“…Shoot buds formed were isolated and transferred to a same composition culture medium without plant growth regulator for elongation. It has been observed for various legumes that high concentration of cytokinin stimulated callus and subsequently shoot bud formation (Malik & Saxena, 1992;Dorneles et al, 1994), increased their survival (Dorneles et al, 1994) but also inhibited shoot growth (Thomé et al 1995). Other studies with legumes in vitro (Dewan et al, 1992;Mohamed et al, 1992;Murthy et al, 1996) have determined the need of cytokinin for shoot differentiation and bud multiplication.…”