Acute toxicity of nitrite to matrinxã, Brycon cephalus (Günther, 1869), (Teleostei-Characidae)

Avilez, Ive Marchioni; Aguiar, Lúcia Helena de; Altran, Alexandre Eneas; Moraes, Gilberto

doi:10.1590/s0103-84782004000600013

Cited by 13 publications

(4 citation statements)

References 24 publications

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“…This control should be even more stringent when determining the tolerance of a given species to a stressor agent, in order to assure reliable results, which dictate the animal tolerance limits (Lima 2003;Avilez et al 2004;Cavero et al 2004). For example, studies involving Piaractus mesopotamicus indicate that water temperature seems to have an influence on the mortality rates caused by A. hydrophila and septicemia outbreaks are associated with high water temperatures (Garcia et al 2009).…”

Section: Discussionmentioning

confidence: 99%

LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus

et al. 2011

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In order to determine the lethal dose (96-h LD 50 ) of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus, to be applied in challenge tests, 90 fish (63.23 ± 6.39 g) were divided into five treatments, with different bacterial solutions: T 1 -Control (0.9% NaCl saline solution); T 2 (4 x 10 11 cells/ mL); T 3 (5 x 10 11 cells/ mL); T 4 (1.36 x 10 12 cells/ mL) and T 5 (3.06 x 10 12 cells/ mL). Fish were previously anesthetized with benzocaine (60 mg L -1 ), inoculated in the peritoneal cavity with the bacterial suspensions and then distributed into fifteen 80-L test chambers, where the water variables were monitored and fish mortality was observed. The experiment was randomly designed in three replicates and the 96-h LD 50 was estimated according to the trimmed Spearman-Karber method. Water quality variables remained within adequate ranges for fish health and performance. Fish mortality rate increased with the bacterial concentrations of A. hydrophila (T 1 = 0%; T 2 = 16.66%; T 3 = 44.44%; T 4 = 72.22% and T 5 = 100%), and the first mortalities were observed after 57 h, although the signs of the bacterial infection were already observed 24 h after the inoculation. The results indicate that the 96-h LD 50 value of A. hydrophila to matrinxã is 6.66 x 10 11 cells/ mL. Keywords: Aeromonas hydrophila, 96-h LD 50 , matrinxã, tropical fishes DL 5o da bactéria Aeromonas hydrophila para o matrinxã, Brycon amazonicus resumo Para determinar a dose letal (DL 50 96-h) da bactéria Aeromonas hydrophila para o matrinxã, Brycon amazonicus, com aplicabilidade para testes de desafio, foram utilizados 90 peixes (63,23 ± 6,39 g), divididos em cinco tratamentos, com diferentes soluções bacterianas: T 1 -Controle (solução salina 0,9% NaCl); T 2 (4 x 10 11 células/ mL); T 3 (5 x 10 11 células/ mL -1 ); T 4 (1,36 x 10 12 células/mL -1 ) e T 5 (3,06 x 10 12 células/ mL -1 ). Os peixes foram previamente anestesiados com benzocaína (60 mg L -1 ), inoculados na cavidade peritoneal com as suspensões bacterianas e distribuídos em 15 aquários de vidro de 80 L de capacidade, com aeração constante. O experimento teve duração de 96 h, no qual foram monitoradas a mortalidade e a qualidade da água. O delineamento experimental foi inteiramente casualisado com três réplicas e a DL 50 96-h foi estimada de acordo com o método Spearman-Karber. Durante o experimento os parâmetros físico-químicos da água permaneceram dentro das condições consideradas adequadas para o desenvolvimento e saúde dos organismos aquáticos. A mortalidade dos peixes aumentou nas concentrações crescentes de A. hydrophila (T 1 = 0%; T 2 = 16,66%; T 3 = 44,44%; T 4 = 72,22% e T 5 = 100%), contudo, as primeiras mortalidades ocorreram em 57 h após a inoculação das concentrações bacterianas, sendo observados os primeiros sinais de infecção em 24 h após a inoculação. Os resultados indicam que o valor da DL 50 96-h da bactéria A. hydrophila para o matrinxã foi igual a 6,66 x 10 11 células/mL de solução salina. PAlAvrAs-chAve: Aeromonas hydrophila, DL 50 96-h, matrinxã...

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Section: Discussionmentioning

confidence: 99%

LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus

et al. 2011

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“…After the acclimation period silver catfish (7.16 ± 0.27 g) were transferred to 2 L aquaria (10 fish each, 3 replicates) and were exposed for 96 h to the following waterborne NO 2 -levels (in mg.L -1 ): 0.08 ± 0.07, 5.33 ± 0.07, 10.66 ± 0.05, 21.33 ± 0.09, and 42.66 ± 0.04. These levels were maintained through the addition of sodium nitrite (NaNO 2 , Merk, 99.5% purity) and were chosen based on previous LC 50-96h experiments with neotropical species (Bianchini et al, 1996;Avilez et al, 2004;Costa et al, 2004). All feces and residues were removed daily by suction, and consequently approximately 40% of the water in the boxes was replaced by water with previously adjusted nitrite levels.…”

Section: Methodsmentioning

confidence: 99%

“…An increase of waterborne NO 2 -levels induces NO 2 -accumulation in fish blood and tissues from 10 to 100 times the environmental concentration (Holt & Arnold, 1996;Costa et al, 2004) , which produces toxic derivatives with deleterious effects on physiological processes (Jensen et al, 1987). Despite the importance of such effects, the lethal concentration at 96 h (LC 50-96h ) for NO 2 -was determined only in a few neotropical freshwater fish species (Avilez et al, 2004;Costa et al, 2004), and the effect of a single level of this nitrogenous compound for 8 h on biochemical parameters was analyzed in traira, Hoplias malabaricus (Moraes et al, 1998), pacu, Piaractus mesopotamicus and the hybrid tambacu (Piaractus mesopotamicus x Colossoma macropomum) .…”

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confidence: 99%

Survival, growth and metabolic parameters of silver catfish, Rhamdia quelen, juveniles exposed to different waterborne nitrite levels

et al. 2011

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High nitrite (NO 2 -) levels may develop in aquaculture systems due to high fish density, but studies of lethal concentration values and the effect of NO 2 -on metabolic parameters and growth are scarce. Consequently, in this study was verified the lethal concentration at 96 h (LC 50-96h ) for (NO . In addition, glucose levels in muscle and liver tissues were significantly lower in silver catfish exposed to the highest NO

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“…Matrinxã (Brycon amazonicus), Labeo rohita, walleye (Sander vitreus) and rainbow trout (Oncorhynchus mykiss) exposed to high NO 2 -levels presented lower hematocrit, total hemoglobin, and number of red blood cells than control fish (AVILEZ et al, 2004;MADISON and WANG, 2006;KROUPOVA et al, 2008;CIJI et al, 2013). Conversely, exposure to 1.3 mg L -1 NO 2 -did not affect hematological parameters in silver catfish.…”

Section: Coltmentioning

confidence: 93%

Interação do cálcio e nitrito na água: sobrevivência, crescimento, parí¢metros hematológicos e metabólicos em jundiá

Neves¹,

Souza²,

Santos³

et al. 2017

Bol. Inst. Pesca

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The objective of this study was to determine the effect of different waterborne nitrite (NO 2 -) and calcium (Ca 2+ ) levels on growth, biochemical and hematological parameters of silver catfish juvenile (Rhamdia quelen). Fish were submitted to low (0.05 mg L -1 ) or high (1.3 mg L -1 ) NO 2 and low (7 mg L -1 ) or high (14 mg L -1 ) Ca 2+ levels (four replicates) for 60 days. At the end of the experimental period, fish exposed to high NO 2 showed lower weight gain, biomass and specific growth rate than those maintained at low NO 2 , irrespective of Ca 2+ levels.

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Acute toxicity of nitrite to matrinxã, Brycon cephalus (Günther, 1869), (Teleostei-Characidae)

Abstract: ABSTRACT

Cited by 13 publications

References 24 publications

LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus

LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus

Survival, growth and metabolic parameters of silver catfish, Rhamdia quelen, juveniles exposed to different waterborne nitrite levels

Interação do cálcio e nitrito na água: sobrevivência, crescimento, parí¢metros hematológicos e metabólicos em jundiá

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Acute toxicity of nitrite to matrinxã, Brycon cephalus (Günther, 1869), (Teleostei-Characidae)

Abstract: ABSTRACT

Cited by 13 publications

References 24 publications

LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus

LD5o of the bacteria Aeromonas hydrophila to matrinxã, Brycon amazonicus

Survival, growth and metabolic parameters of silver catfish, Rhamdia quelen, juveniles exposed to different waterborne nitrite levels

Interação do cálcio e nitrito na água: sobrevivência, crescimento, parí­¢metros hematológicos e metabólicos em jundiá

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Interação do cálcio e nitrito na água: sobrevivência, crescimento, parí¢metros hematológicos e metabólicos em jundiá