Determinação de rotina do crômio em fezes, como marcador biológico, pelo método espectrofotométrico ajustado da 1,5-difenilcarbazida

Bremer‐Neto, Hermann; Graner, Celso Augusto Fessel; Pezzato, Luiz Edivaldo; Padovani, Carlos Roberto

doi:10.1590/s0103-84782005000300033

Cited by 38 publications

(19 citation statements)

References 10 publications

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“…Chromic oxide (Cr2O3) was used as external inert marker at 0.1% according to Bremer Neto et al (2005). Test ingredients, vitamin and mineral premixes were supplied by Mogiana alimentos S.A.…”

Section: Methodsmentioning

confidence: 99%

“…Samples were analyzed to determine calcium (Ca), phosphorus (P), magnesium (Mg) and copper (Cu) concentrations by flame atomic absorption spectrophotometry on a Shimadzu AA-6800 (Shimadzu, Japan). The chromic oxide content of diets and feces was determined according to Bremer Neto et al, (2005) and the gross energy content was determined in an adiabatic calorimetric bomb (Parr Instrument Company, Moline-IL, USA).…”

Section: Methodsmentioning

confidence: 99%

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Apparent nutrient digestibility and mineral availability of protein-rich ingredients in extruded diets for Nile tilapia

et al. 2012

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-Apparent digestibility coefficients (ADC) of gross energy and dry matter (DM) and mineral availability were evaluated for eight protein-rich ingredients (corn gluten meal, soybean meal, cottonseed meal at 280.0 and 380.0 g/kg, poultry by-product meal, meat meal, fish meal and feather meal) in compound diets for Nile tilapia. .582), and the lowest calcium and phosphorus availability were recorded for cottonseed meal at 280.0 g/kg. Therefore, poultry by-product meal appears to be the best ingredient for using in Nile tilapia diets.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Apparent nutrient digestibility and mineral availability of protein-rich ingredients in extruded diets for Nile tilapia

et al. 2012

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“…Bremer Neto et al (2005) redefined the Graner (1972) method, specifically for fecal chromium evaluation. Those authors did not verify differences between the chromium content estimated by colorimetry using the 1.5-diphenylcarbazide and the chromium content estimated by AAS.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of chromium concentration in cattle feces using different acid digestion and spectrophotometric quantification techniques

Souza

Detmann

Piña

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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The objective of this work was to evaluate combinations between acid digestion techniques and spectrophotometric quantification to measure chromium concentration in cattle feces. Digestion techniques were evaluated based on the use of nitric and perchloric acids, sulfuric and perchloric acids, and phosphoric acid. The chromium quantification in the solutions was performed by colorimetry and by atomic absorption spectrophotometry (AAS). When AAS was used, the addition of calcium chloride to the solutions as a releasing agent was also evaluated. Several standard samples containing known chromium contents were produced (0, 2, 4, 6, 8 and 10g of chromium per kg of feces) using cattle feces obtained from three different animals to evaluate the accuracy of the different combinations of techniques. The accuracy was evaluated by adjusting a simple linear regression model of the estimated values on the actual values of chromium content in the standard samples. Regardless of the digestion technique, the chromium content estimates in the standard samples obtained by colorimetry were not accurate (P<0.05). Considering the AAS quantification, the digestion techniques based on nitric and perchloric acids and based on sulfuric and perchloric acids provided complete chromium recovery (P>0.05). The use of the digestion technique in phosphoric acid provided incomplete recovery of the fecal chromium (P<0.05). Subsequently, the digestion techniques in nitric and perchloric acids and digestion in sulfuric and perchloric acids, both evaluated by AAS, were compared using 84 cattle feces samples. The results indicate that these techniques provide similar (P>0.05) fecal chromium contents.Keywords: atomic absorption, colorimetry, external markers, chromic oxide (0, 2, 4, 6, 8 RESUMO Objetivou-se avaliar combinações entre técnicas de digestão ácida e quantificação espectrofotométrica para estimar a concentração de cromo em amostras de fezes bovinas. Foram avaliadas técnicas de digestão baseadas na utilização de ácidos nítrico e perclórico, ácidos sulfúrico e perclórico e ácido fosfórico. A quantificação da concentração de cromo nas soluções foi realizada por colorimetria e por espectrofotometria de absorção atômica (EAA). Na quantificação por EAA, foi avaliada a adição de cloreto de cálcio como agente de liberação. Amostras-padrão contendo quantidades conhecidas de cromo foram produzidas

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“…Total phosphorus concentration was analyzed by a colorimetric process, using the vanado-molybdate reagent (Williams, 1979), and amino acid content was determined by high-performance liquid chromategraphy (HPLC) in an auto-analyzer after hydrolysis of samples with 6 N HCl for 22 h at 110ºC (Moore & Stein, 1963). The Cr2O3 content of diets and feces were determined according to Bremer-Neto et al (2005).…”

Section: Digestibility Assaymentioning

confidence: 99%

Replacement of soybean meal by peanut meal in diets for juvenile Nile tilapia, Oreochromis niloticus

Silva

Damasceno

Rocha

et al. 2017

lajar

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ABSTRACT. The use of alternative feeds aims to maintain productivity and reduce animal production costs. This objective of this study was to determine the nutritional value of peanut meal (PNM), as well as replacement of soybean meal (SBM) by PNM in diets for Nile tilapia, Oreochromis niloticus. To determine the apparent digestibility coefficients (ADC) of nutrients from the PNM, 40 fish (100 ± 4.3 g) were randomly distributed in four 250 L tanks and fed reference and test diets (50% PNM) plus 0.1% chromic oxide. Feces were collected by modified Guelph system. For the growth performance trial, 180 fish (13.4 ± 0.2 g) were randomly distributed in 30 200 L tanks and fed during 90 days with isoproteic (26.8% digestible protein) and isoenergetic (17.6 kJ g -1 digestible energy) diets containing replacement levels of 0, 25, 50, 75 and 100% of SBM digestible protein by PNM digestible protein. The experimental design was completely randomized with five treatments and six replicates. The ADC for protein from PNM was 90.9% whereas the ADCs for essential amino acids ranged from 88.7% for lysine to 97.6% for arginine. The feed conversion ratio was significantly affected when the SBM was totally replaced by PNM. The protein efficiency ratio, protein retention and whole-body protein content significantly decreased in fish fed diets containing PNM levels above 25% of PNM. Therefore, PNM can replace up to 25% of SBM without impairing juvenile Nile tilapia growth performance, feed efficiency, and body composition.

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Determinação de rotina do crômio em fezes, como marcador biológico, pelo método espectrofotométrico ajustado da 1,5-difenilcarbazida

Cited by 38 publications

References 10 publications

Apparent nutrient digestibility and mineral availability of protein-rich ingredients in extruded diets for Nile tilapia

Apparent nutrient digestibility and mineral availability of protein-rich ingredients in extruded diets for Nile tilapia

Evaluation of chromium concentration in cattle feces using different acid digestion and spectrophotometric quantification techniques

Replacement of soybean meal by peanut meal in diets for juvenile Nile tilapia, Oreochromis niloticus

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