. However, due to the low response of the sperm membranes, it should be noted that the spermatozoids with intact plasmatic and acrosomal membranes are more apt to perform fertilization (Soares et al 2011).…”