Lack of DNA damage induced by fluoride on mouse lymphoma and human fibroblast cells by single cell gel (comet) assay

Abstract

Fluoride has widely been used in Dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury on genetic apparatus. Genotoxicity tests constitute an important part of cancer research for risk assessment of potential carcinogens. In this study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Mouse lymphoma and human fibroblast cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 μg/mL for 3 h at 37 o C. The results pointed out that NaF in all tested concentrations did not contribute to DNA damage as depicted by the mean tail moment and tail intensity for both cellular types assessed. These findings are clinically important because they represent a valuable contribution for evaluation of the potential health risk associated with exposure to agents usually used in dental practice.

Comparative study of a new composite biomaterial fluor-hydroxyapatite on fibroblast cell line NIH-3T3 by direct test

“…Furthermore, fluoride has been widely used in dentistry as caries prophylactic agents (Ribeiro et al 2004). NaF was the first and still most recommended fluoride compound for fluoridation of drinking water (Ribeiro et al 2006). Fluoride has shown considerable variation in respect of cytotoxicity against different cultured cells (Hongslo et al 2004;Oguro et al 1983).…”

Sodium Fluoride Promotes Apoptosis by Generation of Reactive Oxygen Species in Human Lymphocytes

“…Earlier reports regarding the genotoxic potential of F during in vitro (Anuradha et al, 2001;Ha et al, 2004;Liu et al, 2007;Machalinska et al, 2001) and in vivo (He and Chen, 2006) studies in humans (Gadhia and Joseph, 1997) and rodents (Podder et al, 2008(Podder et al, , 2011 are conflicting. Some studies demonstrated that F did not produce damage to the genetic material (Li et al, 1987;Ribeiro et al, 2006;Ribeiro et al, 2007;Skare et al, 1986). Other studies on oxidative stress in fluorotic humans and F-intoxicated animals indicated that generation of reactive oxygen species (ROS) and lipid peroxidation may be directly induced by F (Ailani et al, 2009;Inkielewicz et al, 2006;Podder et al, 2008;Susheela and Bhatnagar 2002).…”

Antiproliferative, proapoptotic and morphogenic effects of the flavonoid rutin on human glioblastoma cells

“…DNA integrity and single-strand breaks were monitored using single cell gel electrophoresis (comet assay) performed under alkaline conditions, essentially following the procedure described by Ribeiro (2006) with some modifications. Briefly, all control and treated cells seeded on 40 mm polystyrene culture dishes (1.5 Â 10 5 cells/plate) were incubated for 24 or 72 h in the presence of rutin, using 0.5% DMSO as a negative control or 1 h direct exposure to UV light as a positive control.…”