Bacteriostatic effect of copaiba oil (Copaifera officinalis) against Streptococcus mutans

Pieri, Fábio Alessandro; Mussi, Maria Carolina Martins; Fiorini, João Evangelista; Moreira, Maria Aparecida Scatamburlo; Schneedorf, José Maurício

doi:10.1590/s0103-64402012000100006

Cited by 32 publications

(35 citation statements)

References 14 publications

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“…In a review of the fundamentals about black stains, its diagnosis and possible differential diagnoses, microbiology and therapy, Ronay and Attin (15) concluded that the reasons why the results are not clear about the black stains are related to the specific oral microflora of these stains. Another study (22) reinforced that chlorhexidine solution could cause some stains on teeth surface and confirms the great potential of using copaiba oil and chlorhexidine solution against the growth of S. mutans. The present study demonstrated that black pigmented bacteria (P. nigrescens and P. intermedia) as well as cariogenic non-black pigmented bacteria (S. mutans and Actinomyces spp) can be detected in dental biofilms of patients carrying black tooth stains or not.…”

Section: Discussionmentioning

confidence: 62%

Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans

et al. 2012

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This study investigated the presence of the black-pigmented bacteria Prevotella nigrescens and Prevotella intermedia, the non-black-pigmented bacteria Actinomyces spp and particularly the cariogenic pathogen Streptococcus mutans in the dental biofilms of patients with or without black extrinsic tooth stains, using the multiplex polymerase chain reaction (PCR) technique. Analysis of the dental biofilms of patients with (n=26) or without (n=26) black tooth stains was performed using duplex PCR for the 16S ribosomal RNA gene (P. nigrescens, P. intermedia, Actinomyces spp) and glucosyltransferase-I gene for S. mutans. P. nigrescens and S. mutans were the most frequent bacteria detected in both groups. The least frequently detected were P. intermedia and Actinomyces spp. The similar bacterial composition of dental biofilms of black tooth stains and healthy tooth surfaces indicates that black tooth stains are not free of cariogenic bacteria.

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Section: Discussionmentioning

confidence: 62%

Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans

et al. 2012

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“…Used since the 16th century for medicinal purposes 2,6 , Copaíba oil is described in the literature as anti-edematous 5 , anti-inflammatory 3,7,8 , antibacterial 8,9 , insecticide 5,6,8 , antifungal 10 and wound healing 8,11 . It is popularly adopted in the treatment of several diseases, such as cystitis, bronchitis, chronic diarrhea, rheumatism, psoriasis, tumors 9 and gonorrhea 4 .…”

Section: Officinaliis C Reticulata C Langsdorffii C Guyanensismentioning

confidence: 99%

Comparative analysis of the effects of Copaifera multijuga oil-resin and nitrofurazona in the cutaneous wound healing process

Martini

Scapini

Collaço

et al. 2016

Rev. Col. Bras. Cir.

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Objectives: to evaluate, histologically and macroscopically, the influence of Copaifera multijuga (Copaíba) oil-resin on the healing process of cutaneous wounds, comparing it with nitrofurazone. Methods: we divided 36 rats into three groups of 12 animals, according to the treatment to be administered. Group SL (control) received saline on the lesion; the Group OIL received topical treatment with Copaíba oil; and the Group NITRO was treated with Nitrofurazone. We inflicted a circular wound of 8mm in diameter on the back of each animal. We subdivided each of the three groups of 12 animals into three subgroups, according to treatment time and euthanasia (7, 14 and 21 days). All animals received the proposed treatment daily. We photographed the lesions for area measurement, as well as for evaluation of macroscopic aspects. We resected and stained the scars to quantify and qualify elastic fibers, collagen, degree of epithelization, neovascularization and inflammation. Results: although the saline solution provided a faster wound closure in its initial phase, after 14 days the wound size of the three groups tested was the same. Levels of inflammation and neovascularization were similar in all three groups. The amount of collagen and elastic fibers was higher in the Nitrofurazone and Copaíba oil groups. Conclusion: in male Wistar rats, Copaifera multijuga oil-resin positively influences the healing process, but it is less effective than nitrofurazone in healing by secondary intention.

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“…Mutans streptococci and lactobacilli are microorganisms with known cariogenic capacities (6)(7)(8). The first is considered an etiological agent of carious lesions and is able to produce (acidogenic) and tolerate (aciduric) high quantities of lactic acid, and the second is considered particularly involved in the progression of carious lesions (8,9).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity and Synergism of Lactoferrin and Lysozyme Against Cariogenic Microorganisms

Andrade

Oliveira²,

Yoshie³

et al. 2014

Braz. Dent. J.

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The present study evaluated the antimicrobial in vitro effects of the salivary proteins lactoferrin and lysozyme on microorganisms involved in the carious process, obtaining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Streptococcus mutans (ATCC 25175) and Lactobacillus casei (ATCC 7469) were submitted to broth macrodilution of lysozyme at 80 mg/mL and lactoferrin at 200 mg/mL. The tubes were read in a spectrophotometer after they had been incubated at 37 °C for 18 h, in a carbon dioxide chamber, in order to read the MIC. A new subculture was carried on agar plates to obtain the MBC. The agar diffusion method was also tested, using BHI agar with 100 µL of the standardized microbial inocula. Filter-paper disks soaked in 10 µL of the solutions lactoferrin (200 µg/mL) and lysozyme (80 µg/mL) were placed on the agar surface. Inhibition halos were not observed on the plates, showing the absence of the antimicrobial effects of these proteins in this method. The bactericidal and bacteriostatic effects of lysozyme on L. casei were 50.3 mg/mL and 43.1 mg/mL respectively. The bactericidal and bacteriostatic effects on S. mutans were 68.5 mg/mL and 58.7 mg/mL. Lactoferrin did not induce any inhibitory effects on any microorganism, even in the concentration of 200 mg/mL. There was not a synergic antimicrobial effect of proteins, when they were tested together, even in the concentration of 42.8 mg/mL of lysozyme and 114 mg/mL of lactoferrin (the highest values evaluated). S. mutans and L. casei were only inhibited by lysozyme, not affected by lactoferrin and by the synergic use of both proteins.

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Bacteriostatic effect of copaiba oil (Copaifera officinalis) against Streptococcus mutans

Cited by 32 publications

References 14 publications

Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans

Biofilms of black tooth stains: PCR analysis reveals presence of Streptococcus mutans

Comparative analysis of the effects of Copaifera multijuga oil-resin and nitrofurazona in the cutaneous wound healing process

Antimicrobial Activity and Synergism of Lactoferrin and Lysozyme Against Cariogenic Microorganisms

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