Aspectos fenotípicos, genotípicos e de diagnóstico da bactéria A. pleuropneumoniae

Costa, Mateus Matiuzzi da; Klein, Cátia Silene; Collares, Roberta Mattos; Vaz, C. S. L.; Balestrim, Raquel; Schrank, Augusto; Silva, Sergio Ceroni da; Piffer, Itamar Antônio; Shrank, Irene Silveira

doi:10.1590/s0103-84782004000400057

Cited by 1 publication

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References 38 publications

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“…Epidemiologic studies must aim to analyze the distribution of a sample within an area (Costa et al, 2004). Although not many groups reflected these two geographic regions, the isolates from Malaysia and from the pulmonary secretion demonstrated the low similarity among the strains.…”

Section: Discussionmentioning

confidence: 99%

Genetic variability in isolates of Chromobacterium violaceum from pulmonary secretion, water, and soil

et al. 2016

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ABSTRACT. Chromobacterium violaceum is a free-living Gramnegative bacillus usually found in the water and soil in tropical regions, which causes infections in humans. Chromobacteriosis is characterized by rapid dissemination and high mortality. The aim of this study was to detect the genetic variability among C. violaceum type strain ATCC 12472, and seven isolates from the environment and one from a pulmonary secretion from a chromobacteriosis patient from Ilhéus, Bahia. The molecular characterization of all samples was performed by polymerase chain reaction (PCR) sequencing and 16S rDNA analysis. Primers specific for two ATCC 12472 pathogenicity genes, hilA and yscD, as well as random amplified polymorphic DNA (RAPD), were used for PCR amplification and comparative sequencing of the products. For a more specific approach, the PCR products of 16S rDNA were digested with restriction enzymes. Seven of the samples, including type-strain ATCC 12472, were amplified by the hilA primers; these were subsequently sequenced. Gene yscD was amplified only in type-strain ATCC 12472. MspI and AluI digestion revealed 16S rDNA polymorphisms. This data allowed the generation of a dendogram for

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Section: Discussionmentioning

confidence: 99%