Distribution of CD8 and CD20 lymphocytes in chronic periapical inflammatory lesions

Philippi, Christine Kalvelage; Rados, Pantelis Varvaki; Filho, Manoel Sant’Ana; Barbachan, João Jorge Diniz; Quadros, Onofre Francisco de

doi:10.1590/s0103-64402003000300008

Cited by 10 publications

(11 citation statements)

References 16 publications

(15 reference statements)

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“…The humoral immune response, mediated by B lymphocytes, is the main immune mechanism against extracellular microorganisms and their toxins, since antibodies bind to them to facilitate their removal. Cellular immunity, mediated by T lymphocytes, promotes the destruction of cells infected by intracellular microorganisms, as the antibodies are unable to attracting these antigens (Philippi et al , 2003). T and B lymphocytes are programmed to respond to specific antigens, and frequently, T lymphocytes consistently exceed B cell infiltration in periapical lesions.…”

Section: Discussionmentioning

confidence: 99%

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Immunohistochemical correlation between microvessel density and lymphoid infiltrate in radicular cysts

Zizzi¹,

Ferrante³

et al. 2012

Oral Diseases

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Oral Diseases (2012) 19, 92–99 Objective: Radicular cysts occur as a result of the immunological response to continuous antigenic stimulation from root canals. We correlated the immunophenotypical composition of the lymphoid infiltrate to the microvessel density expressed by the count of CD34 reactive endothelial cells in radicular cysts. Subjects and methods: Thirty‐four cases of radicular cysts were evaluated by immunohistochemistry, using antibodies against B‐ and T‐cell antigens (CD20, CD3, CD4, CD8) and against the endothelial cell marker CD34. Statistical analysis was performed. Results: In the epithelium, we observed a low amount of lymphoid infiltrate in all 34 radicular cysts, and a strong significant negative correlation between T and B lymphocytes and between T‐helper and T‐cytotoxic/suppressor lymphocytes. In the cyst capsule, we observed a significant positive correlation between B and T lymphocytes, B and T‐cytotoxic/suppressor lymphocytes, T and T‐helper lymphocytes and between the number of CD34+ blood vessels and T and T‐helper lymphocytes, respectively. We observed a statistically significant correlation between percentage of CD34+ vessels and inflammatory infiltrate grade. Conclusions: Both humoral and cellular immune reactions and neovascularization are likely to occur in the complex events of tissue destruction. The inflammatory infiltrate has an important role in neoangiogenesis and consequently in radicular cysts development and growth.

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Section: Discussionmentioning

confidence: 99%

“…Moreover, in agreement with other authors (Liapatas et al , 2003; Philippi et al , 2003), we found that the number of T‐helper lymphocytes was similar or slightly increased compared to that of T‐suppressor/cytotoxic cells, both in the epithelium and in the fibrous capsule.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical correlation between microvessel density and lymphoid infiltrate in radicular cysts

Zizzi¹,

Ferrante³

et al. 2012

Oral Diseases

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“…Evaluation of the intensity of the inflammatory infiltrate showed a larger median number of CD8 + T lymphocytes in lesions with a grade III infiltrate (79.95) compared to those with a grade I/II infiltrate (62.0), but the difference was not statistically significant (P=0.725). According to Philippi et al, 12 the concentration and distribution of CD8 + T lymphocytes are directly associated with the concentration of antigens in periapical lesions. Muglali et al 32 emphasized the importance of proinflammatory cytokines such as IL-1 and TNF-α for the pathogenesis of periapical lesions, including the activation of osteoclasts, production and secretion of matrix metalloproteinases and stimulation of keratinocyte proliferation, as well as promotion of inflammatory cells recruitment.…”

Section: Cd8mentioning

confidence: 99%

“…These findings also suggest an important role of the adaptive immunity in the pathogenesis of chronic periapical lesions. 12,13,14,15 We emphasize that the integrity of the immune system is essential for the defense against infectious organisms and their toxic products. Immunodeficiency can compromise the number and activation of NK cells and CD8 + T lymphocytes, resulting in defects in the effector mechanisms of innate and adaptive immunity, thus leading to increased susceptibility for and difficulty in controlling infections.…”

mentioning

confidence: 99%

Analysis of CD57+ natural killer cells and CD8+ T lymphocytes in periapical granulomas and radicular cysts

et al. 2017

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The aim of this study was to compare the number of CD57 + natural killer (NK) cells and CD8 + T lymphocytes between periapical granulomas (PGs) and radicular cysts (RCs). Twenty-fives cases of PGs and 25 of RCs were submitted to histological analysis and immunohistochemistry using anti-CD57 and anti-CD8 biomarkers. Positive cells were counted in 10 fields (400× magnification) and the median value was calculated for each case. Statistical tests were used to evaluate differences in the number of CD57+ NK cells and CD8 + T lymphocytes according to type of lesion, intensity of the infiltrate and thickness of the lining epithelium. The number of CD57 + NK cells and CD8 + T lymphocytes was higher in PGs than in RCs (p = 0.129 and p = 0.541, respectively). Comparison of the number of CD57 + NK cells in atrophic and hyperplastic epithelium revealed a larger number of cells in the atrophic epithelium (p = 0.042). A larger number of CD57 + NK cells and CD8 + T lymphocytes were observed in grade III infiltrates compared to grade I/II (p = 0.145 and p = 0.725, respectively). CD8 + T lymphocytes were more prevalent than CD57 + NK cells in most cases when PGs and RCs were analyzed separately or in combination (p < 0.0001). CD57+ NK cells and CD8 + T lymphocytes play a key role in antiviral defense and the presence of these cells supports evidence suggesting the participation of these microorganisms in the pathogenesis of PGs and RCs. The response mediated by CD8 + T lymphocytes was more frequent, indicating greater participation of the adaptive immunity in these chronic lesions.

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“…15 In case of increased caspase-3 activity, B cell pr oliferation was decreased. It was demonstrated by the results of in vivo study on caspase-3-deficient mice that caspase-3 regulated homeostasis in B cell.…”

Section: 10mentioning

confidence: 99%

Caspase-3-dependent Cell Death in B lymphocyte Caused by Pseudomonas aeruginosa Pyocyanin

et al. 2015

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Pseudomonas aeruginosa is a Gram negative bacterium that can cause fatal infection in immunocompromised patient. This is an opportunist pathogen which is associated with some dental infections. Pseudomonas aeruginosa produces pyocyanin that functions as an important virulent factor in bacterial invasion. It can be identified in the lesion tissue and capable to induce cellular damage in endothelial cell, respiratory, neutrophil, and lymphocytes. B lymphocyte plays a significant role in the immune response of periapical infection; however, its cellular and molecular response to pyocyanin is unclear. Objective: To investigate cellular responses of B lymphocyte to the exposure of pyocyanin and the role of caspase-3 in its molecular mechanism. Methods: B lymphocytes (Raji cells) were cultured, and in five replications were exposed to various concentrations of pyocyanin for 24 h. MTT assay was performed to analyze the cytotoxicity effect of pyocyanin. Cell morphological analysis using phase contrast microscope were done in separate experiments. Immunocytochemical analysis was carried out for the identification of active caspase-3 protein expression, to study the mechanism involved in pyocyanin-induced cellular damage, Results: It showed that cell viability was decreased in pcyocyanin-treated groups. Pyocyanin induced cell death on B lymphocyte in a dose-dependent manner. Statistical analysis using ANOVA demonstrated significant difference between groups with p=0.000. Nuclear fragmentation was observed in pyocyanin-induced cell death; furthermore, caspase-3 was expressed clearly in cell cytoplasm after 24 h incubation. Conclusion: Pyocyanin is capable of inducing cell death on B lymphocyte. Caspase-3 may play an important role in the molecular mechanism of pyocyanin-induced cell death.

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Distribution of CD8 and CD20 lymphocytes in chronic periapical inflammatory lesions

Cited by 10 publications

References 16 publications

Immunohistochemical correlation between microvessel density and lymphoid infiltrate in radicular cysts

Immunohistochemical correlation between microvessel density and lymphoid infiltrate in radicular cysts

Analysis of CD57+ natural killer cells and CD8+ T lymphocytes in periapical granulomas and radicular cysts

Caspase-3-dependent Cell Death in B lymphocyte Caused by Pseudomonas aeruginosa Pyocyanin

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