Antioxidant activity and phenolic composition of brazilian honeys and their extracts

Abstract

Foram avaliadas as atividades antioxidantes e o conteúdo de polifenóis de cinco méis silvestres e quatro de laranjeira, bem como de seus extratos fenólicos. A identificação e quantificação das substâncias fenólicas foram realizadas por HPLC-DAD (cromatografia líquida de alta eficiência com detecção por arranjo de diodos). A atividade sequestradora de radicais livres, determinada por 2,2-difenil-1-picrilhidrazil (DPPH •) e expressa como CE 50 , variou de 10,81 a 52,64 mg mL-1 para mel e de 6,17 a 52,87 mg mL-1 para extrato. A atividade antioxidante dos extratos foi também determinada pelos métodos com 2,2'-azinobis-(3-etil-benzotiazolino-6-ácido sulfônico) sal diamônio (ABTS) e poder antioxidante de redução do ferro (FRAP), e os resultados variaram de 46,53 a 383,49 mmol TE 100 g-1 e de 34,99 a 408,14 mol Fe(II) 100 g-1 , respectivamente. Também foram avaliados os teores de fenóis totais e flavonóides pelos métodos de Folin-Denis e complexação com cloreto de alumínio, respectivamente. A atividade antioxidante e o teor de fenóis totais mostraram-se altamente correlacionáveis. The antioxidant activities and total polyphenolic content of five multifloral and four orange blossom Brazilian honey samples and their phenolic extracts were investigated. Identification and quantification of phenolic compounds were carried out by HPLC-DAD (high-performance liquid chromatography with diode-array detection). The radical scavenging activities, determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH •) and expressed as EC 50 , ranged from 10.81 to 52.64 mg mL-1 for honey and 6.17 to 52.87 mg mL-1 for the extracts. The extract antioxidant activities were also determined by 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and ferric reducing antioxidant power (FRAP) assays, and the results varied from 46.53 to 383.49 mmol TE 100 g-1 and 34.99 to 408.14 mol Fe(II) 100 g-1 , respectively. It was also evaluated the total phenolic and flavonoid contents by the Folin-Denis and aluminum chloride methods, respectively. Antioxidant activities and total phenolic contents were found to be highly correlated.

Phenolic Composition and Antioxidant Properties of Brazilian Honeys

“…The levels of phenolic acids are higher than those of flavonoids in these samples. This trend was expected, and was previously observed by Sant'Anna et al 10 and Lianda et al 11 in Brazilian honeys. The results of TF contents obtained for honey extracts were lower than the values previously reported for other Brazilian honeys, 11 which ranged from 12.72 to 76.65 mg GAE 100 g -1 and 0.16 to 5.34 mg QE 100 g -1 .…”

“…This trend was expected, and was previously observed by Sant'Anna et al 10 and Lianda et al 11 in Brazilian honeys. The results of TF contents obtained for honey extracts were lower than the values previously reported for other Brazilian honeys, 11 which ranged from 12.72 to 76.65 mg GAE 100 g -1 and 0.16 to 5.34 mg QE 100 g -1 . The values of TP content obtained by Silva et al 12 for Brazilian melipona honey extracts were also higher than those found in this study.…”

Total phenols and antioxidant activities of natural honeys and propolis collected from different geographical regions of Ethiopia

“…One of the important functions of antioxidants in the living system is preventing the disturbance and functional loss of biological membranes and enzymes by scavenging the free radicals that otherwise induce oxidation of lipids, proteins, and DNA [17]. The antioxidant activity of phenolics is mainly due to their redox properties, which allows them to act as reducing agents [18,19]. To analyse the antioxidant activity of honey and propolis, the analytical methods commonly used refers to the sample's reducing capacity using the FolinCiocalteu or Folin-Denis and ferric reducing antioxidant power (FRAP) tests as well as antiradical activity with the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging assays [4].…”

Investigation of Antioxidant Capacity and Antimicrobial Potential of Pine Honey from Turkey Geography

“…Bu değerler, üretilen diğer monofloral ballar ile kıyaslandığında fenolik içeriği açısından kestane > çam > akasya > narenciye> şeklinde bir değerlendirme yapmak müm-kündür ( Tablo 3). 23,26,27,[37][38][39] Çalışma sonuçlarımız diğer ülkelerde çeşitli ballarda yapılan araştırma sonuçları ile karşılaştırıldığında, çam balına ait fenolik içeriğinin yüksek olduğu görülmektedir ( Tablo 3). 23,27,40 Çalışmada, flavonoid içeriği 1,24 ile 8,79 (QE/kg bal) arasında değişkenlik göstermektedir ve ortalama değeri 4,86±0,59 bulunmuştur.…”

“…Çalışma sonuçlarımız, diğer monofloral ballar ile kıyaslandığında flavonoid içeriğinin çam> kestane> akasya> ayçiçeği> narenciye şeklinde bir değere sahip olduğu görünmektedir. 37,38 Diğer ül-kelerde yapılan araştırma sonuçları ile karşılaştırıl-dığında ise türler arası ve bölgesel farklılığa bağlı değişkenlik göstermektedir (Tablo 3). 25,43 Çalışmada total antioksidan aktivitesinin hesaplanması, balın DPPH üzerinde serbest radikal süpü-Örnekler Total flavonoid (mgQE/kg) (min-max) Total fenolik mgGAE/kg) (min-max) Antioksidan, DPPH-sc 50 (mg/ml) (min-max) …”