Immunogenicity and cross reactivity indices of Streptococcus equi subsp. equi strains isolated from cases of Strangles and commercial vaccines

Moraes, Carina Martins de; Silva, Mariana Sá e; Vargas, Águeda Castagna de; Nogueira, Carlos Eduardo Wayne; Gil-Turnes, Carlos

doi:10.1590/s0103-84782009005000092

Cited by 3 publications

(4 citation statements)

References 12 publications

(9 reference statements)

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“…zooepidemicus. Also, our working hypothesis was based in study conducted by our group in which was demonstrated that mice vaccinated with different S. equi strains isolated from cases of Strangles had serum cross reactivity that range from 0-100%, however, all sera from the vaccinated mice reacts with the rSEM (Moraes et al 2009). Also, following similar approach, we had a very promising results, using the inactivated whole virus BoHV-5 particle associated with a recombinant gD glycoprotein (Araujo et al 2018).…”

Section: Resultsmentioning

confidence: 99%

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Immunogenicity of Streptococcus equi subsp. equi recombinant SeM protein and bacterin in mice

2021

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The infection caused by Streptococcus equi, known as strangles, affects the respiratory system of horses, causing high morbidity and rapid spread among the herd. Bacterin vaccines, composed of inactivated whole cells of S. equi, have variable efficacy and duration. Infected animals produce specific antibodies against SeM, the immunodominant antigen of S. equi. This makes it a promising target for vaccine development. In this context, the objective of this work was to evaluate a vaccine combining S. equi bacterin and recombinant SeM protein. Mice were vaccinated with bacterin (S. equi ~1.2 × 108CFU/ml); rSeM protein (20μg); bacterin-rSeM combination; or PBS (Control Group) and challenged with a suspension of S. equi, containing 10 × LD50. All vaccinated mice survived the challenge and produced anti-rSeM and anti-S. equi antibodies, which were assessed by indirect ELISA. The Control Group reached endpoint criteria 96 h after infection. These results demonstrate that a vaccine combining the S. equi bacterin with rSeM protein protects mice against strangles. This combination vaccine could potentially protect horses and overcome the limitations of currently available strangle vaccines.

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Section: Resultsmentioning

confidence: 99%

“…For bacterin production, a previously characterized isolate of Streptococcus equi subsp. equi was grown in brain heart infusion (BHI, Difco, USA) and shaken at 37°C overnight (Moraes et al 2009). Cultures were centrifuged at 332 × g, and pellets were resuspended in sterile saline buffer.…”

Section: Methodsmentioning

confidence: 99%

Immunogenicity of Streptococcus equi subsp. equi recombinant SeM protein and bacterin in mice

2021

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“…To evaluate the vaccine efficacy vaccinated mice were challenged, on day 42, with a lethal dose of an isolate of S. equi subs. equi, previously characterized [14]. The lethal dose of S. equi was determined by Letal Dose 50 (LD50) assay.…”

Section: Vaccination and Challengementioning

confidence: 99%

Immunogenicity of Escherichia coli Expressing Streptococcus equi subsp. equi Recombinant SeM

Rosa

Conrad

Moraes

et al. 2021

Braz. arch. biol. technol.

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The Equine Strangles, caused by Streptococcus equi subs. equi, is a contagious disease, causing high rates of morbidity been responsible for important economic losses. The M protein synthesized by S. equi plays an important role in the pathogenesis and is a promising candidate for a vaccine antigen. The innate immune system is responsible for the first immune response against microorganisms, this response is mediated by receptors that detect PAMPs and their activation trigger crucial modulation of the adaptative immune response. This work describes the immune response of S. equi subs. equi. recombinant SeM protein, using Escherichia coli BL21 (DE3) as an expression and delivery vaccine system. To characterize and to determine the vaccine efficacy, mice were vaccinated as followed: 1. Recombinant E. coli expressing rSeM protein; 2. The same recombinant E. coli, inactivated adsorbed in Alumen; 3. Purified rSeM protein adsorbed in Alumen; 4. Inactivated S. equi whole cells adsorbed in Alumen; 5. Control group. All vaccinated mice developed protective response against S. equi infection, however the groups that received the E. coli expressing rSeM presented significant higher IgG level than other vaccinated groups.The recombinant E. coli delivery vaccine system also induced a highest IgG response than inactivated S. equi or purified rSeM vaccines in horses. This study evidence that the recombinant E. coli, live or inactivated, enhanced the humoral response, reaching significant higher antibodies levels than those HIGHLIGHTS  E. coli BL21 as a suitable expression and delivery vaccine system;  rSeM protein protect mice against S. equi infection;  E. coli cells enhance the immune response against S. equi;  rSeM protein, purified or not, presented immunogenicity in horses.  Insert a highlight no longer than 85 characters. Insert a highlight no longer than 85 characters.• Insert a highlight no longer than 85 characters. 2 Rosa, M.C.; et al.

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“…From each one, about 6 ml of blood samples was drained from jugular vein ‫التربيــــــة‬ ‫كميــــــــة‬ ‫مجمــــــــة‬ ‫العشرون‬ ‫و‬ ‫الثامن‬ ‫العـــــــــــــــدد‬ by using of a disposable syringe, evacuated in EDTA anticoagulant tubes and centrifuged at 3000 rpm/15 minutes for serum obtaining. The serum samples were saved in numbered 1ml eppendorf safe-lock tubes that kept at -20C until is used (9). Also, the required data related to the region, type (Races or workers), age ( 1 year and  1 year), sex (males and females) and a history of respiratory signs, were reported as epidemiological risk factors.…”

Section: Data and Samples Collectionmentioning

confidence: 99%

Seroepidemiological detection and Culture Utilization for Diagnosis of Carrier Horses and Donkeys with Strangles

Al-Gharban¹

2017

eduj

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This study was aimed for detection of carrier equines with strangles disease through identification of anti-Streptococcus equi antibodies in blood sera of apparently healthy horses and donkeys that inhabitant in different regions belonging to three Iraqi provinces. For this purpose, an overall of 462 equine animals (308 horses and 154 donkeys) were submitted for examining by using of indirect-ELISA that revealed on 22.29% total positive infections, comprised from 29.87% seropositive horses and 7.14% seropositive donkeys. In addition, the culture results of nasopharyngeal swabs samples that obtained, randomly, from 30 horses, seropositively, were revealed on 86.76% positive samples. In horses, the seropositive results in Baghdad, Al-Qadisiyah and Wasit provinces were 28.13%, 30.27% and 30.37%, respectively; while in donkeys; they were 9.41% and 4.35% in Al-Qadisiyah and Wasit provinces, respectively. The seroprevalence in worker and races horses were 30.96% and 18.52% of, respectively, while in age groups, they were 47.54% and 25.51% in of £ 3 years and > 3 years groups, respectively. Also, the seropositive results were 28.68% in males and 30.81% in females; whereas, they were 33.49% of seropositive horses with a history of respiratory signs and 21.88% without it.

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Immunogenicity and cross reactivity indices of Streptococcus equi subsp. equi strains isolated from cases of Strangles and commercial vaccines

Abstract: Immunogenicity and cross reactivity indices of

Cited by 3 publications

References 12 publications

Immunogenicity of Streptococcus equi subsp. equi recombinant SeM protein and bacterin in mice

Immunogenicity of Streptococcus equi subsp. equi recombinant SeM protein and bacterin in mice

Immunogenicity of Escherichia coli Expressing Streptococcus equi subsp. equi Recombinant SeM

Seroepidemiological detection and Culture Utilization for Diagnosis of Carrier Horses and Donkeys with Strangles

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