Avaliação Clínica Preliminar Do Fechamento Comparativo De Incisões Corneanas Com Sutura E Biofill (Película Celulósica)

Schoenau, Luciana Silveira Flores; Pippi, Ney Luís; Schossler, João Eduardo Valau

doi:10.1590/s0103-84781993000200010

Cited by 8 publications

(10 citation statements)

References 4 publications

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“…One of them could be the fact that the implants move together with the orbital content, and the external prostheses could cause repeated traumas on the conjunctival surface; therefore, there construction of the anophthalmic cavity with an integrated polymer with a soft and smooth surface could help decrease the frequency of orbital implant extrusion (4,(10)(11)(12) . In the medical pharmaceutical industry, cellulose is being tested as a replacement for blood vessels, as an envelope for peripheral nerves, a framework of cartilaginous tissues, a substratum for cell culture, a biological dressing for burns, and for use in the fabrication of numerous prostheses, including dressings in veterinary ophthalmology (13)(14)(15)(16)(17)(18)(19) . A polymer of cellulose, obtained by bacterial synthesis from sugar cane molasses, has been studied in the Industrial Microbiology La boratory at Carpina Experimental Station of Sugarcane / Rural Univer sity of Pernambuco, since 1990.…”

Section: Introductionmentioning

confidence: 99%

Use of a gel biopolymer for the treatment of eviscerated eyes: experimental model in rabbits

Cordeiro-Barbosa¹,

Aguiar²,

Lira³

et al. 2012

Arq. Bras. Oftalmol.

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Purpose: To evaluate histologically the integration process of cellulose gel produced by Zoogloea sp when implanted into rabbits' eviscerated eyes. Methods: This experimental study employed 36 eyes of 18 rabbits subjected to evis ceration of their right eyes. The sclerocorneal bag was sutured and filled with biopolymer from sugar cane in the gel state. All animals were clinically examined by biomicroscopy until the day of their sacrifice which occurred on the 7 th , 30 th , 60 th , 90 th , 120 th , or 240 th day. The eyeballs obtained, including the left eyes considered controls were sent for histopathological study by optical macroscopy and microscopy. Tissue staining techniques used included hematoxylin-eosin, Masson trichrome (with aniline), Gomori trichrome, Van Gienson, Picrosirius red, and periodic acid-Schiff (PAS). Results:No clinical signs of infection, allergy, toxicity, or extrusion were observed throughout the experiment. The corneas were relatively preserved. Macroscopic examination revealed a decrease of ~ 8% in the volume of the bulbs implanted with the biopolymer. After cutting, the sclerocorneal bag was solid, compact, elastic, and resistant to traction, with a smooth and whitish surface, and showed no signs of necrosis or liquefaction. The episcleral tissues were somewhat hypertrophied. The histological preparations studied in different colors revealed an initial lymphoplasmacytic infiltration, replaced by a fibroblastic response and proliferation of histiocytes, along with formation of giant cells. Few polymorphonuclearneutrophils and eosinophils were also found. Neovascularization and collagen deposition were present in all animals starting from day 30; although on the 240 th day of the experiment the chronic inflammatory response, neovascularization and collagen deposition had not yet reached the center of the implant. Conclusion: In this model, the cellulose gel produced by Zoogloea sp proved to be biocompatible and integrated into the orbits. Morphometric, immunohistochemical and biodegradability studies should be performed in the future. Keywords

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Section: Introductionmentioning

confidence: 99%

Use of a gel biopolymer for the treatment of eviscerated eyes: experimental model in rabbits

Cordeiro-Barbosa¹,

Aguiar²,

Lira³

et al. 2012

Arq. Bras. Oftalmol.

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“…Prompt and effective repair of deep or large‐diameter corneal defects is desirable in order to maintain or restore vision. Several methods of surgical management of canine corneal ulcers, including microsurgery, corneal and conjunctival grafting, and use of synthetic suture materials, have been proposed and developed in the last 20 years such that the prognosis for large, ulcerative lesions has improved 1–18 …”

Section: Introductionmentioning

confidence: 99%

“…The treatment of ulcerative corneal lesions can be attempted in several ways: temporary tarsorrhaphy, 1 third eyelid flap, 2 transposition of third eyelid tissue, 3 conjunctival patch graft, 4 conjunctival pedicle graft, 5 tissue adhesive, 6 , 7 contact lens, 8 corneal‐scleral transposition, 9 autogenous corneal graft, 10 , 11 , 12 homologous corneal graft, 13 , 14 , 15 and synthetic materials 16 , 17 , 18 …”

Section: Introductionmentioning

confidence: 99%

The use of xenologous amniotic membrane to repair canine corneal perforation created by penetrating keratectomy

Barros

Garcia

Laus

et al. 1998

Veterinary Ophthalmology

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This study was performed to evaluate the use of glycerol-preserved equine amniotic membrane as replacement for full-thickness corneal defects in dogs. Eighteen mixed-breed dogs were used. A perilimbal, full-thickness, 5 mm square corneal defect was created surgically, and a donor implant of equine amniotic membrane of the same size and shape sutured in place with 10-0 nylon simple interrupted sutures. Corneal edema was observed near the implant 24 h after surgery, but was absent after 1 week. Granulation tissue and corneal vascularization superficial to the implant were noticed on postoperative day 7, but were absent on day 30. Corneal vascularization persisted until the end of the experiment. There was no fluorescein retention by postoperative day 30. There was slight clearing of the corneal implant by postoperative 30, and slight pigmentation of the donor implant observed at postoperative day 180. An acute inflammatory process as well as fibroblasts were present at early postoperative stages. At postoperative day 60 there was no inflammatory cellular infiltrate, but fibroblasts and fibrosis were present. Corneal architecture was restored at the end of the experiment, with a layering of the epithelium-stroma-debris of amniotic membrane-stroma-endothelium present, and pigmentation and vascularization present in the deep layers of the cornea. Although vascularization indicated some degree of graft rejection, the clinical and histological evidence indicates that the xenologous amniotic membrane can be useful as a tectonic graft in the repair of full-thickness lesions of the cornea of dogs.

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“…However, new reparative materials had been tested in the field of veterinary ophthalmology; and include xenologous pericardium, homologous peritoneum, xenologous renal capsule, sardine scale, xenologous and homologous amniotic membrane, homologous vaginal membrane, porcine small intestine submucosa (BUSSIERES et al, 2004;SAMPAIO et al, 2006;GALERA et al, 2009). Within this context, microfibrillar cellulose membranes have been used for the temporary replacement of skin in burns, abrasions, ulcers, or cases of tissue loss (REBELLO et al 1987;PIPPI & SAMPAIO, 1990;DALECK & BECHARA 1991;SCHOENAU et al, 1993). These membranes consist of polysaccharides formed by thousands of molecules of cellobiose synthesized by the bacterium Acetobacter xylinum.…”

Section: Introductionmentioning

confidence: 99%

“…PIPPI & SAMPAIO (1990) used this membrane in an experimental study on the repair of lamellar corneal lesions in rabbits. SCHOENAU et al (1993) successfully repaired corneal defects using suture and a cellulose membrane; the authors reported faster regeneration of the corneal epithelium and reduced infiltration by polymorphonuclear cells. However, it is known that no recent studies have been carried out regarding the impact of such material in corneal cell proliferation by immunohistochemistry.…”

Section: Introductionmentioning

confidence: 99%

Experimental lamellar keratoplasty in rabbits using microfibrilar cellulose membrane: clinical, morphological and immunohistochemical findings

Macedo¹,

Ribeiro

Conceição

et al. 2010

Cienc. Rural

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The clinical, histopathological and immunohistochemical features of the cornea were investigated in adult male New Zealand rabbits submitted to lamellar keratoplasty with microfibrillar cellulose membrane. Thirty animals were divided into five groups (n=6) and evaluated up to 60 days after surgery. Clinical examination revealed moderate manifestations of edema, blepharospasm and photophobia on the second day, which became mild or disappeared after the seventh day. This period was characterized clinically by repair of the corneal defect. Histopathological analysis showed the presence of a thin layer of squamous cells covering the damaged area as early as 7th day, accompanied by a mild infiltrate of polymorphonuclear cells. Blood vessels were observed in the epithelium after the 15th day, which had regressed by day 48. Ki67 antibody labeling showed an increase of proliferating cells in the epithelium by the 15th day and in the stroma by day 30. Remodeling and epithelial adhesion were observed during this period. Microfibrillar cellulose membrane (Bionext®) used for lamellar keratoplasty was found to yield good results considering the good integration of the implant.

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Avaliação Clínica Preliminar Do Fechamento Comparativo De Incisões Corneanas Com Sutura E Biofill (Película Celulósica)

Cited by 8 publications

References 4 publications

Use of a gel biopolymer for the treatment of eviscerated eyes: experimental model in rabbits

Use of a gel biopolymer for the treatment of eviscerated eyes: experimental model in rabbits

The use of xenologous amniotic membrane to repair canine corneal perforation created by penetrating keratectomy

Experimental lamellar keratoplasty in rabbits using microfibrilar cellulose membrane: clinical, morphological and immunohistochemical findings

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