Genotypic and phenotypic analysis of S. mutans isolated from dental biofilms formed in vivo under high cariogenic conditions

Arthur, Rodrigo Alex; Cury, Altair Antoninha Del Bel; Mattos-Graner, Renata O.; Rosalen, Pedro Luiz; Vale, Gláuber Campos; Leme, Adriana Franco Paes; Cury, Jaime Aparecido; Tabchoury, Cínthia Pereira Machado

doi:10.1590/s0103-64402011000400001

Cited by 18 publications

(33 citation statements)

References 17 publications

(25 reference statements)

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“…A 100 bp DNA ladder was included as a molecular-size marker in each gel. All PCR reagents were obtained from Invitrogen, Life Technologies, São Paulo, Brazil [16, 17]. …”

Section: Methodsmentioning

confidence: 99%

Genotypic diversity and phenotypic traits of Streptococcus mutans isolates and their relation to severity of early childhood caries

et al. 2017

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BackgroundEarly childhood caries (ECC) is an aggressive condition that can affect teeth of young children. This study aimed to evaluate genotypic diversity and phenotypic traits of S. mutans isolated from dental biofilms of children with different caries status in comparison with caries free (CF) children.Methods Streptococcus mutans strains were isolated from supragingival biofilm samples of CF, ECC and severe-ECC (S-ECC) children and genotyped by arbitrary-primer polymerase chain reaction - AP-PCR. S. mutans genotypes were tested for their ability to reduce the suspension pH through glycolysis, to tolerate extreme acid challenge and by their ability to form biofilm. Response variables were analyzed by ANOVA/Tukey or Kruskal-Wallis/Mann-Whitney tests at a 5% of significance.ResultsThere was an increase in the prevalence of Streptococcus mutans in biofilms with the severity of dental caries. No differences in genotypic diversity and in acidogenicity of genotypes were found among CF, ECC and S-ECC children. S mutans strains with genotypes more characteristic for ECC and S-ECC children formed more biofilms than those identified in CF children. The strains isolated from S-ECC children were highly acid tolerant.ConclusionAlthough S. mutans genotypic diversity was similar among the groups of children, phenotypic traits of S. mutans, especially the acid tolerance response, could explain the severity of early childhood caries.

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“…A 100 bp DNA ladder was included as a molecular-size marker in each gel. All PCR reagents were obtained from Invitrogen, Life Technologies, São Paulo, Brazil [16, 17]. …”

Section: Methodsmentioning

confidence: 99%

Genotypic diversity and phenotypic traits of Streptococcus mutans isolates and their relation to severity of early childhood caries

et al. 2017

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“…Mutans streptococci and lactobacilli are microorganisms with known cariogenic capacities (6)(7)(8). The first is considered an etiological agent of carious lesions and is able to produce (acidogenic) and tolerate (aciduric) high quantities of lactic acid, and the second is considered particularly involved in the progression of carious lesions (8,9).…”

Section: Introductionmentioning

confidence: 99%

“…The first is considered an etiological agent of carious lesions and is able to produce (acidogenic) and tolerate (aciduric) high quantities of lactic acid, and the second is considered particularly involved in the progression of carious lesions (8,9). Mutans streptococci and lactobacilli have been correlated positively with caries prevalence and caries increment and the existence of saliva-caries relationships have been reported (1,(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity and Synergism of Lactoferrin and Lysozyme Against Cariogenic Microorganisms

Andrade

Oliveira²,

Yoshie³

et al. 2014

Braz. Dent. J.

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The present study evaluated the antimicrobial in vitro effects of the salivary proteins lactoferrin and lysozyme on microorganisms involved in the carious process, obtaining their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Streptococcus mutans (ATCC 25175) and Lactobacillus casei (ATCC 7469) were submitted to broth macrodilution of lysozyme at 80 mg/mL and lactoferrin at 200 mg/mL. The tubes were read in a spectrophotometer after they had been incubated at 37 °C for 18 h, in a carbon dioxide chamber, in order to read the MIC. A new subculture was carried on agar plates to obtain the MBC. The agar diffusion method was also tested, using BHI agar with 100 µL of the standardized microbial inocula. Filter-paper disks soaked in 10 µL of the solutions lactoferrin (200 µg/mL) and lysozyme (80 µg/mL) were placed on the agar surface. Inhibition halos were not observed on the plates, showing the absence of the antimicrobial effects of these proteins in this method. The bactericidal and bacteriostatic effects of lysozyme on L. casei were 50.3 mg/mL and 43.1 mg/mL respectively. The bactericidal and bacteriostatic effects on S. mutans were 68.5 mg/mL and 58.7 mg/mL. Lactoferrin did not induce any inhibitory effects on any microorganism, even in the concentration of 200 mg/mL. There was not a synergic antimicrobial effect of proteins, when they were tested together, even in the concentration of 42.8 mg/mL of lysozyme and 114 mg/mL of lactoferrin (the highest values evaluated). S. mutans and L. casei were only inhibited by lysozyme, not affected by lactoferrin and by the synergic use of both proteins.

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“…However most of them have focused just on sucrose and/or glucose [12][13][14][20][21][22][23][24] . At present, little is known about the role of maltose and lactose.…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary sugars on dual-species biofilms of Streptococcus mutans and Streptococcus sobrinus – a pilot study

Oliveira

Albuquerque

Spolidório

et al. 2016

Rev. odontol. UNESP

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ResumoIntrodução: O consumo frequente de açucares e a presença de Streptococcus mutans e Streptococcus sobrinus estão correlacionados com maior experiência de cárie. Objetivo: Elucidar o efeito de diferentes carboidratos fermentáveis na biomassa e acidogenicidade de biofilmes formados por S. mutans e S. sobrinus. Material e método: Biofilmes única e dupla-espécie de S. mutans ATCC 25175 e S. sobrinus ATCC 27607 em concentrações iguais cresceram no fundo de placas de microtitulação por 24 h a 37 °C em microaerofilia. Maltose, sacarose, glicose e lactose foram adicionados a 2%. BHI caldo (0.2% glicose) foi usado como controle negativo. Acidogenicidade foi avaliada por meio da medição do pH do meio de cultura após 24 h, imediatamente após troca de meio e nas próximas 1 h e 2 h. Coloração por cristal violeta foi usada como indicador do total de biomassa aderida, após 24 h de incubação. Os dados foram analisados por teste ANOVA two way e Teste de Bonferroni. O nível de significância foi de 5%. Resultado: Todos os carboidratos resultaram em maior formação de biomassa em ambos os tipos de biofilme (única ou dupla-espécie), quando comparado ao grupo controle. Sacarose, lactose e maltose mostraram maior acidogenicidade que o grupo controle após 24 h nos biofilmes única ou dupla-espécie, apenas após 24 h. Conclusão: Os achados indicam que o tipo de biofilme (única ou dupla-espécie) e o tipo de carboidrato usado podem influenciar tanto na quantidade de biomassa formada quanto na taxa de redução do pH.Descritores: Biofilme; biomassa; Streptococcus mutans; Streptococcus sobrinus. AbstractIntroduction: Frequent consumption of sugars and the presence of Streptococcus mutans and Streptococcus sobrinus are correlated with higher caries experience. Objective: The aim of this pilot study was to elucidate the effect of different fermentable carbohydrates on biomass formation and acidogenicity of S. mutans and S. sobrinus biofilms. Material and method: Single and dual-species biofilms of S. mutans ATCC 25175 and S. sobrinus ATCC 27607 were grown at the bottom of microtiter plates at equal concentrations for 24 h at 37 °C under micro-aerobic atmosphere. Carbohydrates were added at 2% concentration: maltose, sucrose, glucose and lactose. BHI Broth (0.2% glucose) was used as negative control. Acidogenicity was assessed by measuring the pH of spent culture medium after 24 h, immediately after refreshing the culture medium and for the next 1 h and 2 h. Crystal violet staining was used as an indicator of the total attached biofilm biomass after 24 h incubation. Data were analyzed by two-way ANOVA followed by Bonferroni post hoc test. Significance level was set at 5%. Result: All carbohydrates resulted in higher biomass formation in single-and dual-species biofilms when compared to the control group. Sucrose, lactose and maltose showed higher acidogenicity than the control group in both single-and dual-species biofilms after 24 h. Conclusion: These findings indicate that the type of biofilm (single-or dual-species) and the carbohydrate use...

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Genotypic and phenotypic analysis of S. mutans isolated from dental biofilms formed in vivo under high cariogenic conditions

Cited by 18 publications

References 17 publications

Genotypic diversity and phenotypic traits of Streptococcus mutans isolates and their relation to severity of early childhood caries

Genotypic diversity and phenotypic traits of Streptococcus mutans isolates and their relation to severity of early childhood caries

Antimicrobial Activity and Synergism of Lactoferrin and Lysozyme Against Cariogenic Microorganisms

Effect of dietary sugars on dual-species biofilms of Streptococcus mutans and Streptococcus sobrinus – a pilot study

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