Técnicas e sítios de coleta de medula óssea em cães e gatos

Müller, Daniel Curvello de Mendonça; Pippi, Ney Luís; Basso, Paula Cristina; Olsson, Débora Cristina; Santos, Evandro dos; Guerra, Antônio Cezar Oliveira

doi:10.1590/s0103-84782009005000153

Cited by 9 publications

(10 citation statements)

References 14 publications

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“…This procedure is suitable when it is desired to obtain cells especially for autologous disease therapy (Muller et al 2009). The aspiration usually occurs in the epiphysis of long bones, such as the greater trochanter of the femur and greater tuberosity of the humeral bone, iliac crest and trans-iliac area in vivo.…”

Section: Bone Marrow Stem Cellsmentioning

confidence: 99%

Bone marrow stem cell applied in the canine veterinary clinics

Gomes

Oliveira²,

Pinheiro

et al. 2017

Pesq. Vet. Bras.

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Cell therapy represents an old therapeutic practice initiated with the transfusion of whole blood in different clinical situations. There is now a breakthrough in the study of multipotent stem cell therapy because of its functionality in regeneration of tissues, which promotes attention of the scientific community. Bone marrow is one of the main sources of multipotent stem cells, composed by hematopoietic stem cells responsible for the renewal of the cellular components of the blood, and mesenchymal stem cells that aid in the regeneration of tissues. These cells have a strong potential for the treatment of several diseases, due their main characteristics such as high plasticity, capacity for self-renewal and immunomodulation. Although, there are many studies that show good results with the use of cell therapy as a form of treatment for several diseases, some studies still show inconclusive or unsatisfactory results. Therefore, the objective of this study was to review the application of bone marrow stem cells in the canine model since improvements on the knowledge of the technique are necessary to enable its applicability with safety and efficacy.

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Section: Bone Marrow Stem Cellsmentioning

confidence: 99%

Bone marrow stem cell applied in the canine veterinary clinics

Gomes

Oliveira²,

Pinheiro

et al. 2017

Pesq. Vet. Bras.

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“…Dessa forma, ao aumentar o número de ossos para colheita, aumentou-se também a quantidade efetiva de MO colhida. Como padronização da técnica, optou-se por colher um quarto do volume total em cada um dos quatro sítios, sendo esses o trocânter maior do fêmur e a cabeça do úmero (Müller et al, 2009) (Strauer e Kornowski, 2003).…”

Section: Resultsunclassified

“…A MO foi colhida utilizando-se a agulha de Steis (11G X 3"), adotando-se, como sítios de colheita, ambos os fêmures e os úmeros, seguindo a técnica proposta por Müller et al (2009). Colheu-se o volume total de MO de 10mL kg -1 , conforme proposto por Olsson et al (2009), contudo optou-se por dividir esse volume aos quatro membros do animal, resultando em 2,5mL kg -1 de MO em cada.…”

Section: Methodsunclassified

Fração total de células mononucleares intra-articular em cães submetidos à reconstrução do ligamento cruzado

Müller

Pippi

Basso³

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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“…When necessary the animals were pre-anesthetized with Acepram® 1% (acepromazine 0.1 mg/kg IM, 30 to 40 minutes before the assay) (MIRÓ et al, 2008;MÜLLER et al, 2009). However, others, still agitated, required general anesthesia, with use of 25 mg/kg of sodic thiopental in order to perform the procedure.…”

Section: Laboratory Tests: Kits Of Canine Visceralmentioning

confidence: 99%

Desempenho do teste rápido imunocromatográfico (TRI) para o diagnóstico da leishmaniose visceral canina: comparação com outros métodos sorológicos em cães suspeitos de Cuiabá, Mato Grosso, Brasil

Santis

Santos

Souza

et al. 2013

Braz. J. Vet. Res. Anim. Sci.

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Performance of DPP™ immunochromathographic rapid test (IRT) for canine visceral leishmaniasis:comparison with other serological methods in suspected dogs from Cuiabá, Mato Grosso State, Brazil AbstractThe immunochromathographic rapid test (IRT) for canine visceral leishmaniasis (CVL) was tested in suspected dogs from the urban area of Cuiabá. The performance of IRT was compared with IFT and EIE and the direct parasitological test (DPT) as the gold standard. The sample, comprising 45 dogs, was selected by the Zoonosis Control Center. Twenty (51%) were male and thirty-four (75.5%) were both mongrel and had an estimated age of less than three years old. According to clinical data and lab tests: 10 (26.3%) dogs have been classified as exposed or infected and 18 (47.4%) as sick. IRT has achieved the best result for sensibility, 62%, high specificity, 87% and for positive and negative predictive values: 83.3% and negative 81.48%, respectively, reinforced by a k coefficient equal to 0.50, representing substantial agreement.Keywords: Canine Visceral Leishmaniasis. Rapid test. Diagnosis. ResumoO teste rápido imunocromatográfico (TRI) para leishmaniose visceral canina (LVC) foi testado em cães suspeitos da área urbana de Cuiabá. O desempenho do teste foi comparado com RIFI e ELISA e, como padrão ouro, o teste parasitológico direto (TPD). A amostra com 45 cães foi selecionada pelo Centro de Controle de Zoonoses, sendo 23 (51,1%) machos, 34 (75,5%) sem raça definida e com idade estimada inferior a três anos. De acordo com os dados clínicos e os exames laboratoriais, 10 (26,3%) cães foram classificados como expostos, outros 10 (26,3%) como infectados e 18 (47,4%) como doentes. O TRI alcançou os melhores resultados quanto à sensibilidade, 62%, elevada especificidade, 87% e para os valores preditivos positivo e negativo: 83,30% e 81,48%, respectivamente, consolidado pelo coeficiente Kappa igual a 0,50, de concordância moderada. Os resultados confirmaram TRI como um bom preditor de doença e infecção para LVC. Palavras-chave:Leishmaniose visceral canina. Teste rápido. Diagnóstico.

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Técnicas e sítios de coleta de medula óssea em cães e gatos

Abstract: Currently

Cited by 9 publications

References 14 publications

Bone marrow stem cell applied in the canine veterinary clinics

Bone marrow stem cell applied in the canine veterinary clinics

Fração total de células mononucleares intra-articular em cães submetidos à reconstrução do ligamento cruzado

Desempenho do teste rápido imunocromatográfico (TRI) para o diagnóstico da leishmaniose visceral canina: comparação com outros métodos sorológicos em cães suspeitos de Cuiabá, Mato Grosso, Brasil

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