Viabilidade de sêmen suíno armazenado a 5°C de acordo com a taxa de resfriamento e incubação prévia

Katzer, Lia H.; Bernardi, Mari Lourdes; Bortolozzo, Fernando Pandolfo; Wentz, Ivo

doi:10.1590/s0103-84782005000100022

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…Desta maneira o fator mais importante durante o processo de refrigeração ainda parece ser a sensibilidade dos espermatozoides a temperatura de preservação, sendo os melhores resultados de motilidade observados entre todas as amostras refrigeradas a 15 °C. Katzer et al (2005) avaliaram a viabilidade do sêmen suíno refrigerado a temperaturas de 5 e 17 °C em meio BTS ("Beltsville Thawing Solution") e observaram motilidade superior quando o sêmen foi preservado a 17 °C. Da mesma forma, Althouse et al (1998) superior a 75% nas amostras preservadas entre 12 e 17 °C, durante 48 horas de refrigeração, e motilidade inferior a 70% nas amostras mantidas entre 8 e 10 °C, sugerindo que a temperatura crítica para resfriamento do sêmen suíno encontra-se a abaixo de 12 °C.…”

Section: Discussionunclassified

Efeito da Vitamina E (±-Tocoferol) e de Diferentes Temperaturas na Viabilidade de Sêmen Suíno Refrigerado.

Labêta¹,

Mello²,

Silenciato³

et al. 2017

Braz. J. Vet. Med.

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Copyright Labêta et al. Este é um artigo publicado em acesso aberto (Open Access) sob a licença Creative Commons Attribution Non-Commercial, que permite uso, distribuição e reprodução em qualquer meio, sem restrições desde que sem fins comerciais e que o trabalho original seja corretamente citado. ) com nível de significância de 5%. Houve diferença significativa (p<0,05) para a motilidade, quando foram comparadas as temperaturas de 5 e 15 °C independentemente do uso do α-Tocoferol durante o período entre 12 e 72 horas. Os valores mais altos de motilidade foram obtidos com a temperatura de 15 °C. Observou-se também diferença (p<0,05) para a motilidade entre temperaturas de 5 e 15 °C quando comparado apenas com as amostras acrescidas de α-Tocoferol, bem como as amostras sem esta substância. A maior motilidade foi obtida novamente com a temperatura de 15 °C. Não foi observada diferença no vigor e na integridade da membrana (teste hiposmótico) entre as amostras adicionadas ou não de α-Tocoferol, em qualquer uma das temperaturas e tempos estudados. Conclui-se que a temperatura de 15 °C foi mais eficiente para a conservação de sêmen de cachaços durante 72 horas. Conclui-se também que a adição de 200 μg/mL de α-Tocoferol não melhorou a viabilidade dos espermatozoides submetidos à refrigeração. Efeito da vitamina E (α-TocoferolPalavras-chave: ejaculado, refrigeração, cachaço. AbstractThe objective of this study was to evaluate the addition of α-Tocopherol to swine insemination doses stored at 5 and 15 °C for a period of 72 hours. Semen samples (n=9) collected from three adult Pietrain boars were treated with or without 200 μg/mL of α-Tocopherol. The treatments were: T1: α-Tocopherol + cooling to 5 °C; T2: α-Tocopherol + cooling to 15 °C; T3: no α-Tocopherol + cooling to 5 °C; T4: no α-Tocopherol + cooling to 15 °C. All samples were evaluated up to 72 hours for sperm motility and intensity of movements every 12 hours, and the hiposmotic test was performed every 24 hours. The average of the motility, the intensity of movements and hiposmotic test (HOST) were analyzed by chi-square test (х 2 ) with 5% significance level. There was a significant difference (p <0.05) for motility when comparing the temperatures of 5 and 15 °C regardless of α-Tocopherol during the period between 12 and 72 hours. The highest motility values were obtained at 15 °C. It was also observed a difference (p <0.05) for sperm motility between temperatures of 5 to 15 °C when compared only the samples with α-Tocopherol, as well as the samples without this substance. The higher motility was obtained with 15 °C. No difference was observed for the intensity of movements and membrane integrity between samples added or not of α-Tocopherol, in any of the temperatures and times evaluated. We concluded that the temperature of 15 °C was more efficient for boar semen preservation over 72 hours. It is also concluded that the addition of 200 μg/mL of α-Tocopherol showed no improvement in the viability of cooled porcine semen.

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Section: Discussionunclassified

Efeito da Vitamina E (±-Tocoferol) e de Diferentes Temperaturas na Viabilidade de Sêmen Suíno Refrigerado.

Labêta¹,

Mello²,

Silenciato³

et al. 2017

Braz. J. Vet. Med.

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Antibiotic-free extended boar semen preserved under low temperature maintains acceptable in-vitro sperm quality and reduces bacterial load

et al. 2020

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Fontes de óleo e níveis de suplementação de vitamina E na ração sobre a qualidade do sêmen suíno acondicionado a 17 e 5ºC

et al. 2010

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Oil sources and levels of vitamin E supplementation in the diet on the quality of boar semen cooled at 17 and 5°CABSTRACT -The objective of this study was to evaluate the effect of the addition of oil sources and levels of vitamin E supplementation in the rations on the characteristics of boar semen cooled at 17 and 5ºC. It was used twenty-four Dalboar 85 boars distributed in a complete random design, in a 2 × 3 factorial scheme, with two sources of oil (soybean and salmon) and three levels of antioxidant (150, 300 and 450 vitamin E mg/kg). For animals under supplementation with salmon oil, the motility and hypoosmotic swelling test (HOST) of sperm after 24, 48, and 72 hours was higher than the ones observed in animals fed soybean oil ration. Salmon oil increased the vigor in both temperatures evaluated after 24 and 48 hours. The occurence of total morphological abnormalities was higher in the semen of animals fed soybean oil and cooled at 17ºC while in the lowest cooling temperature (5ºC) there was no difference among animals under supplementation. The abnormal morphology in the semen at 5ºC was higher than in the semen cooled at 17ºC. Salmon oil improves the sperm characteristics of the boar semen cooled at 17 and 5ºC from 24 to 48 hours.

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Viabilidade de sêmen suíno armazenado a 5°C de acordo com a taxa de resfriamento e incubação prévia

Cited by 3 publications

References 15 publications

Efeito da Vitamina E (±-Tocoferol) e de Diferentes Temperaturas na Viabilidade de Sêmen Suíno Refrigerado.

Efeito da Vitamina E (±-Tocoferol) e de Diferentes Temperaturas na Viabilidade de Sêmen Suíno Refrigerado.

Antibiotic-free extended boar semen preserved under low temperature maintains acceptable in-vitro sperm quality and reduces bacterial load

Fontes de óleo e níveis de suplementação de vitamina E na ração sobre a qualidade do sêmen suíno acondicionado a 17 e 5ºC

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