Bilateral lamellar keratoplasty in descemetocele treatment in dog with botulism by use of equine renal capsule and conjunctival pedicle graft

To possibly reduce postoperative adhesions that occur after ocular myoplasties, we investigated the topical effects of 0.04% mitomycin C on the repaired areas of the medial rectus muscle using an equine renal capsule preserved in 98% glycerin for reinforcement of the sutures. Twenty-four rabbits, divided into two groups of 12 animals each [untreated (control) and treated group (MMC)], were submitted to surgical rupture of the medial rectus muscle of one eye and repair of the defect 24 h later with sutures and an equine renal capsule. Post-operative prophylactic treatment of the two groups consisted of the administration of eye drops containing neomycin, polymyxin B and dexamethasone at regular 6-h intervals for eight consecutive days and daily rinsing with physiological saline. MMC animals received additional treatment with topical 0.04% mitomycin C every 6 h for 14 consecutive days. Slit lamp biomicroscopy showed greater irritation of the ocular surface in MMC animals during the first days post operatively. Adhesions were observed at 15 and 30 days of assessment in the two groups, but were more extensive in control animals at 60 days. Histopathology revealed inflammatory exudation in both groups, which was greater in MMC animals. Mitomycin C (0.04%) instilled at 6-h intervals for 14 consecutive days reduced the occurrence of fibrosis in the myoplastic areas. However, the equine renal capsule was found to be of little benefit for the reinforcement of myoplasties.

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“…evaluated equine renal capsules for the reconstitution of dog sclera, and Andrade et al 10,11 . and Laus et al 12 . used them for corneal repair.…”

Section: Introductionmentioning

confidence: 99%

Use of topical mitomycin C in myoplasty of the medial rectus muscle of rabbits

Mamede

Laus

Cabral

et al. 2004

Veterinary Ophthalmology

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Use of fresh autogenous vaginal tunic in the experimental lamellar keratoplasty in dogs (Canis familiaris, LINNAEUS, 1758)

Galera

Laus

Ferreira

2000

Braz. J. Vet. Res. Anim. Sci.

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Use of an allogenous tunica vaginalis conserved in 98% glycerine for keratoplasty in dogs

Vicenti¹,

Laus²,

Neto³

et al. 2002

RBCV

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We evaluated the use of an allogenous tunica vaginalis conserved in 98% glycerine for lamellar keratoplasty in dogs. Seven dogs were submitted to keratoplasty and clinically evaluated during the initial (1 to 15 days), intermediate (16 to 30 days) and late (31 to 120 days) postoperative periods. The corneas were evaluated by light microscopy at 1, 3, 7, 15, 30, 60 and 120 days after surgery. Photophobia, epiphora, blepharospasm, corneal edema, ocular secretion and chemosis were observed during the initial periods, and tended to disappear during the intermediate periods. Vascular neoformation started around the 4th day, with a maximum intensity on the 25th day and a gradual regression thereafter, and tended to disappear between the 60th and 120th day. The graft itself and adjacent areas showed a tendency towards the reestablishment of transparency during the late postoperative periods. With respect to morphology, an inflammatory infiltrate characterized by the invasion of polymorphonuclear and mononuclear leukocytes was observed which disappeared by the 60th postoperative day, when the graft was found to be incorporated and the stroma returned to normal. The present results led us to conclude that the allogenous tunica vaginalis conserved in 98% glycerine was feasible and efficient for lamellar keratoplasty in dogs.Keywords: cornea, keratoplasty, tunica vaginalis, allogenous graft, dogs. ResumoAvaliou-se a túnica vaginal alógena, conservada em glicerina a 98%, na ceratoplastia lamelar em cães. Sete cães foram submetidos à ceratoplastia e avaliados clinicamente em períodos iniciais (1 a 15 dias), intermediários (16 a 30 dias) e tardios (31 a 120 dias) de pós-operatório. As córneas foram avaliadas em microscopia de luz aos um, três, sete, 15, 30, 60 e 120 dias de pós-operatório. Fotofobia, epífora, blefarospasmo, edema corneano, secreção ocular e quemose foram observados nos períodos iniciais, tendendo a desaparecerem nos períodos intermediários. Neoformação vascular iniciou-se próximo aos dos quatro dias, apresentando intensidade máxima aos 25 dias de pós-operatório, quando iniciou regressão gradativa para tender à ausência entre 60 e 120 dias. Nas áreas próprias e adjacentes aos enxertos houve tendência ao reestabelecimento da transparência nos períodos tardios de pós-operatório. Quanto à morfologia, o infiltrado inflamatório caracterizou-se pela invasão de leucócitos polimorfonucleares e mononucleares, tendendo à ausência aos 60 dias de pós-operatório, quando notou-se que o enxerto foi incorporado e o estroma tendeu à normalidade. Os resultados obtidos com a pesquisa permitem admitir que a túnica vaginal alógena, conservada em glicerina a 98%, mostrou-se factível e eficaz na ceratoplastia lamelar em cães.Palavras-chave: córnea, ceratoplastia, túnica vaginal, enxerto alógeno, cães. lntroductionAmong the lesions affecting the cornea, ulcers, abrasions, lacerations and perforations characterized by the loss of epithelium and of variable portions of the stroma have been emphasized (Startup, 1984). Particula...

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Bilateral lamellar keratoplasty in descemetocele treatment in dog with botulism by use of equine renal capsule and conjunctival pedicle graft

Cited by 3 publications

References 9 publications

Use of topical mitomycin C in myoplasty of the medial rectus muscle of rabbits

Use of topical mitomycin C in myoplasty of the medial rectus muscle of rabbits

Use of fresh autogenous vaginal tunic in the experimental lamellar keratoplasty in dogs (Canis familiaris, LINNAEUS, 1758)

Use of an allogenous tunica vaginalis conserved in 98% glycerine for keratoplasty in dogs

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