Submucous implant of abrasive dental materials in rat tongues: clinical and histological evaluation

Pistóia, Alexandre Dorneles; Figueiredo, Maria Antonia Zancanaro de

doi:10.1590/s0103-64402002000200005

Cited by 3 publications

(5 citation statements)

References 6 publications

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“…The presence of fibrous tissue, lymphocytes and giant cells suggest that the immune system recognize the PLLA-g-HA and PLLA/HA scaffolds similarly, and provoke the encapsulation of the material to try to phagocytize and degrade the implant. Some authors suggest that the presence of fibrous tissue in implanted materials does not always imply an incompatibility with the host tissues [7,8]. In our study it was observed the presence of fibrous tissue in all samples at different times of material extraction.…”

Section: Resultssupporting

confidence: 61%

“…All experiments were approved by the Bioethics Committee of the Medicine and Psychology Faculty, Autonomous University of Baja California, Mexico, with registration number IORG # IORG007487, of United States Department of Health and Human Services (HHS). Also, the experiments were conducted in accordance with the Mexican Legislation Standard of NOM-063-SSA1-1993, Ley General de Salud [7].…”

Section: Methodsmentioning

confidence: 99%

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In Vivo Biocompatibility of Dental Scaffolds for Tissue Regeneration

Villarreal-Gómez¹,

Vera‐Graziano

Vega-Ríos³

et al. 2014

AMR

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It is important to develop scaffolds that resemble the extracellular matrix, thereby facilitating tissue regeneration. The objective of this work is to evaluate the biocompatibility of scaffolds of poly (L-lactide) with pure and grafted hydroxyapatite, at various concentrations of reinforcement, in Wistar Rat tissues, to evaluate the potential application on hard tissue regeneration. The biocompatibility tests were carried out in vivo in Wistar rats by implanting the material into the subcutaneous tissue and muscle from 1 to 14 weeks and evaluating the surrounding tissue stained with hematoxylin-eosin. All variants of scaffolds provoked an inflammatory response from mild to moderate, without showing necrosis. These results show that even if there is recognition of the implanted materials by the immune system, it does not provoke a violent response that damages the surrounding tissue, implying that the materials to be implanted for use in hard tissue can cause a mild reaction and tolerable long term effect that does not prevent their later use in hard tissue regeneration.

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Section: Resultssupporting

confidence: 61%

Section: Methodsmentioning

confidence: 99%

In Vivo Biocompatibility of Dental Scaffolds for Tissue Regeneration

Villarreal-Gómez¹,

Vera‐Graziano

Vega-Ríos³

et al. 2014

AMR

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show abstract

“…According to the previous publications there is strong evidence that PLLA/HA and PLLA/PLLA-g-HA scaffolds are biocompatible. Some authors suggest that the presence of granuloma in implanted materials does not always imply an incompatibility with the host tissues [7]. Such is the case of Branislav & Mirjana, who investigated the biocompatibility of different dental materials, intended for dental root canal obturation, by implantation in rat muscle tissue, through the analysis of different levels of inflammation of muscle tissue.…”

Section: Muscle Implantation Testmentioning

confidence: 99%

“…However, the cells do not possess the ability to grow in 3D orientations that define the anatomical shape of the tissue; instead, cells migrate to form random or two-dimensional (2D) layered fabrics. Despite this, the 3D structures are required, and this is accomplished by culturing the cells in three-dimensional porous structures known as "scaffolds", where the cells colonize and proliferate [7].…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility Evaluation of Electrospun Scaffolds of Poly (L-Lactide) with Pure and Grafted Hydroxyapatite

Villarreal-Gómez¹,

Vera‐Graziano²,

Vega-Ríos

et al. 2017

J. Mex. Chem. Soc.

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The objective of this work was to evaluate the biocompatibility of scaffolds of poly(L-lactide) with pure and grafted hydroxyapatite, at various concentrations of reinforcement. The biocompatibility tests were carried out in vivo in Wistar rats by implanting the material into the subcutaneous and muscle tissues from 1 to 14 weeks and evaluating the surrounding tissue stained with hematoxylin-eosin. For in vitro assays, MTT and neutral red assay were used to evaluate any cytotoxicity in Mioblast Muscle C2C12 Cells (ATCC® CRL-1772™) and Bovine Coronary Artery Endothelial Cells (BCAEC); Escherichia coli and Staphylococcus aureus were used to evaluate bacterial adhesion. All variants of scaffolds provoked a mild inflammatory response, without showing necrosis. No evidence of cytotoxicity was presented in cell viability tests and good bacterial cell adhesion was visualized for all of the materials studied.

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“…The pathogenesis of adverse reactions for these products is still unknown. When exogenous materials are injected into tissues, a granulomatous reaction with the presence of macrophages and the formation of new collagen surrounding the area can be observed. It is known that, during the initial period (4–6 months after injection), the PMMA spheres provoke a foreign body reaction and are later encapsulated by the collagen fibres of the tissue.…”

Section: Introductionmentioning

confidence: 99%