Multivariate spectroscopic determination of the lamivudine-zidovudine association

Abstract

A determinação espectroscópica multivariada da associação lamivudina-zidovudina foi realizada por regressão de mínimos quadrados parciais. O modelo foi desenvolvido a partir de 20 misturas sintéticas utilizando-se dados espectrais centrados na média adquiridos entre 190 e 350 nm e 3 variáveis latentes. A validação externa foi realizada a partir de 6 misturas sintéticas, observando-se erros médios de previsão de aproximadamente 1%. Na análise de medicamentos foram obtidos bons resultados, com erros de previsão inferiores a 10%. A metodologia multivariada foi validada de acordo com os critérios do International Conference on Harmonization (ICH), demonstrando precisão, exatidão e robustez compatíveis com a legislação vigente.The multivariate spectroscopic determination of lamivudine-zidovudine associations was carried out by partial least square regression (PLS). This model was developed from 20 synthetic mixtures using mean-centered spectral data acquired from 190 to 350 nm and with 3 latent variables. External validation was performed with 6 synthetic mixtures providing prediction errors close to 1%. Moreover, the analysis of commercial drugs showed good results with prediction errors lower than 10%. The multivariate methodology was validated according to International Conference on Harmonization (ICH) criteria, demonstrating precision, accuracy and robustness within legal requirements.

Ultratrace Determination of the Antiretroviral Drug Lamivudine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry

“…1 Lamivudine; 2',3'-dideoxy-3'-thiacytidine, commonly called 3TC. Spectrophotometric (6)(7)(8)(9), chromatographic [10][11][12][13][14][15][16] and electrochemistry (17)(18) analytical methods have been developed for the determination of lamivudine. As a result of advancements in properties of the adsorptive stripping voltammetry, new methodologies have been developed for adenine, thymine, guanine, ATP, DNA and antiretroviral drugs determinations, employing alkaline solution with lower ionic strength as the supporting electrolyte (19)(20)(21)(22)(23)(24)(25).…”

Pharmaceutical Applications of Chemometric Techniques

“…Cordeiro et al conducted multivariate spectroscopic determination of lamivudine-zidovudine associations by partial least square regression (PLS). The multivariate methodology was validated according to the International Conference on Harmonization (ICH) criteria, demonstrating precision, accuracy, and robustness within legal requirements [73].…”

A process analytical technology approach for the production of fixed-dose combination tablets of zidovudine and lamivudine using near infrared spectroscopy and chemical images

“…In the scientific literature, some works can be found describing the use of HPLC and UVVis spectroscopy for the quantification of these drugs in pharmaceutical formulations [5][6][7][8][9][10][11][12]. These methods, however, are expensive, laborious, consume a considerable amount of reagent, and require many analytical manipulations.…”