Diagnóstico e genotipagem do vírus da pseudoraiva por nested-PCR e análise de restrição enzimática

Júnior, Antônio Augusto Fonseca; Carmagos, Marcelo Fernandes; Dambros, Régia Maria Feltrin; Braga, Alexandre C.; Zanella, J. R. C.; Heinemann, Marcos Bryan; Leite, Rômulo Cerqueira; Reis, Jenner Karlisson Pimenta dos

doi:10.1590/s0103-84782010005000066

Cited by 9 publications

(3 citation statements)

References 19 publications

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“…Virus isolates used in this research were the standard strain Shope, vaccine strain Bartha and fourteen Brazilian SuHV-1 isolates (four genotype I and ten genotype II) previously characterized by FONSECA JR. et al (2010a) andFONSECA JR et al (2012). Viruses were grown in PK15 cells and DNA extracted as described by FONSECA JR et al (2010b) (Group 1).…”

Section: Methodsmentioning

confidence: 99%

Different methods of real-time PCR for detection of pseudorabies virus

et al. 2017

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Pseudorabies (PR) is a highly contagious viral disease of great animal health and economic importance in swine industry. The aim of this study was to evaluate different genomic regions, real-time PCR chemistries and equipment for the molecular diagnosis of PR. Eight primer pairs targeting four genes (gB, gC, gE, gD), three different qPCR chemistries (SybrGreen, hydrolysis probes and plexor) and two equipment (ABI7500, Rotorgene 3000) were evaluated. Oligonucleotides targeting gB using hydrolysis probes showed the best performance after evaluating efficiency (99%), the detection limit (10-1.5 TCID50 mL-1) and diagnostic sensitivity and; therefore, those primers were selected for performance verification factors such as repeatability, reproducibility and robustness (1.39% variance between days, 24% variance between analysts and 4.07% variance in analysis error). The qPCR standardized and validated in this research proved to be reliable for the diagnosis of PR and may be used in diagnostic laboratories that follow ISO 17025 and ISO 16140.

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Section: Methodsmentioning

confidence: 99%

Different methods of real-time PCR for detection of pseudorabies virus

et al. 2017

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“…The swine herpesvirus 1 (SuHV-1) strain NP (Nova Prata) (Fonseca et al, 2010) was propagated in MDBK (Mardin and Darby Bovine Kidney -ATCC CCL 22). All cells were grown in MEM with 10% fetal bovine serum (FBS) (Simoni et al, 2007).…”

Section: Cell Cultures and Virusesmentioning

confidence: 99%

In vitro antiviral activity of Brazilian Cerrado plant extracts against animal and human herpesviruses

Padilla¹,

Simoni²,

Hoe³

et al. 2018

J. Med. Plants Res.

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The Brazilian savanna known as "Cerrado" is very rich in medicinal plants that are used by the local population for treatment of several illnesses. The herpesvirus is a serious problem worldwide, and affects both animal and human health. This work aimed to study the antiviral activity of eight extracts from plants natives of "Cerrado" region against human (HSV-1), equine (EqHV-1) and swine herpesviruses (SuHV-1). The results showed that all plant extracts: Banisteriopsis variabilis, Byrsonima intermedia, Campomanesia xanthocarpa, Erythroxilum deciduum, Lacistema hasslerianum, Ocotea pulchella, Stryphodendron adstringens and Xylopia aromatica presented antiviral activity against at least one herpesvirus. Furthermore, it was observed a direct anti-herpes effect of extracts from B. variabilis and B. intermedia in non-toxic concentrations against all herpesviruses. B. intermedia crude aqueous extract showed the most promising results with selective index values of the 41.76 ± 0.04; 4.12 ± 0.1 and 193.97 ± 0.09 respectively against HSV-1, EqHV-1 and SuHV-1. Due to this, B. intermedia extract was also analyzed by HPLC/MS allowing for the identification of gallic acid and quercetin as main compounds.

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“…As amostras virais utilizadas foram herpesvírus bovino (BoHV-1), cepa Los Angeles (Straub, 2001) e herpesvírus suíno (SuHV-1), cepa Nova Prata gentilmente cedida pelo Instituto Desidério Finamor, RS, Brasil (Fonseca Junior et al, 2010).…”

Section: Vírus E Célulasunclassified