Técnica de centrífugo-flutuação com sulfato de zinco no diagnóstico de helmintos gastrintestinais de gatos domésticos

Souza-Dantas, Letícia Mattos de; Bastos, Otílio Pereira Machado; Brener, Beatriz; Salomão, M. C.; Guerrero, Jorge; Labarthe, Norma

doi:10.1590/s0103-84782007000300051

Cited by 18 publications

(13 citation statements)

References 5 publications

(4 reference statements)

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“…This sensitivity finding differs from that found in the study of Souza-Dantas et al (2007), who reported a sensitivity of 100% when compared with the necropsy of the intestinal tract of cats.…”

Section: Resultscontrasting

confidence: 99%

Evaluation of the accuracy of parasitological techniques for the diagnosis of intestinal parasites in cats

Rezende

Avelar

Storchilo

et al. 2015

Rev. Bras. Parasitol. Vet.

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The accuracy of the parasitological techniques of Willis, Hoffman-Pons-Janer or Lutz (HPLJ), Sheather and Faust was evaluated in fecal samples from stray cats caught by the Zoonosis Control Center in Goiânia, Goiás, Brazil. These four techniques were applied separately to analyze 154 fecal samples, and their accuracy was analyzed based on an evaluation of their sensitivity, specificity, positive and negative predictive values, and Kappa index, resulting in the selection of the Willis technique as the nominal gold standard. Of the 154 samples, 115 (74.68%) tested positive for intestinal parasites. The analysis of the frequency of positivity indicated that the HPLJ technique detected 86.1% of the positive samples and was the closest to the gold standard. The analysis of the accuracy of the techniques was evaluated using the most prevalent parasites. The Sheather technique showed the highest accuracy in the detection of Ancylostomatidae, while the Sheather and HPLJ techniques showed similar accuracies in the detection of Cystoisospora spp. when compared to the gold standard. Lastly, the Faust technique showed the highest accuracy in the detection of Toxoplasma gondii when compared to the gold standard. This study underscores the importance of combining parasitological techniques in the diagnosis of intestinal parasites in cats.Keywords: Accuracy, intestinal parasites, parasitological techniques, stray cats. ResumoA avaliação da acurácia das técnicas parasitológicas de Willis, Hoffman-Pons-Janer ou Lutz (HPJL), Sheather e Faust foram realizadas em amostras fecais de gatos capturados pelo Centro de Controle de Zoonoses em Goiânia, Goiás. Essas quatro técnicas foram utilizadas separadamente para análise de 154 amostras fecais. A análise de acurácia foi empregada, avaliando-se a sensibilidade, especificidade, valor preditivo positivo, valor preditivo negativo e índice kappa, sendo a técnica de Willis eleita como padrão-ouro. Das amostras analisadas 74,68% (115/154) foram positivas para enteroparasitos. A análise de frequência de positividade demonstrou que a técnica HPJL detectou 86,1% dos positivos, aproximando-se do padrão-ouro. A avaliação da acurácia foi realizada pela análise dos parasitos mais prevalentes, sendo que, para Ancilostomídeos, a técnica de Sheather apresentou melhor acurácia; para Cystoisospora spp., as técnicas de Sheather e HPJL foram semelhantes e para Toxoplasma gondii, a técnica de Faust apresentou melhor acurácia comparada ao padrão ouro. Este estudo demonstra a importância da associação de técnicas parasitológicas para o diagnóstico de enteroparasitos em gatos.Palavras-chave: Acurácia, enteroparasitos, técnicas parasitológicas, gatos errantes.

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Section: Resultscontrasting

confidence: 99%

Evaluation of the accuracy of parasitological techniques for the diagnosis of intestinal parasites in cats

Rezende

Avelar

Storchilo

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…In Poland (Borecka, 2005), Venezuela (Ramírez-Barrios et al, 2004), the United States (Bridger &Whitney, 2009) and other regions of Brazil (Sousa-Dantas et al, 2007), the percentages of Ancylostoma in dogs were lower than that obtained in our studies, which evidenced 64.2% (27/42) positivity for samples collected from the environment. Of this sampling, 10.86% (5/46) were from child day care centers/parks, 41.30% (19/46) from streets/sidewalks and 47.82% (22/46) from squares/gardens.…”

contrasting

confidence: 65%

Endoparasites with Zoonotic Potential in Domesticated Dogs

Bresciani¹,

Coelho²,

Gomes³

et al. 2012

Zoonosis

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“…However, the Faust and Willis technique displayed a better performance (75% sensitivity and Kappa=0.805), and was considered more suitable for the diagnosis of Ancylostomids, because this parasite has light eggs. Souza-Dantas et al (2007) demonstrated the importance of using the Faust technique, which reached 100% sensitivity in comparison to necropsy of the intestinal tract of cats. Coelho et al (2013) also obtained a good performance using Faust compared in other techniques used in its diagnosis of Ancylostomatidae.…”

Section: Resultsmentioning

confidence: 99%

Analysis of the accuracy of different laboratory methods for the diagnosis of intestinal parasites from stray and domiciled cats (Felis catus domesticus) in Goiânia, Goiás, Brazil

Lima

Rezende

Rocha

et al. 2018

Rev. Bras. Parasitol. Vet.

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Cats are carriers of zoonotic agents to humans, including intestinal parasites. The purpose of this study was to analyze the accuracy of different laboratory methods for the diagnosis of intestinal parasites. Fecal samples were processed by the Willis, Sheather, Faust and Hoffman-Janer-Pons-Lutz (HJPL) methods. Accuracy analysis was performed determining the sensitivity, specificity, positive and negative predictive value and Kappa. A total of 149 fecal samples were collected, 65 from stray cats and 84 from domiciled cats. The prevalence of intestinal parasites in stray cats was 60% while in domiciled cats it was 17%. In the analysis of accuracy, the techniques that showed the greatest accuracy for Ancylostomids were Willis and Faust, for Cystoisospora spp. Sheather with Faust or HPJL, and Toxoplasma gondii/Hammondia hammondi the association between Willis and Faust. Therefore, for a reliable evaluation of the prevalence of intestinal parasites, at least two different techniques should be used in parasitological exams of feces.

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Técnica de centrífugo-flutuação com sulfato de zinco no diagnóstico de helmintos gastrintestinais de gatos domésticos

Abstract: (FAUST et al., 1938) ABSTRACT Fecal examinations are the most commonly used laboratory tool for diagnosis of gastrointestinal nematode parasitic infections. Zinc centrifugal floatation technique (FAUST et al., 1938)

Cited by 18 publications

References 5 publications

Evaluation of the accuracy of parasitological techniques for the diagnosis of intestinal parasites in cats

Evaluation of the accuracy of parasitological techniques for the diagnosis of intestinal parasites in cats

Endoparasites with Zoonotic Potential in Domesticated Dogs

Analysis of the accuracy of different laboratory methods for the diagnosis of intestinal parasites from stray and domiciled cats (Felis catus domesticus) in Goiânia, Goiás, Brazil

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