Two novel eremophilane sesquiterpenes from an endophytic Xylariaceous fungus isolated from leaves of Cupressus lusitanica

Abstract

Dois novos sesquiterpenos eremofilanos, cupressolideo A e cupressolideo B, além de dois outros conhecidos, foram isolados a partir do extrato AcOEt do meio de cultura de uma espécie de Xylaria, isolada como fungo endofítico dos tecidos sadios das folhas de Cupressus lusitanica. Estudos espectroscópicos, usando EM e RMN, levaram às estruturas dos dois sesquiterpenos de esqueleto eremofilanos, novos na literatura.Two new eremophilane sesquiterpenes, cupressolide A and cupressolide B, along with two known sesquiterpenes, has been characterized from the EtOAc extract of a liquid medium where a Xylariaceous fungus, isolated as an endophytic fungus from health tissues of Cupressus lusitanica leaves, was cultivated. The structures of the isolated compounds were determined by analyses of their MS and NMR spectroscopic data.

Plant Bioactive Metabolites and Drugs Produced by Endophytic Fungi of Spermatophyta

“…francheti displayed a plant growth-promoting activity twice stronger than a wild-type of F. fujikuroi, due to the production of the physiologically [77] Mairetolide A Xylaria sp. C. lusitanica [78] Mairetolide F Xylaria sp. L. spinosa [76] Valencene M. albus C. zeylanicum [45] unidentified vine plant [79] Oryza granulata [62] Xylaria sp.…”

“…L. spinosa [76] Valencene M. albus C. zeylanicum [45] unidentified vine plant [79] Oryza granulata [62] Xylaria sp. C. lusitanica [78] Eudesmanes, eudesmenes (selinenes)…”

Endophytic fungi in economically important plants: ecological aspects, diversity and potential biotechnological applications

“…Endophytic fungi stand out among the microorganisms that have been prospected in search of natural products with biotechnological applications. The secondary metabolites produced by these fungi have been the focus of several studies and represent a promising source for the bioprospecting of novel molecules with potential applications in both the pharmaceutical industry as well as agricultural activities in the form of crop protection DAISY, 2003;AMARAL;RODRIGUES-FILHO, 2010;Kaushik, 2013;TALONTSI et al, 2013).…”

An HPLC Evaluation of Cytochalasin D Biosynthesis by Xylaria arbuscula Cultivated in Different Media

“…During studies of endophytic microorganisms associated with healthy leaf tissues of Cupressus lusitanica, some fungal species belonging to the Xylaria genus were isolated. This fungal genus produces a wide range of secondary metabolites, including cytochalasins [11,12]. One of these fungi, X. arbuscula (coded NICL5), has shown a remarkable ability to produce cytochalasins, mainly cytochalasin D ( Figure 1) [13].…”