In vitro evaluation of apical sealing in root apex treated with demineralization agents and retrofiled with mineral trioxide aggregate through marginal dye leakage

Abstract

The purpose of this study was to evaluate the apical seal in root apex treated with different demineralization agents and retrofilled with mineral trioxide aggregate (MTA) using marginal dye leakage. Fifty-six, human single-rooted teeth were instrumented, filled, resected and had retrofilling cavities prepared with ultrasonic tips. Demineralizing agents were applied before the apical cavities were retrofilled with Pro Root MTA. The specimens were assigned to 4 groups (n=14), as follows: group 1 (no demineralizing agent); group 2 (35% phosphoric acid, for 15 s); group 3 (17% EDTA solution, pH 7, for 3 min); and group 4 (24% EDTA gel, pH 7, for 4 min). The extension of dye (2% rhodamine B, at 37°C, for 24 h) penetration was measured in millimeters using a stereomicroscope. Results were statistically analyzed by ANOVA and Tukey's test at 5% significance level. Among the experimental groups, the least extension of dye penetration was observed in group 1 (1.89 mm), followed by groups 2 (2.18 mm), 4 (2.54 mm) and 3 (2.64 mm). No statistically significant differences (p<0.05) were found in marginal microleakage among groups 1, 2 and 4 and groups 2, 3 and 4. Based on the results obtained in this study, it may be concluded that the application of demineralizing agents cannot be recommended when MTA is used in periradicular surgeries.

The effect of root dentin conditioning protocols on the push-out bond strength of three calcium silicate sealers

“…Irrigation with EDTA also appears to decrease the sealing efficacy of MTA, 24 h after placement. Kubo et al [25] and Yildirim et al [26] demonstrated that final irrigation with EDTA decreased the sealing ability of MTA. The reduction in bond strength of MTA when a commercial EDTA gel (Glyde Prep Gel) was used has also been reported [27].…”

Mineral Trioxide Aggregate: A Comprehensive Literature Review—Part II: Leakage and Biocompatibility Investigations

“…Dye Leakage. Numerous dye leakage studies have been performed on MTA when used as a root-end filling material (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20). Various types of dyes have been used to evaluate MTA's sealing ability, including methylene blue, fuchsin, rhodamine B, silver nitrate, India ink, and Pelikan ink.…”

“…Different factors influence MTA leakage. They include thickness of the dentinal wall (18), the dye pH (19), the type of dye (12,14,20), pretreatment with chelating agents (13), the tooth storage environment before the experiment (15), and the setting status of MTA before its placement in the dye (16). Fluid Filtration.…”

The effect of endodontic materials on the optical density of dyes used in marginal leakage studies

“…Kubo et al 9 (2005) evaluated the seal of root apexes retrofilled with Pro Root for marginal leakage with RB aqueous solution (pH 7) and dye penetrated 1.89 mm. In previous studies, 6,8,21 it was observed that the dye solution penetrated from 0.28 to 0.31 mm.…”