Overlapping of mononuclear cells derived from bone marrow in rats' intervertebral discs: an in vitro study

Fontes, Érica Batista; Borges, Andréa Pacheco Batista; Pippi, Ney Luís; Rosa, Maurício Borges da; Sprada, Arícia Gomes; Togni, Monique

doi:10.1590/s0103-84782010005000042

Cited by 1 publication

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References 17 publications

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“…Isolation of MNCs were done according to Fontes et al [12] After removing all BM in 3ml PBS containing 10% FBS, it was centrifuged for 5 minutes at 160 g. The supernatant was removed and the precipitated fraction was resuspended in 1ml of PBS with 10% fetal bovine serum (FBS). The suspension was added over 0.5ml of Ficoll-Paque.…”

Section: Preparation Of Mononuclear Cell (Mnc) Suspension From Bm Plugmentioning

confidence: 99%

Autologous-Versus Allogeneic-Bone Marrow Cell Grafting in Prevention of Obstructive Nephropathy in Rats

Fattah

Badawi

Mohamed

2019

Egyptian Journal of Histology

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Background: The increasing number of patients suffering from chronic renal disease (CRD) and end-stage renal disease (ESRD) are a challenge for the development of ingenious therapies. Bone marrow (BM) stem cells are an attractive therapy in the treatment of CRD. Aim of the work: This study aims to compare autologous and allogeneic BM cells in treatment of renal damage in a model of unilateral ureteral obstruction (UUO). Materials and methods: Sixty adult female Sprague-Dawley rats were divided randomly into four groups of 15 animals each: Rats of first group served as (Sham group). The sham-operated animals underwent identical surgical procedures, except that the left ureter was manipulated without ligation and sectioning. Rats underwent UUO with direct intra-renal injection of saline were considered as (UUO) group. The remaining rats were injected with autologous (Auto group) or allogeneic (Allo group) BM cells directly into the renal parenchyma at the same day of operation. In Allo group, the ages of the donor rats were approximately the same as the recipient rats. Results: In the present study, we showed that both types BM cells provided a protective effect and ameliorated interstitial fibrosis as reflected by reduced tubular dilation, decreased interstitial expression of .-smooth muscle actin and significant decline in expression of both transforming growth factor-.1 and caspase-3 in the tubular cells. Autologous BM cells reduced the tubular dilation index from 36.6 (as in UUO rats) to 8.13. Allogeneic BM cells reduced tubular dilation index to 16.87. Conclusion: Our results show that a single intra-renal administration of autologous or allogeneic BM cells strongly associated with a reno-protective role with enhanced proliferation and reduced apoptosis of tubular cells promoted by UUO.

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Section: Preparation Of Mononuclear Cell (Mnc) Suspension From Bm Plugmentioning

confidence: 99%

Autologous-Versus Allogeneic-Bone Marrow Cell Grafting in Prevention of Obstructive Nephropathy in Rats

Fattah

Badawi

Mohamed

2019

Egyptian Journal of Histology

View full text Add to dashboard Cite

show abstract

Overlapping of mononuclear cells derived from bone marrow in rats' intervertebral discs: an in vitro study

Abstract: In this study, bone marrow mononuclear cells (BM-MNC) derived from rats were used in order to promote intervertebral disc regeneration. These cells were isolated after centrifugation in a Ficoll

Cited by 1 publication

References 17 publications

Autologous-Versus Allogeneic-Bone Marrow Cell Grafting in Prevention of Obstructive Nephropathy in Rats

Autologous-Versus Allogeneic-Bone Marrow Cell Grafting in Prevention of Obstructive Nephropathy in Rats

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