NPY and sbGnRH gene expression in juvenile and adult male Brazilian flounder Paralichthys orbignyanus

Campos, Vinícius Farias; Collares, Tiago; Seixas, Fabiana Kömmling; Deschamps, João Carlos; Marins, Luís Fernando; Okamoto, Marcelo Hideo; Sampaio, Luís André; Robaldo, Ricardo Berteaux

doi:10.1590/s0103-84782011001100013

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…For real-time polymerase chain reaction (PCR), cells were seeded in six-well flat bottom plates at a density of 2 × 10 5 cells per well and grown at 37 °C in a 5% CO 2 atmosphere for 24 h. Cells were then incubated with 100 or 50 μM of zidovudine, 3a , 3b , or 3h for 48 h. After this period, cells were washed with phosphate-buffered saline (Gibco), and RNA extraction was performed. Total RNA extraction, cDNA synthesis, and real-time PCR were conducted as previously described. , Briefly, RNA samples were isolated using TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and samples were DNase-treated with a DNA-free kit (Ambion) following the manufacturer’s protocol. First-strand cDNA synthesis was performed with 2 μg of RNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer’s protocol.…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA extraction, cDNA synthesis, and real-time PCR were conducted as previously described. 41,42 Briefly, RNA samples were isolated using TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and samples were DNase-treated with a DNA-free kit (Ambion) following the manufacturer's protocol. First-strand cDNA synthesis was performed with 2 μg of RNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer's protocol.…”

Section: ■ Associated Contentmentioning

confidence: 99%

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New Organochalcogen Multitarget Drug: Synthesis and Antioxidant and Antitumoral Activities of Chalcogenozidovudine Derivatives

et al. 2015

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In this article we present the synthesis, characterization, and in vitro biological and biochemical activities of new chalcogenozidovudine derivatives as antioxidant (inhibition of TBARS in brain membranes and thiol peroxidase-like activity) as well as antitumoral agents in bladder carcinoma 5637. A prominent response was obtained for the selected chalcogenonucleosides, showing effective antioxidant and antitumoral activities.

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Section: Methodsmentioning

confidence: 99%

Section: ■ Associated Contentmentioning

confidence: 99%

New Organochalcogen Multitarget Drug: Synthesis and Antioxidant and Antitumoral Activities of Chalcogenozidovudine Derivatives

et al. 2015

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“…Giving respective examples, Npy stimulated the release of gonadotropins and gonadotropinreleasing hormone (GnRH) in several fish species [135]. Npy mRNA expression in brain has been found to be twice as high in adult Brazilian flounder (Paralichthys orbignyanus) than that of the juvenile fish [136], signifying that Npy may have a significant role in the sexual growth and reproductive procedures in fish. On the other hand, a noteworthy decrease in feeding was exposed in seabass (Dicentrarchus labrax) after they were treated with testosterone and estrogen implants [137].…”

Section: Key Recognized Appetite-regulating Endocrine Factors In Teleostmentioning

confidence: 99%

The Roles of Neuropeptide Y (Npy) and Peptide YY (Pyy) in Teleost Food Intake: A Mini Review

Assan

Mustapha

Chen

et al. 2021

Life

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Neuropeptide Y family (NPY) is a potent orexigenic peptide and pancreatic polypeptide family comprising neuropeptide Y (Npy), peptide YYa (Pyya), and peptide YYb (Pyyb), which was previously known as peptide Y (PY), and tetrapod pancreatic polypeptide (PP), but has not been exhaustively documented in fish. Nonetheless, Npy and Pyy to date have been the key focus of countless research studies categorizing their copious characteristics in the body, which, among other things, include the mechanism of feeding behavior, cortical neural activity, heart activity, and the regulation of emotions in teleost. In this review, we focused on the role of neuropeptide Y gene (Npy) and peptide YY gene (Pyy) in teleost food intake. Feeding is essential in fish to ensure growth and perpetuation, being indispensable in the aquaculture settings where growth is prioritized. Therefore, a better understanding of the roles of these genes in food intake in teleost could help determine their feeding regime, regulation, growth, and development, which will possibly be fundamental in fish culture.

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Neuropeptide Y gene expression around meal time in the Brazilian flounder Paralichthys orbignyanus

Campos¹,

Robaldo

Deschamps³

et al. 2012

J Biosci

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Neuropeptide Y (NPY) is considered the major stimulant for food intake in mammals and fish. Previous results indicate that NPY is involved in the feeding behaviour of the Brazilian flounder, Paralichthys orbignyanus. In this study, we evaluated hypothalamic NPY expression before (-2 h), during (0 h) and after feeding (+2 h) in two independent experiments: (1) during a normal feeding schedule and (2) in fish fasted for 2 weeks. During normal feeding, changes in the levels of NPY mRNA were periprandial, with expression levels being significantly elevated at meal time (P less than 0.05) and significantly reduced 2 h later (P less than 0.05). Comparing the fasting and unfasted groups, NPY mRNA levels were significantly higher (P less than 0.05) at -2 h and +2 h in the fasting group, but there was no difference at 0 h. In addition, the higher NPY mRNA levels that were observed in the fasting group were maintained throughout the sampling period. In summary, our results show that NPY expression was associated with meal time (0 h) in food intake regulation.

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NPY and sbGnRH gene expression in juvenile and adult male Brazilian flounder Paralichthys orbignyanus

Abstract: ABSTRACT

Cited by 6 publications

References 17 publications

New Organochalcogen Multitarget Drug: Synthesis and Antioxidant and Antitumoral Activities of Chalcogenozidovudine Derivatives

New Organochalcogen Multitarget Drug: Synthesis and Antioxidant and Antitumoral Activities of Chalcogenozidovudine Derivatives

The Roles of Neuropeptide Y (Npy) and Peptide YY (Pyy) in Teleost Food Intake: A Mini Review

Neuropeptide Y gene expression around meal time in the Brazilian flounder Paralichthys orbignyanus

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