Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth

García, E. Navarro; Oldoni, Tatiane Luiza Cadorin; Alencar, Severino Matias de; Reis, Alessandra; Loguércio, Alessandro Dourado; Grande, Rosa Helena Miranda

doi:10.1590/s0103-64402012000100004

Cited by 264 publications

(200 citation statements)

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“…10 Fractions obtained from both extracts showed potential antioxidant activity may due to the presence of higher amount of alkaloids, flavonoids and phenols based on qualitative phytochemical screening results. Medini et al (2014) reported that antioxidant capacity is highly associated with phenolic content and most of the radical scavenging activity of plants is derived from phenols.…”

Section: Discussionmentioning

confidence: 96%

Phytochemical Screening and Evaluation of Cytotoxic Effect and Antioxidant Activity of Fractions Isolated from Stenochlaena palustri (Burm.f.) Bedd. Leaves

Arullappan¹,

Sawai²,

Chee³

et al. 2017

IJPER

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Introduction: Stenochlaena palustris (Burm.f.) Bedd. is an edible fern from Blechnaceae family, native to India through Southeast Asia to Polynesia and Australia. The study was conducted to evaluate the cytotoxic effect and antioxidant activity of fractions obtained from Stenochlaena palustris leaf extracts. Methods: Stenochlaena palustris (Burm.f.) Bedd. was tested for its antioxidant activity using DPPH assay and in vitro cytotoxic effect against HeLa cancer cell line using MTT assay. Major fractions were obtained from ethanol and ethyl acetate leaves extract of Stenochlaena palustris (Burm.f.) Bedd. through gravity column chromatography and the secondary metabolites were screened using qualitative phytochemical analysis. Results: In DPPH assay, the highest radical scavenging activity was exhibited by fraction 11 of ethanol extract and fraction 4 of ethyl acetate extract at 98.47±0.002% (ED 50 =0.120 mg/mL), and 81.38±0.018% (ED 50 =0.650 mg/mL), respectively. Meanwhile, ascorbic acid and kaempferol exhibited radical scavenging of 80.95±0.002% (ED 50 =0.014 mg/mL) and 98.67±0.006% (ED 50 =0.011 mg/mL). As for MTT assay, the percentages of cell viability of both cell lines decreased as the concentration increased. Fraction 7 of ethanol extract and fraction 1 of ethyl acetate extract exhibited the lowest IC 50 value of 4.58 µg/mL and 8.60 µg/ml, respectively. Doxorubicin hydrochloride showed lowest IC 50 value of 2.21 µg/mL against HeLa cells. Conclusion: The fractions isolated from ethanol and ethyl acetate leaves extract of Stenochlaena palustris (Burm.f.) Bedd. exhibited higher cytotoxic effect against HeLa cells, and higher radical scavenging activity.

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Section: Discussionmentioning

confidence: 96%

Phytochemical Screening and Evaluation of Cytotoxic Effect and Antioxidant Activity of Fractions Isolated from Stenochlaena palustri (Burm.f.) Bedd. Leaves

Arullappan¹,

Sawai²,

Chee³

et al. 2017

IJPER

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“…DPPH free radical scavenging assay is the simplest method for evaluating the antioxidant potential of an extract [24] , which is based on the electron-transfer that produces a violet solution [25] . This free radical, stable at room temperature, is reduced in the presence of an antioxidant molecule, giving rise to a colourless solution [26] . With this method, the determination of antiradical power of an antioxidant is possible by measuring the decrease in absorbance of DPPH at 517 nm [27] .…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Antioxidant Capacity and Identification of Bioactive Compounds of Crude Methanol Extracts of Caesalpinia pulcherrima (L.) Swartz

Torre¹

2017

ijps

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PhilippinesDela Torre, et al.: Antioxidant Capacity of C. pulcherrimaThe study was conducted to determine the antioxidant capacity, expressed as half maximal inhibitory concentration, IC 50 , of Caesalpinia pulcherrima leaf, flower and seed methanol extracts, and their correlation to their total phenolic, flavonoid and triterpenoid contents. Thin layer chromatographic profiling of the methanol extracts was also conducted followed by ultra-performance liquid chromatography quadruple time-of-flight mass spectrometric analysis for the identification of antioxidant compounds. Based on the quantitative antioxidant assays, all extracts exhibited comparable activity with the reference standard at 800 µg/ml (P>0.05). Correlation data revealed a strong negative correlation between the IC 50 and the total phenolic, flavonoid and triterpenoid contents of the extracts, with statistically significant negative correlations observed between the flavonoids of leaf (r=−0.997) and flower (r=−0.998) with reducing power assay, and triterpenoids of flower (r=−1.000) with 2,2-diphenyl-1-picrylhydrazyl scavenging assay. Two common spots with antioxidant activity in the thin layer chromatography profiles were subjected to ultra-performance liquid chromatography quadruple time-of-flight mass spectrometric. The majority of compounds were identified in the library as triterpenoids, flavonoids and phenolics, and still a large quantity of compounds were unidentified. Hyperforin, 3-(4'-hydroxy-benzyl)-5,7-dihydroxy-6,8-dimethyl-chroman-4-one and platycodigenin were identified to be the common compounds present on the three plant parts.

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“…13,14,[16][17][18] In a previous study, 10% ascorbic acid applied for only one minute was effective in increasing the bond strength of bleached teeth. 18 The ionized form of ascorbic acid, namely, sodium ascorbate, was tested in different concentrations and for different time periods, but a recent study 26 suggests that 10% sodium ascorbate was not effective in increasing the bond strength of composites to bleached enamel and/or dentin.…”

Section: Methodsmentioning

confidence: 99%

“…[9][10][11] Recently, some attempts to reduce the immediate inability of adhesion have been made with antioxidant solutions applied to the tooth structure after bleaching procedures. Many antioxidant agents have been tested, such as sodium ascorbate, [12][13][14][15][16][17] ascorbic acid, 17,18 α-tocopherol 12,17 and even a solution of grape seed extract. 19 Their presentation varies from solutions to gels, and application time varies from ten minutes 19 to one hour.…”

Section: Introductionmentioning

confidence: 99%

Effect of Sodium Ascorbate and Ascorbic Acid Hydrogels on Microleakage of Composite Restorations after an Office Bleaching Protocol

et al. 2014

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Aim: This study evaluated the infl uence of 20% sodium ascorbate (SA) and 10% ascorbic acid (AA) hydrogels on the microleakage of class V composite restorations after an offi ce bleaching protocol (B). Methods: Sixty bovine incisors were sectioned at one point extending 8 mm from the amelodentinal junction toward the incisal edge, and at a second point extending 2 mm from the amelodentinal junction toward the root apex. Root canals were sealed with a composite and the teeth were divided into 5 groups. In Group C (control group), the teeth were prepared (P; 4 × 3 mm depth × diameter; diamond bur 3131, KG Sorensen), etched, rinsed (ER), submitted to an adhesive system protocol (Ambar, FGM), and restored (R) with a microhybrid composite (Opallis, FGM); In Group IR, the teeth were bleached (B; 2 sessions, 1 week interval, 35% hydrogen peroxide, Whitness HP Blue, FGM) + P + ER + R; In Group SA, teeth were B + P + SA (20%/15 minutes) + ER + R; In Group AA, teeth were B + P + AA (10%/15 minutes) + ER + R; and in Group MR, the teeth were B + stored in water (37°C/14 days) + P + ER + R. Specimens were submitted to an aging process by thermocycling (5 × 10 4 cycles of 5°C and 55°C with a dwelling time of 30 s), varnish-sealed, immersed in basic fuchsin (3 h), washed and sectioned with a diamond disc. Microleakage was measured with the aid of ImageTool  software. ANOVA and Tukey's post hoc test (p < 0.05) were applied. Results: Microleakage (mm) and standard deviations per group were as follows: C, 0.29 (0.06) A ; IR, 1.86 (0.15) C ; SA, 1.08 (0.09) B ; AA, 1.07 (0.10) B ; MR, 1.02 (0.12) B . Conclusions: Bleached teeth submitted to superfi cial treatment with 20% SA and 10% AA applied for 15 minutes prior to restoration presented reduced microleakage, as compared to immediately restored bleached teeth. Use of these antioxidants led to a microleakage comparable to that observed after a waiting period of 14 days prior to restoration. Dental Materials; Dental Leakage; Tooth Bleaching.Efeito de hidrogéis de ascorbato de sódio e ácido ascórbico na microinfi ltração de restaurações de resina composta realizadas após aplicação de um protocolo de clareamento em consultório • Objetivo: Este estudo avaliou a infl uência da utilização de hidrogéis de ascorbato de sódio (SA) e ácido ascórbico (AA) na microinfi ltração de restaurações classe V de resina composta realizadas após a aplicação de um protocolo de clareamento em consultório (B). Métodos: Sessenta incisivos bovinos foram seccionados a 8 mm da junção amelodentinária em direção incisal e a 2 mm em direção ao ápice radicular. Os canais radiculares foram selados com um compósito e os dentes foram divididos em 5 grupos: no grupo C (controle), os dentes foram preparados (P; 4 × 3 mm profundidade × diâmetro; ponta diamantada 3131, KG Sorensen), condicionados, lavados (ER), submetidos a um protocolo adesivo (Ambar, FGM) e restaurados (R) com uma resina composta microhíbrida (Opallis, FGM); no grupo IR, os dentes foram clareados (B; 2 sessões, 1 semana de intervalo, peróxido de hidr...

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Antioxidant activity by DPPH assay of potential solutions to be applied on bleached teeth

Cited by 264 publications

References 20 publications

Phytochemical Screening and Evaluation of Cytotoxic Effect and Antioxidant Activity of Fractions Isolated from Stenochlaena palustri (Burm.f.) Bedd. Leaves

Phytochemical Screening and Evaluation of Cytotoxic Effect and Antioxidant Activity of Fractions Isolated from Stenochlaena palustri (Burm.f.) Bedd. Leaves

Evaluation of Antioxidant Capacity and Identification of Bioactive Compounds of Crude Methanol Extracts of Caesalpinia pulcherrima (L.) Swartz

Effect of Sodium Ascorbate and Ascorbic Acid Hydrogels on Microleakage of Composite Restorations after an Office Bleaching Protocol

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