Characterization and in vitro cytocompatibility of an acid-etched titanium surface

Abstract

The aims of this study were to characterize the microstructure of a commercially pure titanium (cpTi) surface etched with HCl/H2SO4 (AE-cpTi) and to investigate its in vitro cytocompatibility compared to turned cpTi (T-cpTi). T-cpTi showed a grooved surface and AE-cpTi revealed a surface characterized by the presence of micropits. Surface parameters indicated that the AE-cpTi surface is more isotropic and present a greater area compared to T-cpTi. The oxide film thickness was similar between both surfaces; however, AE-cpTi presented more Ti and O and less C. Osteoblastic cell proliferation, alkaline phosphatase activity, and bone-like nodule formation were greater on T-cpTi than on AE-cpTi. These results show that acid etching treatment produced a surface with different topographical and chemical features compared to the turned one, and such surface modification affected negatively the in vitro cytocompatibility of cpTi as demonstrated by decreasing culture growth and expression of osteoblastic phenotype.

Influence of different acid etchings on the superficial characteristics of Ti sandblasted with Al2O3

“…Each test group was acid-etched by five different acid etchings (defined as groups AT1 to AT5) previously described in the literature [16][17][18][19][20] . Table 1 presents a detailed description of these 5 acid etching treatments.…”

Electrochemical construction of a bio-inspired micro/nano-textured structure with cell-sized microhole arrays on biomedical titanium to enhance bioactivity

“…However, up to now, most of the microscale structures of the hierarchical micro/nano-textured surfaces, fabricated by acid etching [11,22,24,25], sand-blasting [26], electro-eroding [27][28][29] or some combination of them [30], have usually been unordered and irregular and may be adverse to the further improvement of their bioactivity [12,31,32]. An unordered structure will lead to insufficient synergistic functions in the interaction between cells and biomaterials [9].…”

Study of the influence of acid etching treatments on the superficial characteristics of Ti

“…Each test group was acid-etched by a different acid treatment (defined as groups AT1 to AT5) previously described in the literature [14][15][16][17][18] . Table 1 presents a detailed description of these 5 acid-etching treatments.…”