In vitro genotoxicity and cytotoxicity in murine fibroblasts exposed to EDTA, NaOCl, MTAD and citric acid

Abstract

The aim of the present study was to evaluate the capacity of some root canal irrigants to induce genetic damage and/or cellular death in vitro. Murine fibroblast cells were exposed to ethylenediaminetetraacetic acid (EDTA), sodium hypochlorite (NaOCl), MTAD™ and citric acid in increasing concentrations for 3 h at 37ºC. The negative control group was treated with vehicle control (phosphate buffer solution - PBS) for 3 h at 37°C, and the positive control group was treated with methylmetanesulfonate, 1 μM. for 3 h at 37°C. Cytotoxicity was assessed by the trypan blue test and genotoxicity was evaluated by the single cell gel (comet) assay. The results showed that exposure to 2.5% and 5% NaOCl and 8.5% citric acid resulted in a significant cytotoxic effect. NaOCl, EDTA and citric acid did not produce genotoxic effects with respect to the comet assay data for all evaluated concentrations. Although MTAD was not a cytotoxic agent, it showed significant genotoxic effects at all tested concentrations (ANOVA and Tukey's test; p<0.05). NaOCl, EDTA and citric acid were found to be cytotoxic in a dose-dependent manner, but they were not genotoxic. MTAD did not cause cell death, but presented genotoxic effects.

Toxicity of irrigating solutions and pharmacological associations used in pulpectomy of primary teeth

“…Marins et al . () also found no DNA damage with NaOCl (1.25%, 2.5% and 5%) in the alkaline comet assay, but the exposure time was lower (3 h).…”

Evaluation of the effects of the solution used for electrochemical dissolution of nickel–titanium endodontic files on dentine structure, microhardness and cell viability

“…SEM has been widely used to study methods for smear layer removal (Marins et al . , Castagna et al . , Martins Justo et al .…”

Características microbiológicas, patogenicidade e viabilidade do Entercoccus faecalis e seu cultivo in vitro em pesquisas microbiológicas na área da Endodontia

“…No entanto, é uma solução citotóxica, quando utilizada em elevadas concentrações 43 , instável quimicamente 44 e interfere negativamente na adesão do material restaurador à dentina 45 . Além disso, com o advento dos sistemas reciprocantes, a instrumentação tornou-se mais rápida, reduzindo o tempo de contato das soluções de hipoclorito com as paredes do canal radicular, podendo interferir no processo de descontaminação, especialmente quando são utilizadas baixas concentrações de NaOCl, no intuito de reduzir a sua citotoxicidade.…”