Cytotoxicity of bovine and porcine collagen membranes in mononuclear cells

Abstract

This study compared the cytotoxicity and the release of nitric oxide induced by collagen membranes in human mononuclear cells. Peripheral blood was collected from each patient and the separation of mononuclear cells was performed by Ficoll. Then, 2x10(5) cells were plated in 48-well culture plates under the membranes in triplicate. The polystyrene surface was used as negative control. Cell viability was assessed by measuring mitochondrial activity (MTT) at 4, 12 and 24 h, with dosage levels of nitrite by the Griess method for the same periods. Data had non-normal distribution and were analyzed by the Kruskal-Wallis test (p<0.05). Statistically significant differences (p<0.05) were observed between the membranes and the control in the experimental period, although there was a significant reduction in viability over time (p<0.01). At 4 and 12 h, the porcine membrane induced a higher release of nitrite compared with the control and bovine membrane, respectively (p<0.01), and this difference was maintained at 24 h (p<0.05). This in vitro study showed that the porcine collagen membrane induces an increased production of proinflammatory mediators by mononuclear cells in the first hours of contact, decreasing with time.

Fabrication and Characterization of Chitosan-Collagen Membrane from Barramundi (Lates Calcarifer) Scales for Guided Tissue Regeneration

“…31 The mechanisms of GTR/GBR are to isolate periodontal bone defects from gingival connective tissue so that the new bone growth will be formed along with the alveolar bone defect. 32 33 Barrier membrane with a favorable function has to meet certain important design criterion that is biocompatibility. It should not stimulate the immune system or produce sensitization that may interfere with the wound healing process.…”

Non-cross-linked collagen type I/III materials enhance cell proliferation: in vitro and in vivo evidence

“…Mononuclear cells were incubated with collagen membranes of porcine or bovine origin up to 48 hours, and, subsequently, the cytotoxic potential of the membranes was evaluated using the MTT assay 17 . It was shown that all collagen membranes, and in particular those of porcine origin, caused an increased production of pro-inflammatory mediators in the mononuclear cells and a decreased cellular proliferation.…”

The Condensation of Collagen Leads to an Extended Standing Time and a Decreased Pro-inflammatory Tissue Response to a Newly Developed Pericardium-based Barrier Membrane for Guided Bone Regeneration

“…Each of the two study groups contained 20 experimental animals and 5 animals were used for implantation of the respective biomaterial per time point (n=5), i.e., 10, 30, 60 and 90 days. The implantation was conducted following the protocol described by 28,30). Initially, the animals were anesthetized via an intraperitoneal injection of 10 ml ketamine (50 mg/ml) and 1.6 ml xylazine (2%) and their dorsal skin within the subscapular region was shaved and disinfected.…”

“…Furthermore, four additional sections of every tissue explant were used for immunohistochemical detection of macrophages and their M1-and M2-subtypes by means of antibodies against pro-and antiinflammatory molecules, i.e., hemoglobin scavenger receptor (CD163) and mannose receptor (MR, also known as CD206) based on previously published methods (28,(30)(31)(32). Briefly, the slides were initially treated with citrate buffer and proteinase K at pH 8 for 20 min in a water bath at 96˚C followed by equilibration using TBS-T buffer.…”

“…In the case of natural collagen based materials, it has been assumed that macrophages are integrated into the "natural metabolism" process, together with fibroblasts and eosinophils (19). Thus, collagen materials that induce a tissue reaction involving the afore-mentioned mononuclear cell types, are assumed to be mostly biocompatible (7,30). However, collagen materials can also induce a tissue response involving biomaterialassociated multinucleated giant cells (BMGCs) (31).…”