Effects of probiotic bacteria on Candida presence and IgA anti-Candida in the oral cavity of elderly

Abstract

Imbalance in the resident microbiota may promote the growth of opportunistic microorganisms, such as yeasts of Candida genus and the development of diseases, especially in aged people. This study evaluated whether the consumption of the probiotic Yakult LBz(®) (Lactobacillus casei and Bifidobacterium breve) was able to influence on the specific immunological response against Candida and on the presence of these yeasts in the oral cavity of 42 healthy aged individuals. Saliva samples were collected before and after the probiotic use for 30 days, 3 times a week. The samples were plated in Dextrose Saboraud Agar with chloramphenicol, the colony-forming units (CFU/mL) were counted and the Candida species were identified. Anti-Candida IgA analysis was conducted using the ELISA technique. ANOVA and Student's t-test were used for normally distributed data and the Wilcoxon test was used for data with non-normal distribution (α=0.05). The results showed a statistically significant reduction (p<0.05) in Candida prevalence (from 92.9% to 85.7%), in CFU/mL counts of Candida and in the number of non-albicans species after consumption of the probiotic. Immunological analysis demonstrated a significant increase (p<0.05) in anti-Candida IgA levels. In conclusion, probiotic bacteria reduced Candida numbers in the oral cavity of the elderly and increased specific secretory immune response against these yeasts, suggesting its possible use in controlling oral candidosis.

The Inhibitory Effect of Probiotic Bacteria against Drug - Resistant Candida Species Isolated from the Oral Cavity of the Elderly

“…In this study, the saliva samples were collected three times a week for 30 days before and after consuming probiotics. The results showed a remarkable decrease in the prevalence of Candida (from 92.9% to 85.7%) (21). In the present study, the laboratory study on antagonistic effects of isolated probiotic bacteria also showed their inhibitory effect by inhibiting the growth of Candida species.…”

Probiotics as Antifungals in Mucosal Candidiasis

“…A widely available probiotic-laced drink containing Lactobacillus casei and Bifidobacterium breve was able to reduce the prevalence of oral Candida in healthy individuals [39]. A significant increase in anti-Candida immunoglobulin A levels was associated with probiotic consumption [39]. In contrast, the identical product did not significantly affect the oral candidal colonization in complete denture wearers [33] and in healthy dentate people [34], after 4 weeks of administration.…”

“…A widely available probiotic-laced drink containing Lactobacillus casei and Bifidobacterium breve was able to reduce the prevalence of oral Candida in healthy individuals [39]. A significant increase in anti-Candida immunoglobulin A levels was associated with probiotic consumption [39].…”

Lactobacillus rhamnosus pode alterar a virulência de Candida albicans

“…Estudos prévios têm demonstrado que o consumo de alimentos probióticos pode reduzir a quantidade de Candida na microbiota normal dos seres humanos, podendo auxiliar no controle da candidíase 13,14 . Mostrou-se também que o tratamento com fluconazol associado ao Lactobacillus rhamnosus e ao Lactobacillus reuteri, via oral, diminuiu, em quatro semanas, os sintomas relacionados à vulvovaginite por Candida albicans, além de diminuir o crescimento da levedura em cultura 4 .…”