“…DNA purity (with regard to the presence of enzyme inhibitors) was tested by PCR amplification of fragments of the plastid gene, trnL-trnF. Samples of DNA from mate leaves has successfully been extracted using kits (Cascales et al, 2014;Gottlieb and Poggio, 2014) and protocols using CTAB buffer (Vidor et al, 2002a;Vidor et al, 2002b;Wendt et al, 2007;Friedrich et al, 2017). In both techniques, fresh samples (Friedrich et al, 2017), samples dried with silica gel (Cascales et al, 2014;Gottlieb and Poggio, 2014) or frozen samples kept at -80ºC can used (Wendt et al, 2007;Kubiak et al, 2010).…”